ID K7LF42_SOYBN Unreviewed; 332 AA.
AC K7LF42;
DT 09-JAN-2013, integrated into UniProtKB/TrEMBL.
DT 09-JAN-2013, sequence version 1.
DT 27-MAR-2024, entry version 49.
DE RecName: Full=Glycerol-3-phosphate acyltransferase, chloroplastic {ECO:0000256|PIRNR:PIRNR000431};
DE Short=GPAT {ECO:0000256|PIRNR:PIRNR000431};
DE EC=2.3.1.15 {ECO:0000256|PIRNR:PIRNR000431};
GN Name=100800190 {ECO:0000313|EnsemblPlants:KRH39589};
GN ORFNames=GLYMA_09G207900 {ECO:0000313|EMBL:KRH39589.1};
OS Glycine max (Soybean) (Glycine hispida).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3847 {ECO:0000313|EnsemblPlants:KRH39589};
RN [1] {ECO:0000313|EMBL:KRH39589.1, ECO:0000313|EnsemblPlants:KRH39589}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Williams 82 {ECO:0000313|EnsemblPlants:KRH39589};
RC TISSUE=Callus {ECO:0000313|EMBL:KRH39589.1};
RX PubMed=20075913; DOI=10.1038/nature08670;
RA Schmutz J., Cannon S.B., Schlueter J., Ma J., Mitros T., Nelson W.,
RA Hyten D.L., Song Q., Thelen J.J., Cheng J., Xu D., Hellsten U., May G.D.,
RA Yu Y., Sakurai T., Umezawa T., Bhattacharyya M.K., Sandhu D.,
RA Valliyodan B., Lindquist E., Peto M., Grant D., Shu S., Goodstein D.,
RA Barry K., Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N.,
RA Joshi T., Libault M., Sethuraman A., Zhang X.-C., Shinozaki K.,
RA Nguyen H.T., Wing R.A., Cregan P., Specht J., Grimwood J., Rokhsar D.,
RA Stacey G., Shoemaker R.C., Jackson S.A.;
RT "Genome sequence of the palaeopolyploid soybean.";
RL Nature 463:178-183(2010).
RN [2] {ECO:0000313|EnsemblPlants:KRH39589}
RP IDENTIFICATION.
RC STRAIN=Williams 82 {ECO:0000313|EnsemblPlants:KRH39589};
RG EnsemblPlants;
RL Submitted (FEB-2018) to UniProtKB.
RN [3] {ECO:0000313|EMBL:KRH39589.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Callus {ECO:0000313|EMBL:KRH39589.1};
RA Schmutz J., Cannon S., Schlueter J., Ma J., Mitros T., Nelson W., Hyten D.,
RA Song Q., Thelen J., Cheng J., Xu D., Hellsten U., May G., Yu Y.,
RA Sakurai T., Umezawa T., Bhattacharyya M., Sandhu D., Valliyodan B.,
RA Lindquist E., Peto M., Grant D., Shu S., Goodstein D., Barry K.,
RA Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N., Joshi T.,
RA Libault M., Sethuraman A., Zhang X., Shinozaki K., Nguyen H., Wing R.,
RA Cregan P., Specht J., Grimwood J., Rokhsar D., Stacey G., Shoemaker R.,
RA Jackson S.;
RT "WGS assembly of Glycine max.";
RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Esterifies acyl-group from acyl-ACP to the sn-1 position of
CC glycerol-3-phosphate. The enzyme from chilling-resistant plants
CC discriminates against non-fluid palmitic acid and selects oleic acid
CC whereas the enzyme from sensitive plants accepts both fatty acids.
CC {ECO:0000256|PIRNR:PIRNR000431}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an acyl-CoA + sn-glycerol 3-phosphate = a 1-acyl-sn-glycero-3-
CC phosphate + CoA; Xref=Rhea:RHEA:15325, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57597, ChEBI:CHEBI:57970, ChEBI:CHEBI:58342; EC=2.3.1.15;
CC Evidence={ECO:0000256|PIRNR:PIRNR000431};
CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}.
CC -!- PATHWAY: Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-
CC diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
CC {ECO:0000256|ARBA:ARBA00004765, ECO:0000256|PIRNR:PIRNR000431}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma
CC {ECO:0000256|ARBA:ARBA00004470, ECO:0000256|PIRNR:PIRNR000431}.
