ID K5V3U0_PHACS Unreviewed; 488 AA.
AC K5V3U0;
DT 09-JAN-2013, integrated into UniProtKB/TrEMBL.
DT 09-JAN-2013, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=(4-O-methyl)-D-glucuronate--lignin esterase {ECO:0000256|ARBA:ARBA00026105};
DE EC=3.1.1.117 {ECO:0000256|ARBA:ARBA00026105};
GN ORFNames=PHACADRAFT_160751 {ECO:0000313|EMBL:EKM57251.1};
OS Phanerochaete carnosa (strain HHB-10118-sp) (White-rot fungus) (Peniophora
OS carnosa).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Polyporales; Phanerochaetaceae; Phanerochaete.
OX NCBI_TaxID=650164 {ECO:0000313|EMBL:EKM57251.1, ECO:0000313|Proteomes:UP000008370};
RN [1] {ECO:0000313|EMBL:EKM57251.1, ECO:0000313|Proteomes:UP000008370}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HHB-10118-sp {ECO:0000313|EMBL:EKM57251.1,
RC ECO:0000313|Proteomes:UP000008370};
RX PubMed=22937793; DOI=10.1186/1471-2164-13-444;
RA Suzuki H., MacDonald J., Syed K., Salamov A., Hori C., Aerts A.,
RA Henrissat B., Wiebenga A., vanKuyk P.A., Barry K., Lindquist E.,
RA LaButti K., Lapidus A., Lucas S., Coutinho P., Gong Y., Samejima M.,
RA Mahadevan R., Abou-Zaid M., de Vries R.P., Igarashi K., Yadav J.S.,
RA Grigoriev I.V., Master E.R.;
RT "Comparative genomics of the white-rot fungi, Phanerochaete carnosa and P.
RT chrysosporium, to elucidate the genetic basis of the distinct wood types
RT they colonize.";
RL BMC Genomics 13:444-444(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 4-O-methyl-alpha-D-glucuronosyl ester derivative + H2O = 4-
CC O-methyl-alpha-D-glucuronate derivative + an alcohol + H(+);
CC Xref=Rhea:RHEA:67452, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30879, ChEBI:CHEBI:171667, ChEBI:CHEBI:171668;
CC EC=3.1.1.117; Evidence={ECO:0000256|ARBA:ARBA00024511};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:67453;
CC Evidence={ECO:0000256|ARBA:ARBA00024511};
CC -!- SIMILARITY: Belongs to the carbohydrate esterase 15 (CE15) family.
CC {ECO:0000256|ARBA:ARBA00010092}.
CC -!- SIMILARITY: Belongs to the peptidase S10 family.
CC {ECO:0000256|ARBA:ARBA00009431}.
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DR EMBL; JH930471; EKM57251.1; -; Genomic_DNA.
DR RefSeq; XP_007395069.1; XM_007395007.1.
DR AlphaFoldDB; K5V3U0; -.
DR GeneID; 18909172; -.
DR KEGG; pco:PHACADRAFT_160751; -.
DR HOGENOM; CLU_008523_10_3_1; -.
DR InParanoid; K5V3U0; -.
DR OrthoDB; 1647009at2759; -.
DR Proteomes; UP000008370; Unassembled WGS sequence.
DR GO; GO:0004185; F:serine-type carboxypeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 1.10.287.410; -; 1.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR001563; Peptidase_S10.
DR InterPro; IPR033124; Ser_caboxypep_his_AS.
DR PANTHER; PTHR11802:SF64; CARBOXYPEPTIDASE 2-RELATED; 1.
DR PANTHER; PTHR11802; SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE; 1.
DR Pfam; PF00450; Peptidase_S10; 1.
DR PRINTS; PR00724; CRBOXYPTASEC.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
DR PROSITE; PS00560; CARBOXYPEPT_SER_HIS; 1.
PE 3: Inferred from homology;
KW Carboxypeptidase {ECO:0000256|ARBA:ARBA00022645};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000008370};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..24
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 25..488
FT /note="(4-O-methyl)-D-glucuronate--lignin esterase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003884448"
SQ SEQUENCE 488 AA; 53461 MW; 32C99EA8BF9D543A CRC64;
MARFSRSLKL ALLGLAFVVV GTVGVVVDAV NHVRSTSTPS LNFETFTSEA QPDVGLRFIR
NSGVCETTPG VDQLSGYIDF GANMSMWFWF FESRHEPETA PFTLWLNGGP GCSSMIGLFQ
EHGPCHVLED GETTVLNPFS WNNITNIIYV DQPIGTGFSH GTIDVNSTFA SAPAFWSAFQ
VLLESQEFAK YESREFIFAT ESYGGHYGPE FVTFFDEQNT KIDQGSVNGE KINVSRLMIN
NGWYDPLLQN EAYVTFATNA PGYGQLQNDS VIAALNQAFF GSGGCQEQEL ACYAAGNGTN
SNEVCMQADN FCIDNVFVPA VGDRDSDDLR QNSSALFPSE SYVTFLRNAT IMELIGAETR
YTECSNPVDI QFSKTGDDAR TLLPQLGALV SSGLKTLIWA GDADINCNWL GGHASVLAMD
WFGNETLHRT PFTNITIRGQ PVAAVQNVDS FSFARVYQAG HEIPAFQPEA AFEIFSQVVR
GEQLHSVS
//
