ID K2GFH2_9BACT Unreviewed; 378 AA.
AC K2GFH2;
DT 28-NOV-2012, integrated into UniProtKB/TrEMBL.
DT 28-NOV-2012, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE SubName: Full=NADP-dependent malic enzyme {ECO:0000313|EMBL:EKE29059.1};
GN ORFNames=ACD_2C00239G0001 {ECO:0000313|EMBL:EKE29059.1};
OS uncultured bacterium (gcode 4).
OC Bacteria; environmental samples.
OX NCBI_TaxID=1234023 {ECO:0000313|EMBL:EKE29059.1};
RN [1] {ECO:0000313|EMBL:EKE29059.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=23019650; DOI=10.1126/science.1224041;
RA Wrighton K.C., Thomas B.C., Sharon I., Miller C.S., Castelle C.J.,
RA VerBerkmoes N.C., Wilkins M.J., Hettich R.L., Lipton M.S., Williams K.H.,
RA Long P.E., Banfield J.F.;
RT "Fermentation, hydrogen, and sulfur metabolism in multiple uncultivated
RT bacterial phyla.";
RL Science 337:1661-1665(2012).
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EKE29059.1}.
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DR EMBL; AMFJ01000239; EKE29059.1; -; Genomic_DNA.
DR AlphaFoldDB; K2GFH2; -.
DR GO; GO:0004470; F:malic enzyme activity; IEA:InterPro.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR CDD; cd05311; NAD_bind_2_malic_enz; 1.
DR Gene3D; 3.40.50.10380; Malic enzyme, N-terminal domain; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR046346; Aminoacid_DH-like_N_sf.
DR InterPro; IPR012301; Malic_N_dom.
DR InterPro; IPR037062; Malic_N_dom_sf.
DR InterPro; IPR012302; Malic_NAD-bd.
DR InterPro; IPR045213; Malic_NAD-bd_bact_type.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR43237; NADP-DEPENDENT MALIC ENZYME; 1.
DR PANTHER; PTHR43237:SF4; NADP-DEPENDENT MALIC ENZYME; 1.
DR Pfam; PF00390; malic; 1.
DR Pfam; PF03949; Malic_M; 1.
DR SMART; SM01274; malic; 1.
DR SMART; SM00919; Malic_M; 1.
DR SUPFAM; SSF53223; Aminoacid dehydrogenase-like, N-terminal domain; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 4: Predicted;
FT DOMAIN 15..148
FT /note="Malic enzyme N-terminal"
FT /evidence="ECO:0000259|SMART:SM01274"
FT DOMAIN 160..377
FT /note="Malic enzyme NAD-binding"
FT /evidence="ECO:0000259|SMART:SM00919"
SQ SEQUENCE 378 AA; 43415 MW; 1079B1FF481BA578 CRC64;
MNYFEESLEL HEKLHWKLEV ISKMPILTRL DLSLAYTPGV AAPCLKIAEN KKDAYKYTIK
SNTIAVISDW SAVLWLGNIG PEAGLPVMEG KCILLKEFAW VNAFPIMLDT QDTEEIIKTI
KNIAPTFWGI NLEDISAPRC FEIEERLKSE LDIPVFHDDQ HWTAIVCLAW IINWLKITGK
KKENLKVVIN WLWAAWTAIL KLLKLYWVWN VIACDSRWIV SRNREDLNAE KIKILDIVNN
DNEDWTLSDA IVRADIFIWV SVAWALSKED VMKMNIDSMI FAMANPAPEI LPDLAKEWGA
RIVATGRSDF PNQLNNVLVF PWIFKWALMY GIPQITDDMK LNAAQALADY VKNPTEDMII
PDPLDKRVVD VIADSLKY
//
