ID K2RLX8_MACPH Unreviewed; 620 AA.
AC K2RLX8;
DT 28-NOV-2012, integrated into UniProtKB/TrEMBL.
DT 28-NOV-2012, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=L-ornithine N(5)-monooxygenase {ECO:0000256|ARBA:ARBA00018612};
DE EC=1.14.13.196 {ECO:0000256|ARBA:ARBA00012881};
DE AltName: Full=L-ornithine N(5)-oxygenase {ECO:0000256|ARBA:ARBA00030351};
GN ORFNames=MPH_11787 {ECO:0000313|EMBL:EKG11169.1};
OS Macrophomina phaseolina (strain MS6) (Charcoal rot fungus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Dothideomycetes incertae sedis; Botryosphaeriales; Botryosphaeriaceae;
OC Macrophomina.
OX NCBI_TaxID=1126212 {ECO:0000313|EMBL:EKG11169.1, ECO:0000313|Proteomes:UP000007129};
RN [1] {ECO:0000313|EMBL:EKG11169.1, ECO:0000313|Proteomes:UP000007129}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MS6 {ECO:0000313|EMBL:EKG11169.1,
RC ECO:0000313|Proteomes:UP000007129};
RX PubMed=22992219; DOI=10.1186/1471-2164-13-493;
RA Islam M.S., Haque M.S., Islam M.M., Emdad E.M., Halim A., Hossen Q.M.M.,
RA Hossain M.Z., Ahmed B., Rahim S., Rahman M.S., Alam M.M., Hou S., Wan X.,
RA Saito J.A., Alam M.;
RT "Tools to kill: Genome of one of the most destructive plant pathogenic
RT fungi Macrophomina phaseolina.";
RL BMC Genomics 13:493-493(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-ornithine + NADH + O2 = H2O + N(5)-hydroxy-L-ornithine +
CC NAD(+); Xref=Rhea:RHEA:41512, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:46911, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC ChEBI:CHEBI:78275; EC=1.14.13.196;
CC Evidence={ECO:0000256|ARBA:ARBA00001398};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-ornithine + NADPH + O2 = H2O + N(5)-hydroxy-L-ornithine +
CC NADP(+); Xref=Rhea:RHEA:41508, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:46911, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC ChEBI:CHEBI:78275; EC=1.14.13.196;
CC Evidence={ECO:0000256|ARBA:ARBA00001847};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- PATHWAY: Siderophore biosynthesis. {ECO:0000256|ARBA:ARBA00004924}.
CC -!- SIMILARITY: Belongs to the lysine N(6)-hydroxylase/L-ornithine N(5)-
CC oxygenase family. {ECO:0000256|ARBA:ARBA00007588}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EKG11169.1}.
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DR EMBL; AHHD01000498; EKG11169.1; -; Genomic_DNA.
DR AlphaFoldDB; K2RLX8; -.
DR VEuPathDB; FungiDB:MPH_11787; -.
DR eggNOG; KOG1399; Eukaryota.
DR HOGENOM; CLU_006937_6_1_1; -.
DR InParanoid; K2RLX8; -.
DR OrthoDB; 49786at2759; -.
DR Proteomes; UP000007129; Unassembled WGS sequence.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0044249; P:cellular biosynthetic process; IEA:UniProt.
DR GO; GO:1901566; P:organonitrogen compound biosynthetic process; IEA:UniProt.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 2.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR025700; Lys/Orn_oxygenase.
DR PANTHER; PTHR42877; -; 1.
DR PANTHER; PTHR42877:SF7; FLAVIN-BINDING MONOOXYGENASE-RELATED; 1.
DR Pfam; PF13434; Lys_Orn_oxgnase; 1.
DR Pfam; PF13450; NAD_binding_8; 1.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
PE 3: Inferred from homology;
KW Monooxygenase {ECO:0000313|EMBL:EKG11169.1};
KW Oxidoreductase {ECO:0000313|EMBL:EKG11169.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000007129}.
FT REGION 1..52
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 6..52
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 620 AA; 69080 MW; 4A29AA8C6896B992 CRC64;
MSAVPSLPES LATPSTNGLP ASSSSSSPPT TNGINGHTTS SKPTNPTTFQ LHNAPVENLR
PLRVIVIGAG YSGIYCGIRI PERLRNVSLT IYEKNAGVGG AWWENRYPGC ACDIPSHSYQ
YSFAPNVEWS SLYAPSREIQ AYLERTAREY GADRFLRLKH RVKGCRWDGG EGVWRVEVED
LDAGRTFVDT AEVVVSARGN LNEPAWPEIE GLKGFEGEVM HSATWKEEYD FSNKRIGVIG
SGSSAIQIIP RLQKVPGTQL SCFIRSKTWI SPPFGQAMWD KLGLTSFIIP DDLKAKFKSD
PAHYHKFRIA IEEDSNSIHA VTIRGTEMAV KAKKTFEENM RARLASKPDI FAALLPSFSP
GCRRLTPGPG FLEALTEPNV AFVTDPITRI EPRGVRTNDG KLHEIDALVC ATGFHVSAPP
PFPVVGAGGR TLQDHWTRRA TSYLGLATDG FPNHFMMLGP NSAIGSGSLT MMIESTGDYV
IKCIRKIQKD NIRSMAVQPR RVRDFIEYAD TYFEGTVFKD ECKSWYRNAE NQITGLWPGS
TLHCIEALRS PRWEDWEYEY VGEEGNGEEG NRMAWLGNGW STKEKEGKDL AFYLEPEFLD
KPLAGKPEDK EVYKVRPFSY
//
