ID   K1XDU6_9BACT            Unreviewed;       479 AA.
AC   K1XDU6;
DT   28-NOV-2012, integrated into UniProtKB/TrEMBL.
DT   28-NOV-2012, sequence version 1.
DT   27-MAR-2024, entry version 27.
DE   SubName: Full=Radical SAM protein {ECO:0000313|EMBL:EKD28400.1};
GN   ORFNames=ACD_79C00311G0006 {ECO:0000313|EMBL:EKD28400.1};
OS   uncultured bacterium.
OC   Bacteria; environmental samples.
OX   NCBI_TaxID=77133 {ECO:0000313|EMBL:EKD28400.1};
RN   [1] {ECO:0000313|EMBL:EKD28400.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=23019650; DOI=10.1126/science.1224041;
RA   Wrighton K.C., Thomas B.C., Sharon I., Miller C.S., Castelle C.J.,
RA   VerBerkmoes N.C., Wilkins M.J., Hettich R.L., Lipton M.S., Williams K.H.,
RA   Long P.E., Banfield J.F.;
RT   "Fermentation, hydrogen, and sulfur metabolism in multiple uncultivated
RT   bacterial phyla.";
RL   Science 337:1661-1665(2012).
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|ARBA:ARBA00001966};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:EKD28400.1}.
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DR   EMBL; AMFJ01034780; EKD28400.1; -; Genomic_DNA.
DR   AlphaFoldDB; K1XDU6; -.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   CDD; cd01335; Radical_SAM; 1.
DR   CDD; cd02068; radical_SAM_B12_BD; 1.
DR   Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR   Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR   InterPro; IPR006158; Cobalamin-bd.
DR   InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR   InterPro; IPR034466; Methyltransferase_Class_B.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR023404; rSAM_horseshoe.
DR   PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR   PANTHER; PTHR43409:SF12; BLL1977 PROTEIN; 1.
DR   Pfam; PF02310; B12-binding; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SMART; SM00729; Elp3; 1.
DR   SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR   PROSITE; PS51332; B12_BINDING; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   4: Predicted;
KW   4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW   Iron {ECO:0000256|ARBA:ARBA00022485};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022485}.
FT   DOMAIN          19..151
FT                   /note="B12-binding"
FT                   /evidence="ECO:0000259|PROSITE:PS51332"
FT   DOMAIN          197..421
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000259|PROSITE:PS51918"
SQ   SEQUENCE   479 AA;  55097 MW;  6A320702479E044F CRC64;
     MKIPIEVKKI ALIYSPIAEG LALLSTVQKV QRRPGLGLRY IKSILLEQNI DVDLYDNLYD
     VNAADVLFNI LNQKKYDLIG FHTTSASIPS VLKTISKLNS EWYSGRIIAG GPGTLHFKKL
     LESGIDVIVF GEGEETIKHV INGLKGNCSL NTISGIAFYE NNNLIITNKS DIVDLNRIPY
     PYWEKNNNKI YGDLFNITIK KPYYVVMASR GCPFNCSFCS THLYWRKKYR LREVNNVIDE
     IKFLITKRNA KYIHFLDDIF GLNPKWIEEF IIEVKKTKLK FNFSIVLHPM SYRNNRYSIF
     SGLKDIGCSL ISFGAQSANK DILKNINRLE CEPQELEDAI SKTNNLNIVS VLTYILGLPG
     ETRETIKETL QFVNKVKPTI VDFHPLLYLP GSEIVESFSK DQYCKISDSE LSRLCLYSSF
     NYYILHGGLV RVLMYIVKNN LSWFLNLFDI ISYIFQFMKQ INSKSLTTKY FDKLNSKIN
//