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Entry: KANU_STAAU
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ID   KANU_STAAU              Reviewed;         253 AA.
AC   P05057;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   13-AUG-1987, sequence version 1.
DT   08-NOV-2023, entry version 107.
DE   RecName: Full=Kanamycin nucleotidyltransferase;
DE            Short=Neo(R);
DE            EC=2.7.7.-;
GN   Name=knt; Synonyms=kan;
OS   Staphylococcus aureus.
OG   Plasmid pUB110.
OC   Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3007933; DOI=10.1007/bf00330534;
RA   Mueller R.E., Ano T., Imanaka T., Aiba S.;
RT   "Complete nucleotide sequences of Bacillus plasmids pUB110dB, pRBH1 and its
RT   copy mutants.";
RL   Mol. Gen. Genet. 202:169-171(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-7.
RX   PubMed=6090428; DOI=10.1128/jb.160.1.413-420.1984;
RA   Matsumura M., Katakura Y., Imanaka T., Aiba S.;
RT   "Enzymatic and nucleotide sequence studies of a kanamycin-inactivating
RT   enzyme encoded by a plasmid from thermophilic bacilli in comparison with
RT   that encoded by plasmid pUB110.";
RL   J. Bacteriol. 160:413-420(1984).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3744038;
RA   Bashkirov V.I., Mil'Shina N.V., Prozorov A.A.;
RT   "Nucleotide sequence and physical map of kanamycin-resistant plasmid pUB110
RT   from Staphylococcus aureus.";
RL   Genetika 22:1081-1092(1986).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3010356; DOI=10.1016/0147-619x(86)90046-6;
RA   McKenzie T., Hoshino T., Tanaka T., Sueoka N.;
RT   "The nucleotide sequence of pUB110: some salient features in relation to
RT   replication and its regulation.";
RL   Plasmid 15:93-103(1986).
RN   [5]
RP   SEQUENCE REVISION.
RX   PubMed=3033723; DOI=10.1016/0147-619x(87)90015-1;
RA   McKenzie T., Hoshino T., Tanaka T., Sueoka N.;
RT   "Correction. A revision of the nucleotide sequence and functional map of
RT   pUB110.";
RL   Plasmid 17:83-85(1987).
RN   [6]
RP   X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
RX   PubMed=8218273; DOI=10.1021/bi00096a006;
RA   Sakon J., Liao H.H., Kanikula A.M., Benning M.M., Rayment I., Holden H.M.;
RT   "Molecular structure of kanamycin nucleotidyltransferase determined to 3.0-
RT   A resolution.";
RL   Biochemistry 32:11977-11984(1993).
RN   [7]
RP   X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
RX   PubMed=7577914; DOI=10.1021/bi00041a005;
RA   Pedersen L.C., Benning M.M., Holden H.M.;
RT   "Structural investigation of the antibiotic and ATP-binding sites in
RT   kanamycin nucleotidyltransferase.";
RL   Biochemistry 34:13305-13311(1995).
CC   -!- FUNCTION: Inactivates the antibiotic kanamycin by catalyzing the
CC       transfer of a nucleotidyl group from nucleoside triphosphates such as
CC       ATP to the 4'-hydroxyl group of the aminoglycoside.
CC   -!- SUBUNIT: Homodimer.
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DR   EMBL; X03408; CAA27142.1; -; Genomic_DNA.
DR   EMBL; M37273; AAA98214.1; -; Genomic_DNA.
DR   EMBL; M19465; AAA88361.1; -; Genomic_DNA.
DR   RefSeq; NP_040433.1; NC_001384.1.
DR   RefSeq; WP_001014230.1; NG_047373.1.
DR   RefSeq; YP_006937662.1; NC_013320.1.
DR   RefSeq; YP_006938491.1; NC_013342.1.
DR   PDB; 1KAN; X-ray; 3.00 A; A/B=1-253.
DR   PDB; 1KNY; X-ray; 2.50 A; A/B=1-253.
DR   PDB; 6NLT; X-ray; 1.90 A; A/B=1-253.
DR   PDB; 6UN8; X-ray; 1.65 A; A/B=1-253.
