ID K9NK82_9PSED Unreviewed; 475 AA.
AC K9NK82;
DT 06-MAR-2013, integrated into UniProtKB/TrEMBL.
DT 06-MAR-2013, sequence version 1.
DT 24-JAN-2024, entry version 39.
DE RecName: Full=Small-conductance mechanosensitive channel {ECO:0000256|RuleBase:RU369025};
GN ORFNames=PputUW4_03301 {ECO:0000313|EMBL:AFY20496.1};
OS Pseudomonas sp. UW4.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=1207075 {ECO:0000313|EMBL:AFY20496.1, ECO:0000313|Proteomes:UP000010397};
RN [1] {ECO:0000313|EMBL:AFY20496.1, ECO:0000313|Proteomes:UP000010397}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UW4 {ECO:0000313|EMBL:AFY20496.1,
RC ECO:0000313|Proteomes:UP000010397};
RX PubMed=23516524;
RA Duan J., Jiang W., Cheng Z., Heikkila J.J., Glick B.R.;
RT "The Complete Genome Sequence of the Plant Growth-Promoting Bacterium
RT Pseudomonas sp. UW4.";
RL PLoS ONE 8:E58640-E58640(2013).
CC -!- FUNCTION: Mechanosensitive channel that participates in the regulation
CC of osmotic pressure changes within the cell, opening in response to
CC stretch forces in the membrane lipid bilayer, without the need for
CC other proteins. Contributes to normal resistance to hypoosmotic shock.
CC Forms an ion channel of 1.0 nanosiemens conductance with a slight
CC preference for anions. {ECO:0000256|RuleBase:RU369025}.
CC -!- SUBUNIT: Homoheptamer. {ECO:0000256|RuleBase:RU369025}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000256|RuleBase:RU369025}; Multi-pass membrane protein
CC {ECO:0000256|RuleBase:RU369025}.
CC -!- SIMILARITY: Belongs to the MscS (TC 1.A.23) family.
CC {ECO:0000256|RuleBase:RU369025}.
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DR EMBL; CP003880; AFY20496.1; -; Genomic_DNA.
DR RefSeq; WP_015095764.1; NC_019670.1.
DR AlphaFoldDB; K9NK82; -.
DR STRING; 1207075.PputUW4_03301; -.
DR KEGG; ppuu:PputUW4_03301; -.
DR PATRIC; fig|1207075.3.peg.3369; -.
DR eggNOG; COG0668; Bacteria.
DR eggNOG; COG2905; Bacteria.
DR HOGENOM; CLU_032479_1_0_6; -.
DR OrthoDB; 9775207at2; -.
DR Proteomes; UP000010397; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008381; F:mechanosensitive monoatomic ion channel activity; IEA:InterPro.
DR CDD; cd00038; CAP_ED; 1.
DR Gene3D; 1.10.287.1260; -; 1.
DR Gene3D; 2.30.30.60; -; 1.
DR Gene3D; 2.60.120.10; Jelly Rolls; 1.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR018490; cNMP-bd_dom_sf.
DR InterPro; IPR016846; cNMP-bd_ion_channel.
DR InterPro; IPR010920; LSM_dom_sf.
DR InterPro; IPR045275; MscS_archaea/bacteria_type.
DR InterPro; IPR023408; MscS_beta-dom_sf.
DR InterPro; IPR006685; MscS_channel_2nd.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR PANTHER; PTHR30221; SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL; 1.
DR PANTHER; PTHR30221:SF1; SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL; 1.
DR Pfam; PF00027; cNMP_binding; 1.
DR Pfam; PF00924; MS_channel_2nd; 1.
DR PIRSF; PIRSF026673; UCP026673_ion_chan; 1.
DR SMART; SM00100; cNMP; 1.
DR SUPFAM; SSF51206; cAMP-binding domain-like; 1.
DR SUPFAM; SSF50182; Sm-like ribonucleoproteins; 1.
DR PROSITE; PS50042; CNMP_BINDING_3; 1.
PE 3: Inferred from homology;
KW Cell inner membrane {ECO:0000256|RuleBase:RU369025};
KW Cell membrane {ECO:0000256|RuleBase:RU369025};
KW Ion channel {ECO:0000256|RuleBase:RU369025};
KW Ion transport {ECO:0000256|RuleBase:RU369025};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU369025};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|RuleBase:RU369025};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|RuleBase:RU369025}; Transport {ECO:0000256|RuleBase:RU369025}.
FT TRANSMEM 6..25
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU369025"
FT TRANSMEM 37..57
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU369025"
FT TRANSMEM 77..96
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU369025"
FT TRANSMEM 108..127
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU369025"
FT DOMAIN 329..439
FT /note="Cyclic nucleotide-binding"
FT /evidence="ECO:0000259|PROSITE:PS50042"
SQ SEQUENCE 475 AA; 51257 MW; B0E279AEFB48051B CRC64;
MSYIFSHLLS LSSLLLAIDA LLWHLAPFKH RVSRVGVRLA LFLLFSAVLI NAGVSPLQAP
LFADDRVAQL GATALGILWW LYAARVLTEV IGLALMRRIG HSGRLLQDVI GALVFLIAVV
AAAGYVLELP VKGLLATSGV VAIVVGLALQ STLSDVFSGI VLNTTKPYQV DDRVSIDGIE
GKVLDIDWRA THLLTSAGST AVVPNSVAAK AKIVNLSRPF NMHGVSISIQ VPNHIRPRRV
LDALDRTLQG SSSLLLDPAP KAVLKEAGET MSEYVASGFI AELGKKGEVR NQLFDLAHRH
LEAAGISRQP DGVIEPSTRA RALLDEVKIF RSLSAEERDR LAQNMVAQQY AAGDVVLDLE
EVPDSLFVIA TGVVSATVPD GSTQIEAGRM GPSEVMGEQS ILADTPSQAR FTAMTSSIIY
RLDKSLTRDC MGQRREVDRA LNKLQAVRQQ NSRMALMAKP VAVRKGGFLG WLQSR
//
