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Database: UniProt
Entry: K8WIA3_9GAMM
LinkDB: K8WIA3_9GAMM
Original site: K8WIA3_9GAMM 
ID   K8WIA3_9GAMM            Unreviewed;        84 AA.
AC   K8WIA3;
DT   06-FEB-2013, integrated into UniProtKB/TrEMBL.
DT   06-FEB-2013, sequence version 1.
DT   25-OCT-2017, entry version 32.
DE   RecName: Full=Sulfur carrier protein TusA {ECO:0000256|HAMAP-Rule:MF_00413, ECO:0000256|SAAS:SAAS00728055};
DE   AltName: Full=Sulfur mediator TusA {ECO:0000256|HAMAP-Rule:MF_00413};
DE   AltName: Full=Sulfur transfer protein TusA {ECO:0000256|HAMAP-Rule:MF_00413};
DE   AltName: Full=tRNA 2-thiouridine synthesizing protein A {ECO:0000256|HAMAP-Rule:MF_00413};
GN   Name=tusA {ECO:0000256|HAMAP-Rule:MF_00413};
GN   ORFNames=OOA_12840 {ECO:0000313|EMBL:EKT60303.1};
OS   Providencia burhodogranariea DSM 19968.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Morganellaceae; Providencia.
OX   NCBI_TaxID=1141662 {ECO:0000313|EMBL:EKT60303.1, ECO:0000313|Proteomes:UP000009336};
RN   [1] {ECO:0000313|EMBL:EKT60303.1, ECO:0000313|Proteomes:UP000009336}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 19968 {ECO:0000313|EMBL:EKT60303.1,
RC   ECO:0000313|Proteomes:UP000009336};
RX   PubMed=23145767; DOI=10.1186/1471-2164-13-612;
RA   Galac M.R., Lazzaro B.P.;
RT   "Comparative genomics of bacteria in the genus Providencia isolated
RT   from wild Drosophila melanogaster.";
RL   BMC Genomics 13:612-612(2012).
CC   -!- FUNCTION: Sulfur carrier protein involved in sulfur trafficking in
CC       the cell. Part of a sulfur-relay system required for 2-thiolation
CC       during synthesis of 2-thiouridine of the modified wobble base 5-
CC       methylaminomethyl-2-thiouridine (mnm(5)s(2)U) in tRNA. Interacts
CC       with IscS and stimulates its cysteine desulfurase activity.
CC       Accepts an activated sulfur from IscS, which is then transferred
CC       to TusD, and thus determines the direction of sulfur flow from
CC       IscS to 2-thiouridine formation. Also appears to be involved in
CC       sulfur transfer for the biosynthesis of molybdopterin.
CC       {ECO:0000256|HAMAP-Rule:MF_00413}.
CC   -!- PATHWAY: tRNA modification. {ECO:0000256|HAMAP-Rule:MF_00413}.
CC   -!- SUBUNIT: Interacts with IscS. {ECO:0000256|HAMAP-Rule:MF_00413}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00413,
CC       ECO:0000256|SAAS:SAAS00634305}.
CC   -!- SIMILARITY: Belongs to the sulfur carrier protein TusA family.
CC       {ECO:0000256|HAMAP-Rule:MF_00413, ECO:0000256|SAAS:SAAS00784432}.
CC   -!- CAUTION: The sequence shown here is derived from an
CC       EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is
CC       preliminary data. {ECO:0000313|EMBL:EKT60303.1}.
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DR   EMBL; AKKL01000034; EKT60303.1; -; Genomic_DNA.
DR   RefSeq; WP_008912562.1; NZ_KB233223.1.
DR   EnsemblBacteria; EKT60303; EKT60303; OOA_12840.
DR   PATRIC; fig|1141662.3.peg.2610; -.
DR   OrthoDB; POG091H06BL; -.
DR   Proteomes; UP000009336; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0097163; F:sulfur carrier activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:InterPro.
DR   CDD; cd03423; SirA; 1.
DR   Gene3D; 3.30.110.40; -; 1.
DR   HAMAP; MF_00413; Thiourid_synth_A; 1.
DR   InterPro; IPR022931; Sulphur_carrier_TusA.
DR   InterPro; IPR001455; TusA-like.
DR   InterPro; IPR036868; TusA-like_sf.
DR   Pfam; PF01206; TusA; 1.
DR   SUPFAM; SSF64307; SSF64307; 1.
DR   PROSITE; PS01148; UPF0033; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000009336};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00413,
KW   ECO:0000256|SAAS:SAAS00634304};
KW   Reference proteome {ECO:0000313|Proteomes:UP000009336};
KW   tRNA processing {ECO:0000256|HAMAP-Rule:MF_00413}.
FT   DOMAIN       12     36       UPF0033. {ECO:0000259|PROSITE:PS01148}.
FT   ACT_SITE     19     19       Cysteine persulfide intermediate.
FT                                {ECO:0000256|HAMAP-Rule:MF_00413}.
SQ   SEQUENCE   84 AA;  9327 MW;  B992BA9E77B2D8CD CRC64;
     MSDLFSNATK TLDTLGLRCP EPVMLVRKTV RGMNAGDTLL IIADDPATTR DIPGFCRFME
     HELLAAEAEQ APYRYLLRKK SAGL
//
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