ID K9CVF2_9FIRM Unreviewed; 815 AA.
AC K9CVF2;
DT 06-FEB-2013, integrated into UniProtKB/TrEMBL.
DT 06-FEB-2013, sequence version 1.
DT 27-MAR-2024, entry version 48.
DE SubName: Full=CRISPR-associated helicase cas3 {ECO:0000313|EMBL:EKU70787.1};
GN ORFNames=HMPREF9161_01833 {ECO:0000313|EMBL:EKU70787.1};
OS Selenomonas sp. F0473.
OC Bacteria; Bacillota; Negativicutes; Selenomonadales; Selenomonadaceae;
OC Selenomonas.
OX NCBI_TaxID=999423 {ECO:0000313|EMBL:EKU70787.1, ECO:0000313|Proteomes:UP000009883};
RN [1] {ECO:0000313|EMBL:EKU70787.1, ECO:0000313|Proteomes:UP000009883}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=F0473 {ECO:0000313|EMBL:EKU70787.1,
RC ECO:0000313|Proteomes:UP000009883};
RG The Broad Institute Genome Sequencing Platform;
RA Earl A., Ward D., Feldgarden M., Gevers D., Blanton J.M., Baranova O.V.,
RA Mathney J., Dewhirst F.E., Izard J., Tanner A.C., Walker B., Young S.K.,
RA Zeng Q., Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Alvarado L.,
RA Arachchi H.M., Berlin A., Chapman S.B., Goldberg J., Griggs A., Gujja S.,
RA Hansen M., Howarth C., Imamovic A., Larimer J., McCowen C., Montmayeur A.,
RA Murphy C., Neiman D., Pearson M., Priest M., Roberts A., Saif S., Shea T.,
RA Sisk P., Sykes S., Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Selenomonas sp. Oral taxon 133 strain F0473.";
RL Submitted (SEP-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: In the N-terminal section; belongs to the CRISPR-associated
CC nuclease Cas3-HD family. {ECO:0000256|ARBA:ARBA00006847}.
CC -!- SIMILARITY: In the central section; belongs to the CRISPR-associated
CC helicase Cas3 family. {ECO:0000256|ARBA:ARBA00009046}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EKU70787.1}.
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DR EMBL; AGZT01000008; EKU70787.1; -; Genomic_DNA.
DR RefSeq; WP_009729417.1; NZ_JH992902.1.
DR AlphaFoldDB; K9CVF2; -.
DR STRING; 999423.HMPREF9161_01833; -.
DR PATRIC; fig|999423.3.peg.1932; -.
DR eggNOG; COG1203; Bacteria.
DR HOGENOM; CLU_010123_0_0_9; -.
DR OrthoDB; 9810236at2; -.
DR Proteomes; UP000009883; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR CDD; cd09641; Cas3''_I; 1.
DR CDD; cd17930; DEXHc_cas3; 1.
DR Gene3D; 1.10.3210.30; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR006483; CRISPR-assoc_Cas3_HD.
DR InterPro; IPR038257; CRISPR-assoc_Cas3_HD_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR006474; Helicase_Cas3_CRISPR-ass_core.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR01587; cas3_core; 1.
DR NCBIfam; TIGR01596; cas3_HD; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SUPFAM; SSF109604; HD-domain/PDEase-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51643; HD_CAS3; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
PE 3: Inferred from homology;
KW Antiviral defense {ECO:0000256|ARBA:ARBA00023118};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000009883}.
FT DOMAIN 13..216
FT /note="HD Cas3-type"
FT /evidence="ECO:0000259|PROSITE:PS51643"
FT DOMAIN 275..462
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT COILED 165..192
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 815 AA; 92244 MW; C0BBD7FF9E1B30AF CRC64;
MTKPYLAHIR RNSSGIEQTI YAHLHRVADL MSASAAKMGL SSTARLIGIL HDLGKCSEAF
ADYLSWSHAH PGDYSRRGTV DHSSAGGQLL MQRYGGKSDD KQLVIEIAAL AIFSHHTGLL
NYIGEHGLMN ASGREDAYTF SRRMDKADIL TGVNLGYFFE NIISEEELDN LFAEAVHELS
ALEEQIQKQK SDEEYFFYWG MVVKILFSML VDADRLDSAE FELDDSLTCA WDTESLWEEF
SAKLEKKIVG FPISREEKAK KIATLRQRIS DDCLHAAEQT PGIYRLTVPT GGGKTLASMR
FALAHAKRRH KKRIIVVIPY TSIIDQNAKE IREIFDRDEA ILEHHSNVVS EEDNGEDTEC
MDWRRILTER WDVPVIFTTQ MQFLNTLFSG GNSSIRRLHA LQDSILIFDE IQTLPVKCTY
LFNLAINFLK DFCRTTTVLC TATQPPLEQL DFPIGAGKNA ELTTDISETF EAFRRVHIEN
CCRDGGSSVE DIAAAICRSA EEEGDVLCIV NLTKQARELY QAVSEIAQDS DIEIRTVHLS
TKMCPAHRKK VLAEVREELR CRKEHPERRL ICISTQLVEA GVDVSFPVVY RALAGFSSIA
QAAGRCNRHG ELAQGIVRIF AFTDENLSRL EDIQAGQKIA RVLLFEKEAD VLLSPQVMDE
YFRKFYKEQI ESRMRYPLHD GNTLLDLLSV NRAGVGVCNQ RGDKLNTGFA HAFHDAGRAF
EVIDSHAEAV LVPYGAGKEL LLAFDAKSED KRLFYRNMKR AQQYMVNLFS YEIKRFSGMG
AVWRTESGVI ALREEYYDKA LGVQFEEQQN AYCMI
//