DR   PDBsum; 1KAN; -.
DR   PDBsum; 1KNY; -.
DR   PDBsum; 6NLT; -.
DR   PDBsum; 6UN8; -.
DR   AlphaFoldDB; P05057; -.
DR   SMR; P05057; -.
DR   DrugBank; DB01172; Kanamycin.
DR   KEGG; ag:AAA88361; -.
DR   EvolutionaryTrace; P05057; -.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   CDD; cd05397; NT_Pol-beta-like; 1.
DR   Gene3D; 3.30.460.10; Beta Polymerase, domain 2; 1.
DR   Gene3D; 1.20.120.330; Nucleotidyltransferases domain 2; 1.
DR   InterPro; IPR012481; KNTase_C.
DR   InterPro; IPR043519; NT_sf.
DR   Pfam; PF07827; KNTase_C; 1.
DR   SUPFAM; SSF81301; Nucleotidyltransferase; 1.
DR   SUPFAM; SSF81593; Nucleotidyltransferase substrate binding subunit/domain; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antibiotic resistance; Direct protein sequencing; Plasmid;
KW   Transferase.
FT   CHAIN           1..253
FT                   /note="Kanamycin nucleotidyltransferase"
FT                   /id="PRO_0000068568"
FT   HELIX           9..27
FT                   /evidence="ECO:0007829|PDB:6UN8"
FT   HELIX           28..30
FT                   /evidence="ECO:0007829|PDB:6UN8"
FT   STRAND          31..37
FT                   /evidence="ECO:0007829|PDB:6UN8"
FT   HELIX           38..41
FT                   /evidence="ECO:0007829|PDB:6UN8"
FT   STRAND          51..59
FT                   /evidence="ECO:0007829|PDB:6UN8"
FT   STRAND          63..69
FT                   /evidence="ECO:0007829|PDB:6UN8"
FT   STRAND          74..81
FT                   /evidence="ECO:0007829|PDB:6UN8"
FT   HELIX           82..90
FT                   /evidence="ECO:0007829|PDB:6UN8"
FT   HELIX           96..99
FT                   /evidence="ECO:0007829|PDB:6UN8"
FT   HELIX           100..104
FT                   /evidence="ECO:0007829|PDB:6UN8"
FT   STRAND          107..114
FT                   /evidence="ECO:0007829|PDB:6UN8"
FT   HELIX           115..124
FT                   /evidence="ECO:0007829|PDB:6UN8"
FT   HELIX           128..141
FT                   /evidence="ECO:0007829|PDB:6UN8"
FT   HELIX           143..156
FT                   /evidence="ECO:0007829|PDB:6UN8"
FT   HELIX           159..161
FT                   /evidence="ECO:0007829|PDB:6UN8"
FT   HELIX           162..181
FT                   /evidence="ECO:0007829|PDB:6UN8"
FT   HELIX           188..190
FT                   /evidence="ECO:0007829|PDB:6UN8"
FT   HELIX           191..196
FT                   /evidence="ECO:0007829|PDB:6UN8"
FT   STRAND          198..200
FT                   /evidence="ECO:0007829|PDB:6UN8"
FT   HELIX           205..214
FT                   /evidence="ECO:0007829|PDB:6UN8"
FT   HELIX           220..241
FT                   /evidence="ECO:0007829|PDB:6UN8"
SQ   SEQUENCE   253 AA;  28798 MW;  9CB3D60E72A45DC6 CRC64;
     MNGPIIMTRE ERMKIVHEIK ERILDKYGDD VKAIGVYGSL GRQTDGPYSD IEMMCVMSTE
     EAEFSHEWTT GEWKVEVNFD SEEILLDYAS QVESDWPLTH GQFFSILPIY DSGGYLEKVY
     QTAKSVEAQT FHDAICALIV EELFEYAGKW RNIRVQGPTT FLPSLTVQVA MAGAMLIGLH
     HRICYTTSAS VLTEAVKQSD LPSGYDHLCQ FVMSGQLSDS EKLLESLENF WNGIQEWTER
     HGYIVDVSKR IPF
//
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