CC -!- SIMILARITY: Belongs to the GPAT/DAPAT family.
CC {ECO:0000256|ARBA:ARBA00007937, ECO:0000256|PIRNR:PIRNR000431}.
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DR EMBL; CM000842; KRH39589.1; -; Genomic_DNA.
DR EMBL; CM000842; KRH39590.1; -; Genomic_DNA.
DR AlphaFoldDB; K7LF42; -.
DR EnsemblPlants; KRH39589; KRH39589; GLYMA_09G207900.
DR EnsemblPlants; KRH39590; KRH39590; GLYMA_09G207900.
DR Gramene; KRH39589; KRH39589; GLYMA_09G207900.
DR Gramene; KRH39590; KRH39590; GLYMA_09G207900.
DR HOGENOM; CLU_043091_1_0_1; -.
DR UniPathway; UPA00557; UER00612.
DR Proteomes; UP000008827; Chromosome 9.
DR ExpressionAtlas; K7LF42; baseline.
DR GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR GO; GO:0004366; F:glycerol-3-phosphate O-acyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0102420; F:sn-1-glycerol-3-phosphate C16:0-DCA-CoA acyl transferase activity; IEA:UniProtKB-EC.
DR GO; GO:0016024; P:CDP-diacylglycerol biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.1130.10; Glycerol-3-phosphate (1)-acyltransferase; 1.
DR Gene3D; 1.10.1200.50; Glycerol-3-phosphate acyltransferase, alpha helical bundle, N-terminal; 1.
DR InterPro; IPR016222; G3P_O-acylTrfase_chlp.
DR InterPro; IPR023083; G3P_O-acylTrfase_N.
DR InterPro; IPR038114; GPAT_N_sf.
DR InterPro; IPR002123; Plipid/glycerol_acylTrfase.
DR PANTHER; PTHR35695; GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE, CHLOROPLASTIC; 1.
DR PANTHER; PTHR35695:SF1; GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE, CHLOROPLASTIC; 1.
DR Pfam; PF01553; Acyltransferase; 1.
DR Pfam; PF14829; GPAT_N; 1.
DR PIRSF; PIRSF000431; Glycerol-3-P_O-acyltransfrase; 1.
DR SMART; SM00563; PlsC; 1.
DR SUPFAM; SSF69593; Glycerol-3-phosphate (1)-acyltransferase; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315,
KW ECO:0000256|PIRNR:PIRNR000431};
KW Chloroplast {ECO:0000256|ARBA:ARBA00022528, ECO:0000256|PIRNR:PIRNR000431};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098,
KW ECO:0000256|PIRNR:PIRNR000431};
KW Phospholipid biosynthesis {ECO:0000256|ARBA:ARBA00023209};
KW Phospholipid metabolism {ECO:0000256|ARBA:ARBA00023264};
KW Plastid {ECO:0000256|ARBA:ARBA00022640, ECO:0000256|PIRNR:PIRNR000431};
KW Reference proteome {ECO:0000313|Proteomes:UP000008827};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PIRNR:PIRNR000431};
KW Transit peptide {ECO:0000256|ARBA:ARBA00022946}.
FT DOMAIN 96..242
FT /note="Phospholipid/glycerol acyltransferase"
FT /evidence="ECO:0000259|SMART:SM00563"
FT MOTIF 102..107
FT /note="HXXXXD motif"
FT /evidence="ECO:0000256|PIRSR:PIRSR000431-2"
SQ SEQUENCE 332 AA; 37228 MW; AD26B39A41F91B8C CRC64;
MEEVYNNYKN AVIQSGDPKA KEIVLSNMIA LLDRVFLDVT DPFVFQPHHK AKREPFDYYV
FGQNYIRPLV DLKNSYVGNM PLFIEMEEKL KQGHNIILMS NHQTEADPAI IALLLETRIP
YIAESMTYVA GDRVITDPLS KPFSIGRNLI CVYSKKHMLD DPALIEMKRN ANIRALKEMA
MLLRSGSQIV WIAPSGGRDR PDPHTGEWAP APFDTSSVDN MRRLVEHSGP PGHVYPLAIL
CHDIMPPPLK VEKEIGEKRI ISFHGAGISV APALSFSETT ATSENPEKAK ELFSKALYDS
VTEQYNVLKS AIHGKKGFEA STPVVSLSQP WK
//
