ID K2LAG4_HELPX Unreviewed; 681 AA.
AC K2LAG4;
DT 28-NOV-2012, integrated into UniProtKB/TrEMBL.
DT 28-NOV-2012, sequence version 1.
DT 27-MAR-2024, entry version 43.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=OUK_0117 {ECO:0000313|EMBL:EKE91649.1};
OS Helicobacter pylori R037c.
OC Bacteria; Campylobacterota; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=1145114 {ECO:0000313|EMBL:EKE91649.1, ECO:0000313|Proteomes:UP000006760};
RN [1] {ECO:0000313|EMBL:EKE91649.1, ECO:0000313|Proteomes:UP000006760}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=R037c {ECO:0000313|EMBL:EKE91649.1,
RC ECO:0000313|Proteomes:UP000006760};
RX PubMed=23661595; DOI=10.1111/2049-632X.12045;
RA Blanchard T.G., Czinn S.J., Correa P., Nakazawa T., Keelan M.,
RA Morningstar L., Santana-Cruz I., Maroo A., McCracken C., Shefchek K.,
RA Daugherty S., Song Y., Fraser C.M., Fricke W.F.;
RT "Genome sequences of 65 Helicobacter pylori strains isolated from
RT asymptomatic individuals and patients with gastric cancer, peptic ulcer
RT disease, or gastritis.";
RL Pathog. Dis. 68:39-43(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EKE91649.1}.
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DR EMBL; AMOU01000001; EKE91649.1; -; Genomic_DNA.
DR RefSeq; WP_001913418.1; NZ_AMOU01000001.1.
DR AlphaFoldDB; K2LAG4; -.
DR PATRIC; fig|1145114.3.peg.114; -.
DR Proteomes; UP000006760; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 9..270
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 271..539
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 30..37
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 681 AA; 77457 MW; 5629AC48E62E11C3 CRC64;
MDFEKSILGN LNEAQKIAAS HIQGPLLILA GAGSGKTKTL TSRLAYLIGA CGVPSENTLT
LTFTNKASKE MQERALKLLN NQAFIPPLLC TFHRFGLLFL RQHMNLLKRA CDFSVLDSDE
VKTLCKQLKI SNFRASISQI KNGMMDLSMQ DSECYKAYEL YQNALKKDNL VDFDDLLCLS
LKILQDNEKL AKEISERYHY IMVDEYQDTN ALQLEFLKQL SCAHHNLCVV GDDDQSIYGF
RGADISNILN FSKHFKGAKV VKLETNYRSS AEILACANSL ISHNQHRHTK TLQSFKGSHK
SVVCKEYLTQ KEESLDVAYQ IKALLKKGEN LENIAILYRL NGLSRSIEES LNALNIPYRL
IGAVSFYERA EVKDALAFMH LVAKKDDRFF IKRVLNKPPR GLGKITQEWI FSLLDGEDLN
LEEALKLGAF KDKLNPKNEH ALNKFIAMIG RLREAFEISV EEFCARFLEE TNLLKSYEKE
DNYEEREGFV KELLSLVKEH FKTNPTHSLL DFLNESVLDV HNTENTQKVS CMSVHMSKGL
EFKHVFVIGL EEGFFPHRGF NQESDLEEER RLAYVAITRA KEGLQLSYVQ ERSYFGRKIS
CSPSVFLEEA KLLNQDKPPK QDHQKDAPIK VGDLIRHKIF GTGRVLGVEK GLSGLCLKIN
CGGNVYDKIS EKFVEKVDNG F
//
