ID KCY_THET8 Reviewed; 208 AA.
AC Q5SL35;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 27-MAR-2024, entry version 99.
DE RecName: Full=Cytidylate kinase {ECO:0000255|HAMAP-Rule:MF_00238};
DE Short=CK {ECO:0000255|HAMAP-Rule:MF_00238};
DE EC=2.7.4.25 {ECO:0000255|HAMAP-Rule:MF_00238};
DE AltName: Full=Cytidine monophosphate kinase {ECO:0000255|HAMAP-Rule:MF_00238};
DE Short=CMP kinase {ECO:0000255|HAMAP-Rule:MF_00238};
GN Name=cmk {ECO:0000255|HAMAP-Rule:MF_00238}; OrderedLocusNames=TTHA0458;
OS Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
OC Bacteria; Deinococcota; Deinococci; Thermales; Thermaceae; Thermus.
OX NCBI_TaxID=300852;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27634 / DSM 579 / HB8;
RA Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T.,
RA Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.;
RT "Complete genome sequence of Thermus thermophilus HB8.";
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + CMP = ADP + CDP; Xref=Rhea:RHEA:11600,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:58069, ChEBI:CHEBI:60377,
CC ChEBI:CHEBI:456216; EC=2.7.4.25; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00238};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + dCMP = ADP + dCDP; Xref=Rhea:RHEA:25094,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:57566, ChEBI:CHEBI:58593,
CC ChEBI:CHEBI:456216; EC=2.7.4.25; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00238};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00238}.
CC -!- SIMILARITY: Belongs to the cytidylate kinase family. Type 1 subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00238}.
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DR EMBL; AP008226; BAD70281.1; -; Genomic_DNA.
DR RefSeq; WP_011227951.1; NC_006461.1.
DR RefSeq; YP_143724.1; NC_006461.1.
DR PDB; 3AKC; X-ray; 1.65 A; A=1-208.
DR PDB; 3AKD; X-ray; 1.60 A; A=1-208.
DR PDB; 3W8N; X-ray; 2.20 A; A=1-208.
DR PDB; 3W90; X-ray; 1.65 A; A=1-208.
DR PDB; 7CKJ; X-ray; 1.50 A; A=1-208.
DR PDBsum; 3AKC; -.
DR PDBsum; 3AKD; -.
DR PDBsum; 3W8N; -.
DR PDBsum; 3W90; -.
DR PDBsum; 7CKJ; -.
DR AlphaFoldDB; Q5SL35; -.
DR SMR; Q5SL35; -.
DR EnsemblBacteria; BAD70281; BAD70281; BAD70281.
DR GeneID; 3170130; -.
DR KEGG; ttj:TTHA0458; -.
DR PATRIC; fig|300852.9.peg.457; -.
DR eggNOG; COG0283; Bacteria.
DR HOGENOM; CLU_079959_0_0_0; -.
DR PhylomeDB; Q5SL35; -.
DR Proteomes; UP000000532; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0036430; F:CMP kinase activity; IEA:RHEA.
DR GO; GO:0036431; F:dCMP kinase activity; IEA:RHEA.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0006220; P:pyrimidine nucleotide metabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd02020; CMPK; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR HAMAP; MF_00238; Cytidyl_kinase_type1; 1.
DR InterPro; IPR003136; Cytidylate_kin.
DR InterPro; IPR011994; Cytidylate_kinase_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR00017; cmk; 1.
DR PANTHER; PTHR21299:SF2; CYTIDYLATE KINASE; 1.
DR PANTHER; PTHR21299; CYTIDYLATE KINASE/PANTOATE-BETA-ALANINE LIGASE; 1.
DR Pfam; PF02224; Cytidylate_kin; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Cytoplasm; Kinase; Nucleotide-binding;
KW Reference proteome; Transferase.
FT CHAIN 1..208
FT /note="Cytidylate kinase"
FT /id="PRO_1000048310"
FT BINDING 9..17
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00238"
FT STRAND 3..8
FT /evidence="ECO:0007829|PDB:7CKJ"
FT HELIX 15..26
FT /evidence="ECO:0007829|PDB:7CKJ"
FT STRAND 30..32
FT /evidence="ECO:0007829|PDB:7CKJ"
FT HELIX 33..47
FT /evidence="ECO:0007829|PDB:7CKJ"
FT HELIX 54..63
FT /evidence="ECO:0007829|PDB:7CKJ"
FT STRAND 67..69
FT /evidence="ECO:0007829|PDB:7CKJ"
FT STRAND 76..79
FT /evidence="ECO:0007829|PDB:7CKJ"
FT HELIX 85..87
FT /evidence="ECO:0007829|PDB:7CKJ"
FT STRAND 88..90
FT /evidence="ECO:0007829|PDB:7CKJ"
FT HELIX 91..101
FT /evidence="ECO:0007829|PDB:7CKJ"
FT HELIX 104..116
FT /evidence="ECO:0007829|PDB:7CKJ"
FT STRAND 121..127
FT /evidence="ECO:0007829|PDB:7CKJ"
FT HELIX 128..132
FT /evidence="ECO:0007829|PDB:7CKJ"
FT STRAND 137..143
FT /evidence="ECO:0007829|PDB:7CKJ"
FT HELIX 146..155
FT /evidence="ECO:0007829|PDB:7CKJ"
FT STRAND 157..159
FT /evidence="ECO:0007829|PDB:7CKJ"
FT HELIX 161..173
FT /evidence="ECO:0007829|PDB:7CKJ"
FT HELIX 176..178
FT /evidence="ECO:0007829|PDB:3AKC"
FT STRAND 186..189
FT /evidence="ECO:0007829|PDB:7CKJ"
FT TURN 190..192
FT /evidence="ECO:0007829|PDB:7CKJ"
FT HELIX 195..207
FT /evidence="ECO:0007829|PDB:7CKJ"
SQ SEQUENCE 208 AA; 22554 MW; 9D3B0F6BB95E0B84 CRC64;
MRGIVTIDGP SASGKSSVAR RVAAALGVPY LSSGLLYRAA AFLALRAGVD PGDEEGLLAL
LEGLGVRLLA QAEGNRVLAD GEDLTSFLHT PEVDRVVSAV ARLPGVRAWV NRRLKEVPPP
FVAEGRDMGT AVFPEAAHKF YLTASPEVRA WRRARERPQA YEEVLRDLLR RDERDKAQSA
PAPDALVLDT GGMTLDEVVA WVLAHIRR
//
