ID K2KKE4_9GAMM Unreviewed; 472 AA.
AC K2KKE4;
DT 28-NOV-2012, integrated into UniProtKB/TrEMBL.
DT 28-NOV-2012, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE SubName: Full=Arabinan endo-1,5-alpha-L-arabinosidase {ECO:0000313|EMBL:EKE77865.1};
GN ORFNames=B3C1_00355 {ECO:0000313|EMBL:EKE77865.1};
OS Gallaecimonas xiamenensis 3-C-1.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Gallaecimonas.
OX NCBI_TaxID=745411 {ECO:0000313|EMBL:EKE77865.1, ECO:0000313|Proteomes:UP000006755};
RN [1] {ECO:0000313|EMBL:EKE77865.1, ECO:0000313|Proteomes:UP000006755}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3-C-1 {ECO:0000313|EMBL:EKE77865.1,
RC ECO:0000313|Proteomes:UP000006755};
RX PubMed=23209203; DOI=10.1128/JB.01854-12;
RA Lai Q., Wang L., Wang W., Shao Z.;
RT "Genome Sequence of Gallaecimonas xiamenensis Type Strain 3-C-1.";
RL J. Bacteriol. 194:6937-6937(2012).
CC -!- PATHWAY: Glycan metabolism; L-arabinan degradation.
CC {ECO:0000256|ARBA:ARBA00004834}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EKE77865.1}.
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DR EMBL; AMRI01000001; EKE77865.1; -; Genomic_DNA.
DR AlphaFoldDB; K2KKE4; -.
DR STRING; 745411.B3C1_00355; -.
DR PATRIC; fig|745411.4.peg.60; -.
DR eggNOG; COG3507; Bacteria.
DR Proteomes; UP000006755; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd18829; GH43_BsArb43A-like; 1.
DR CDD; cd00161; RICIN; 1.
DR Gene3D; 2.80.10.50; -; 3.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR InterPro; IPR035992; Ricin_B-like_lectins.
DR InterPro; IPR000772; Ricin_B_lectin.
DR PANTHER; PTHR43301; ARABINAN ENDO-1,5-ALPHA-L-ARABINOSIDASE; 1.
DR PANTHER; PTHR43301:SF3; ARABINOSIDASE-RELATED; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR Pfam; PF14200; RicinB_lectin_2; 2.
DR SMART; SM00458; RICIN; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF50370; Ricin B-like lectins; 1.
DR PROSITE; PS50231; RICIN_B_LECTIN; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|RuleBase:RU361187};
KW Hydrolase {ECO:0000256|RuleBase:RU361187};
KW Reference proteome {ECO:0000313|Proteomes:UP000006755};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..34
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 35..472
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003859999"
FT DOMAIN 40..177
FT /note="Ricin B lectin"
FT /evidence="ECO:0000259|SMART:SM00458"
FT ACT_SITE 197
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 363
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 314
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 472 AA; 51167 MW; 46D555E5F0CA785A CRC64;
MTAQGRQYKH NKDNLMFSRK LALGLLCLPL AASAIQPIED GTYVIRNKAT GKVLDLVNGN
AADGTNIIQY SQTGASNQHW QVNYLGDGRY RLVNQAGGGA AEVYGWDNSD GANISQWASN
GLAIQQWLLT DQGDGTFKVV NVHTGKAMEV YNWDSSDGAN VSQWTDNGLD IQRWSFESLD
TGNAYHWPLS GNLGTHDPTA MAENGTWYEF QTGPGIYRKV SFDGGLNWEP LPSVLPDGLG
WWKNYVPDQE GIDVWAPDVK AYNGKVWLYY SISTFGSRVS AIGLLSAPSL ASDSWTDQGL
VLNTTASSSY NAIDPDLVID KDNKPWLVLG SWSSGIKLTR LNASTMKPSG SLYSLASRTG
GIEAPSITYR SGYYYLFVST GVCCAGTDST YQIRYGRSTS ITGPYLDKNG VDMMNGGGSL
LDGGNSQWVG PGGQDLVGNG VIVRHAYDAS DNGNAKLLIS KLNWDSAGWP RY
//
