ID K9JZI4_ONOVI Unreviewed; 389 AA.
AC K9JZI4;
DT 06-MAR-2013, integrated into UniProtKB/TrEMBL.
DT 06-MAR-2013, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=chalcone synthase {ECO:0000256|ARBA:ARBA00012975};
DE EC=2.3.1.74 {ECO:0000256|ARBA:ARBA00012975};
OS Onobrychis viciifolia (Common sainfoin).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Hedysareae; Onobrychis.
OX NCBI_TaxID=3882 {ECO:0000313|EMBL:AEF14414.1};
RN [1] {ECO:0000313|EMBL:AEF14414.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=22818525; DOI=10.1016/j.phytochem.2012.05.030;
RA Thill J., Regos I., Farag M.A., Ahmad A.F., Kusek J., Castro A.,
RA Schlangen K., Carbonero C.H., Gadjev I.Z., Smith L.M., Halbwirth H.,
RA Treutter D., Stich K.;
RT "Polyphenol metabolism provides a screening tool for beneficial effects of
RT Onobrychis viciifolia (sainfoin).";
RL Phytochemistry 82:67-80(2012).
CC -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC which can under specific conditions spontaneously isomerize into
CC naringenin. {ECO:0000256|ARBA:ARBA00002969}.
CC -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004966}.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000256|ARBA:ARBA00005531,
CC ECO:0000256|RuleBase:RU003633}.
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DR EMBL; HM204482; AEF14414.1; -; mRNA.
DR AlphaFoldDB; K9JZI4; -.
DR UniPathway; UPA00154; -.
DR GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00831; CHS_like; 1.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR InterPro; IPR011141; Polyketide_synthase_type-III.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR11877:SF14; CHALCONE SYNTHASE; 1.
DR PANTHER; PTHR11877; HYDROXYMETHYLGLUTARYL-COA SYNTHASE; 1.
DR Pfam; PF02797; Chal_sti_synt_C; 1.
DR Pfam; PF00195; Chal_sti_synt_N; 1.
DR PIRSF; PIRSF000451; PKS_III; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
PE 2: Evidence at transcript level;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315,
KW ECO:0000256|RuleBase:RU003633};
KW Flavonoid biosynthesis {ECO:0000256|ARBA:ARBA00023241};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU003633}.
FT DOMAIN 6..228
FT /note="Chalcone/stilbene synthase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00195"
FT DOMAIN 238..387
FT /note="Chalcone/stilbene synthase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02797"
FT ACT_SITE 164
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000451-1"
SQ SEQUENCE 389 AA; 42611 MW; 4017DB15F268E987 CRC64;
MVSVAEIRKA QRAEGPATVL AIGTATPSNC VVQSEYPDFY FRITNSEHKT ELKEKFKRMC
DKSMIKKRYM HLTEEILKEN PSMCEYMAPS LDARQDMVVV EVPKLGKEAA TKAIKEWGQP
KSKITHLIFC TTSGVDMPGA DYQLTKLLGL RPSVKRYMMY QQGCLAGGTV LRLAKDLAEN
NKGARVLVVC SEITAVTFRG PTDTHLDSLV GQALFGDGAA AVIVGSDPLP EIEKPLFELV
WTAQTILPDS EGAIDGHLRE VGLTFHLLKD VPGLISKNIE KALVEAFQPL GISDYNSIFW
IAHPGGPAIL DQVEVKLGLK PEKMQATRHV LSEYGNMSSA CVLFILDEMR RKSKENGLAT
TGEGLEWGVL FGFGPGLTVE TVVLRSVAA
//
