ID K8GQX9_9CYAN Unreviewed; 787 AA.
AC K8GQX9;
DT 06-FEB-2013, integrated into UniProtKB/TrEMBL.
DT 06-FEB-2013, sequence version 1.
DT 27-MAR-2024, entry version 56.
DE RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU364053};
DE EC=5.6.2.4 {ECO:0000256|RuleBase:RU364053};
GN ORFNames=OsccyDRAFT_0374 {ECO:0000313|EMBL:EKQ70109.1};
OS Leptolyngbyaceae cyanobacterium JSC-12.
OC Bacteria; Cyanobacteriota; Cyanophyceae; Leptolyngbyales; Leptolyngbyaceae.
OX NCBI_TaxID=864702 {ECO:0000313|EMBL:EKQ70109.1, ECO:0000313|Proteomes:UP000001332};
RN [1] {ECO:0000313|EMBL:EKQ70109.1, ECO:0000313|Proteomes:UP000001332}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JSC-12 {ECO:0000313|EMBL:EKQ70109.1,
RC ECO:0000313|Proteomes:UP000001332};
RG DOE Joint Genome Institute;
RA Brown I., Huntemann M., Wei C.-L., Han J., Detter J.C., Han C., Tapia R.,
RA Chen A., Kyrpides N., Mavromatis K., Markowitz V., Szeto E., Ivanova N.,
RA Mikhailova N., Ovchinnikova G., Pagani I., Pati A., Goodwin L.,
RA Nordberg H.P., Cantor M.N., Hua S.X., Woyke T.;
RT "Improved high quality draft of Oscillatoriales sp. JSC-12.";
RL Submitted (OCT-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618,
CC ECO:0000256|RuleBase:RU364053};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922, ECO:0000256|RuleBase:RU364053}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EKQ70109.1}.
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DR EMBL; AJUB01000005; EKQ70109.1; -; Genomic_DNA.
DR AlphaFoldDB; K8GQX9; -.
DR STRING; 864702.OsccyDRAFT_0374; -.
DR PATRIC; fig|864702.5.peg.404; -.
DR eggNOG; COG0210; Bacteria.
DR HOGENOM; CLU_004585_5_2_3; -.
DR OrthoDB; 9810135at2; -.
DR Proteomes; UP000001332; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR005751; ATP-dep_DNA_helicase_PcrA.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR NCBIfam; TIGR01073; pcrA; 1.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA-binding {ECO:0000256|RuleBase:RU364053};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000001332}.
FT DOMAIN 12..345
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 346..622
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 33..40
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 787 AA; 89822 MW; 6A04FE9ADC6F4110 CRC64;
MTQTNQTKEF LSLLNASQRQ AVEHYSGPLL VVAGAGSGKT RALTFRIANL VLNHRVDPEN
ILAVTFTNKA AREMKERIEK LFAEQHARSE YDKPLEALSP EVQTRLKSQV YKSVTKDLWI
GTFHALCARI LRFDIEKYQD SHGRKWTRSF SIFDESDAQS LVKEIVTQKL NLDDKKFDPK
SMRYAISNAK NKCLTPEEVE KDQPNYRGRV LANIYSLYQD TLAANNALDF DDLILMPVQL
FRQNELVRTY WHNRFRHILV DEYQDTNRTQ YDFIRLLATN GEDSKTFDNW AHRSVFVVGD
ADQSIYSFRA ADFTILMDFQ TDFGDGLPDD DTRTMVKLEE NYRSTENILE VANQLIENNT
ERIDKVLKPT RSEGEAIYLY RADDETAEAE FVVNQIRQLE ARHPELHYGK FAILYRTNAQ
SRALEDSLVR HQIPYKVVGG LRFYDRKEIK DVLAYLRAIA NPFDTVSLLR VINTPRRGIG
KATIDNLVKA AQELNLPLWE ILTDETSVKT LAGRSSKGVL AFAEMLQKWR GELDNLPASE
IVQGVLEDSG YVKDLKEQGT EEATDRLGNV QELYNAVRQF EEENDDRSLI AFLANASLAS
DLDNLEEEET RVSLMTLHSS KGLEFPVVFL VGLEQGLFPN YRSMEDPAAL EEERRLCYVG
ITRAQERLFL SYARERRLYG NREPASPSLF LAELPKELLE SNVSSAIPTR YGKLIAESRR
SAPARAMSNI HENDWSIGDR ALHKAFGVGE VTRIFGAGNK ICLAVKFPGL GQKIIDPKIT
PIERVES
//
