ID K8PHY8_9BRAD Unreviewed; 695 AA.
AC K8PHY8;
DT 06-FEB-2013, integrated into UniProtKB/TrEMBL.
DT 06-FEB-2013, sequence version 1.
DT 27-MAR-2024, entry version 52.
DE SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:EKS41156.1};
GN ORFNames=HMPREF9695_00248 {ECO:0000313|EMBL:EKS41156.1};
OS Afipia broomeae ATCC 49717.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Nitrobacteraceae; Afipia.
OX NCBI_TaxID=883078 {ECO:0000313|EMBL:EKS41156.1, ECO:0000313|Proteomes:UP000001096};
RN [1] {ECO:0000313|EMBL:EKS41156.1, ECO:0000313|Proteomes:UP000001096}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49717 {ECO:0000313|EMBL:EKS41156.1,
RC ECO:0000313|Proteomes:UP000001096};
RG The Broad Institute Genome Sequencing Platform;
RA Earl A., Ward D., Feldgarden M., Gevers D., Huys G., Walker B., Young S.K.,
RA Zeng Q., Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Alvarado L.,
RA Arachchi H.M., Berlin A., Chapman S.B., Goldberg J., Griggs A., Gujja S.,
RA Hansen M., Howarth C., Imamovic A., Larimer J., McCowen C., Montmayeur A.,
RA Murphy C., Neiman D., Pearson M., Priest M., Roberts A., Saif S., Shea T.,
RA Sisk P., Sykes S., Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Afipia broomeae ATCC 49717.";
RL Submitted (APR-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) +
CC NADH; Xref=Rhea:RHEA:22432, ChEBI:CHEBI:15378, ChEBI:CHEBI:57318,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:90726; EC=1.1.1.35;
CC Evidence={ECO:0000256|ARBA:ARBA00023693};
CC -!- PATHWAY: Lipid metabolism; fatty acid beta-oxidation.
CC {ECO:0000256|ARBA:ARBA00005005}.
CC -!- SIMILARITY: In the central section; belongs to the 3-hydroxyacyl-CoA
CC dehydrogenase family. {ECO:0000256|ARBA:ARBA00007005}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EKS41156.1}.
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DR EMBL; AGWX01000001; EKS41156.1; -; Genomic_DNA.
DR RefSeq; WP_006018950.1; NZ_KB375282.1.
DR AlphaFoldDB; K8PHY8; -.
DR PATRIC; fig|883078.3.peg.258; -.
DR eggNOG; COG1024; Bacteria.
DR eggNOG; COG1250; Bacteria.
DR HOGENOM; CLU_009834_16_3_5; -.
DR UniPathway; UPA00659; -.
DR Proteomes; UP000001096; Unassembled WGS sequence.
DR GO; GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0070403; F:NAD+ binding; IEA:InterPro.
DR GO; GO:0006635; P:fatty acid beta-oxidation; IEA:UniProtKB-UniPathway.
DR CDD; cd06558; crotonase-like; 1.
DR Gene3D; 1.10.1040.50; -; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
DR InterPro; IPR006108; 3HC_DH_C.
DR InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR43612; TRIFUNCTIONAL ENZYME SUBUNIT ALPHA; 1.
DR PANTHER; PTHR43612:SF3; TRIFUNCTIONAL ENZYME SUBUNIT ALPHA, MITOCHONDRIAL; 1.
DR Pfam; PF00725; 3HCDH; 1.
DR Pfam; PF02737; 3HCDH_N; 1.
DR Pfam; PF00378; ECH_1; 1.
DR SUPFAM; SSF48179; 6-phosphogluconate dehydrogenase C-terminal domain-like; 2.
DR SUPFAM; SSF52096; ClpP/crotonase; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW Fatty acid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Lipid degradation {ECO:0000256|ARBA:ARBA00022963};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Lyase {ECO:0000256|ARBA:ARBA00023239};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Reference proteome {ECO:0000313|Proteomes:UP000001096}.
FT DOMAIN 324..497
FT /note="3-hydroxyacyl-CoA dehydrogenase NAD binding"
FT /evidence="ECO:0000259|Pfam:PF02737"
FT DOMAIN 501..596
FT /note="3-hydroxyacyl-CoA dehydrogenase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF00725"
SQ SEQUENCE 695 AA; 75239 MW; 0F3CD384123B0F28 CRC64;
MDSRIMDVLK DRVLELGPEP QAGPYRNFKL TRDSDGIAWL LFDREGTSAN TLSASVLEEF
ETVIAALEQD RPAGIVIRSA KPSGFIAGAD VNEFRGVTDP REVESQMARA HAVVDRLEAL
KVPSVAVIHG FCLGGGLEVA LACNTRIAID NARFGFPEVM LGLHPGLGGT ARFTHLVSPL
EAMTMMLTGK TIDARKAKSL GLADAVTQER HVRNAVKDAI FGRLRPHRQG LLARAMNLGP
VRGLLANRMR GEAAKTAPKD HYPAPYALIG LWEKHGGDRK AMLAAEQVSF ANLMVTPTAQ
NLIRVFFLRE QMKKLADGRN TVKHVHVIGA GAMGGDIAAW CAYQGLRVTL ADMKAEPIAG
AVKRASDLFG KIIRKKADLR DALDRLMPDM TGEGVRNADL IIEAVPEKLE LKQKVYAGLE
PVMKAGAILA TNTSSIPLQD LRTTLTHPER LLGLHFFNPV SRLQLVEVVS HDTVDPHALA
TALKFVGAID RLPLPVKSSP GFLVNRALTP YMLEAMVMLD EKVDKAVIDA AAEKFGMPMG
PIELADQVGL DICLAVGDML RSKFGEAALP PAPDWLRDKV KKGELGRKTG KGFYEWKDGK
AVKLPSPVQP TEEMIDRLIL PMSNVCVACL REGIVENGDV VDGAMIFGTG YAPFRGGPLN
YARTRGAANV VAALKALEAK FGGRFKPDAG WDTFA
//
