ID K0RAM2_THAOC Unreviewed; 1459 AA.
AC K0RAM2;
DT 28-NOV-2012, integrated into UniProtKB/TrEMBL.
DT 28-NOV-2012, sequence version 1.
DT 24-JAN-2024, entry version 28.
DE RecName: Full=magnesium chelatase {ECO:0000256|ARBA:ARBA00012825};
DE EC=6.6.1.1 {ECO:0000256|ARBA:ARBA00012825};
GN ORFNames=THAOC_35247 {ECO:0000313|EMBL:EJK46106.1};
OS Thalassiosira oceanica (Marine diatom).
OC Eukaryota; Sar; Stramenopiles; Ochrophyta; Bacillariophyta;
OC Coscinodiscophyceae; Thalassiosirophycidae; Thalassiosirales;
OC Thalassiosiraceae; Thalassiosira.
OX NCBI_TaxID=159749 {ECO:0000313|EMBL:EJK46106.1, ECO:0000313|Proteomes:UP000266841};
RN [1] {ECO:0000313|EMBL:EJK46106.1, ECO:0000313|Proteomes:UP000266841}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCMP1005 {ECO:0000313|EMBL:EJK46106.1,
RC ECO:0000313|Proteomes:UP000266841};
RX PubMed=22835381; DOI=10.1186/gb-2012-13-7-r66;
RA Lommer M., Specht M., Roy A.S., Kraemer L., Andreson R., Gutowska M.A.,
RA Wolf J., Bergner S.V., Schilhabel M.B., Klostermeier U.C., Beiko R.G.,
RA Rosenstiel P., Hippler M., Laroche J.;
RT "Genome and low-iron response of an oceanic diatom adapted to chronic iron
RT limitation.";
RL Genome Biol. 13:R66-R66(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + Mg(2+) + protoporphyrin IX = ADP + 3 H(+) + Mg-
CC protoporphyrin IX + phosphate; Xref=Rhea:RHEA:13961,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:18420,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57306,
CC ChEBI:CHEBI:60492, ChEBI:CHEBI:456216; EC=6.6.1.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001499};
CC -!- PATHWAY: Porphyrin-containing compound metabolism.
CC {ECO:0000256|ARBA:ARBA00023444}.
CC -!- SIMILARITY: Belongs to the Mg-chelatase subunit H family.
CC {ECO:0000256|ARBA:ARBA00010851}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EJK46106.1}.
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DR EMBL; AGNL01047989; EJK46106.1; -; Genomic_DNA.
DR EnsemblProtists; EJK46106; EJK46106; THAOC_35247.
DR eggNOG; ENOG502RFFJ; Eukaryota.
DR OMA; GYWNAGG; -.
DR Proteomes; UP000266841; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016851; F:magnesium chelatase activity; IEA:UniProtKB-EC.
DR GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR CDD; cd10150; CobN_like; 1.
DR InterPro; IPR011771; BchH.
DR InterPro; IPR003672; CobN/Mg_chltase.
DR InterPro; IPR022571; Mg_chelatase_H_N.
DR NCBIfam; TIGR02025; BchH; 1.
DR PANTHER; PTHR44119; MAGNESIUM-CHELATASE SUBUNIT CHLH, CHLOROPLASTIC; 1.
DR PANTHER; PTHR44119:SF1; MAGNESIUM-CHELATASE SUBUNIT CHLH, CHLOROPLASTIC; 1.
DR Pfam; PF02514; CobN-Mg_chel; 1.
DR Pfam; PF11965; DUF3479; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Chlorophyll biosynthesis {ECO:0000256|ARBA:ARBA00023171};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Photosynthesis {ECO:0000256|ARBA:ARBA00022531};
KW Reference proteome {ECO:0000313|Proteomes:UP000266841};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..29
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 30..1459
FT /note="magnesium chelatase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003836173"
FT DOMAIN 106..271
FT /note="Magnesium chelatase subunit H N-terminal"
FT /evidence="ECO:0000259|Pfam:PF11965"
FT REGION 65..99
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1459 AA; 157260 MW; 245B8BA1668C1FAA CRC64;
MHSAIGTPPL AAALLLILQL LSGPSPLRAF APAGGVLLRT RPSIVRGRSP SRRGGVRVPA
RTVFEVERSP TREDEASSAP PPIAEGRGGA GDVDSDEGNT TRATNVVLLA GFESFNRELY
EQSGRLLPPS LGPVSVKVFA DSDIRGNASG EFATAVGEAD VFLASLVFDY DDVSAVTAML
DSAEDGRTQK TRLIFECATE LMEYNRVGSF SMASAGDGPS GPPPAVKAVL SKFSSGREED
KLAGYLELLK VGPELLKFVP GEKAGDLRTW MEAYRYWNQG GLSNVRSMLQ LLVSKMRKNA
AAAANAGDLT LPELVVTPAT GLLHPLLVDE SGSMAYASDP KSYMDWRLSS ACAKSAKERK
FPLAPDDAPR VALLLYRKHV ITGQRYIADL IRAMEAEGLM PVPVFINGVE AHAIVRDWLT
SDREVEKVNS GAATREPTYK PNEAAEVDAV VNTIGFPLVG GPAGSMEAGR NVDLSSKLLS
DMDVPYVVAS PLLLQSITTW KECGVLGLQS VVLYSLPELD GAVDTVVLGG LVGDKIALVP
ERVRKLAGRL NGWVGLRRTP VKDRRISVVL YGFPPNVGAV GTAALLDVPS SLDNLLKRLD
KEGYNVGKYA SDPNSSGESL VAALSVLCEN AVIAGGFERM QSAVEVRMER ARGGDPTVAE
TLARPGGGLG GAVVKGANVE FDDLENMLGK YMARKVRRAW PESERGPGVN AKNEMVVSGL
QLGNVFVTVQ PLLGVEGDPM RLLFERDLTP HPQYCAAYKY MQSGEDEGGA GSQAVVHLGM
HGTVEWLPGQ PLGNDRQSWS DELLGSLPNV YVYAANNPSE SILAKRRGYG TLVSYNVPPY
GRAGLYLELA SLKDLVNEFR SDSAESRADL RESILAQCER TGLTSDISPP CDDADEESFD
DYITSLSNYL VELEERLFSS GLHTLGDAPT DLELASYLNA YFAGRLNDAE VEDVVRQSRV
KRERTDENAF NVLAFLAWLA DLAGGGARED AVGDVSDEKA SLMQEGLEVA DLLKKNAEEL
NSVMTALDGG YVKPAPGGDL LRDGTSVLPT GRNIHALDPY RMPSAGASAR GTRAADEIIR
QHLEANGGVY PETVAVTLWG LDTIKTRGEA IAIVLALVGA RAVREGTGRT VCFELIPLEE
LGRPRIDILA SLSGIFRDSF ANVVDLLDDM FERAATADES PNMNFIRKHA AELEAAGTER
PAARLFSNPP GDYGSMVNEV VGTGDWEDEA SLGEVWKSRN AYSYGRSEGG GGTKSGTSRP
EVLNKLLATT ERIVQEIDSV EYGLTDIQEY YSNTGALKKA AENRKPIDKS TGKAQKVAVS
VIEAFGGPRT DDDCVPVRDV EEVLRIEYRS KLLNPKWRDA MLEQGSGGAY EVSQRMTAMI
GWAATAEVDN FVFDQAAERY ALDEEVAAKL QKNNPEAFKN VVRRLLEASG RGMWETDDET
IEKLKSLYAD ADDLVEQVR
//
