ID KEFG_ECOLI Reviewed; 184 AA.
AC P0A756; P45534; Q2M717;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2005, sequence version 1.
DT 24-JAN-2024, entry version 116.
DE RecName: Full=Glutathione-regulated potassium-efflux system ancillary protein KefG {ECO:0000255|HAMAP-Rule:MF_01415};
DE AltName: Full=Putative quinone oxidoreductase KefG {ECO:0000255|HAMAP-Rule:MF_01415};
DE EC=1.6.5.2 {ECO:0000255|HAMAP-Rule:MF_01415};
GN Name=kefG {ECO:0000255|HAMAP-Rule:MF_01415}; Synonyms=yheR;
GN OrderedLocusNames=b3351, JW3314;
OS Escherichia coli (strain K12).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP FUNCTION.
RC STRAIN=Frag5;
RX PubMed=11053405; DOI=10.1128/jb.182.22.6536-6540.2000;
RA Miller S., Ness L.S., Wood C.M., Fox B.C., Booth I.R.;
RT "Identification of an ancillary protein, YabF, required for activity of the
RT KefC glutathione-gated potassium efflux system in Escherichia coli.";
RL J. Bacteriol. 182:6536-6540(2000).
CC -!- FUNCTION: Regulatory subunit of a potassium efflux system that confers
CC protection against electrophiles. Required for full activity of KefB.
CC {ECO:0000255|HAMAP-Rule:MF_01415, ECO:0000269|PubMed:11053405}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a quinone + H(+) + NADH = a quinol + NAD(+);
CC Xref=Rhea:RHEA:46160, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124; EC=1.6.5.2;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01415};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a quinone + H(+) + NADPH = a quinol + NADP(+);
CC Xref=Rhea:RHEA:46164, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646,
CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:132124; EC=1.6.5.2;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01415};
CC -!- SUBUNIT: Interacts with KefB. {ECO:0000255|HAMAP-Rule:MF_01415}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01415}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01415}; Cytoplasmic side {ECO:0000255|HAMAP-Rule:MF_01415}.
CC -!- SIMILARITY: Belongs to the NAD(P)H dehydrogenase (quinone) family. KefG
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01415}.
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DR EMBL; U18997; AAA58148.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76376.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77939.1; -; Genomic_DNA.
DR PIR; B65129; B65129.
DR RefSeq; NP_417810.1; NC_000913.3.
DR AlphaFoldDB; P0A756; -.
DR SMR; P0A756; -.
DR BioGRID; 4263025; 13.
DR BioGRID; 852168; 1.
DR ComplexPortal; CPX-3090; Glutathione-regulated potassium-efflux system KefB-KefG complex.
DR IntAct; P0A756; 6.
DR STRING; 511145.b3351; -.
DR TCDB; 2.A.37.1.2; the monovalent cation:proton antiporter-2 (cpa2) family.
DR jPOST; P0A756; -.
DR PaxDb; 511145-b3351; -.
DR EnsemblBacteria; AAC76376; AAC76376; b3351.
DR GeneID; 947857; -.
DR KEGG; ecj:JW3314; -.
DR KEGG; eco:b3351; -.
DR PATRIC; fig|511145.12.peg.3445; -.
DR EchoBASE; EB2739; -.
DR eggNOG; COG2249; Bacteria.
DR HOGENOM; CLU_058643_0_1_6; -.
DR InParanoid; P0A756; -.
DR OMA; RYPMSDI; -.
DR PhylomeDB; P0A756; -.
DR BioCyc; EcoCyc:G7717-MONOMER; -.
DR PRO; PR:P0A756; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:1903103; C:potassium:proton antiporter complex; NAS:ComplexPortal.
DR GO; GO:0009055; F:electron transfer activity; IBA:GO_Central.
DR GO; GO:0010181; F:FMN binding; IBA:GO_Central.
DR GO; GO:0003955; F:NAD(P)H dehydrogenase (quinone) activity; IBA:GO_Central.
DR GO; GO:0050136; F:NADH dehydrogenase (quinone) activity; IEA:RHEA.
DR GO; GO:0008753; F:NADPH dehydrogenase (quinone) activity; IEA:RHEA.
DR GO; GO:1901381; P:positive regulation of potassium ion transmembrane transport; IMP:EcoCyc.
DR GO; GO:0006813; P:potassium ion transport; IEA:InterPro.
DR GO; GO:0051453; P:regulation of intracellular pH; NAS:ComplexPortal.
DR Gene3D; 3.40.50.360; -; 1.
DR HAMAP; MF_01415; K_H_efflux_KefG; 1.
DR InterPro; IPR003680; Flavodoxin_fold.
DR InterPro; IPR029039; Flavoprotein-like_sf.
DR InterPro; IPR023947; K_H_efflux_KefG.
DR InterPro; IPR046980; KefG/KefF.
DR PANTHER; PTHR47307; GLUTATHIONE-REGULATED POTASSIUM-EFFLUX SYSTEM ANCILLARY PROTEIN KEFG; 1.
DR PANTHER; PTHR47307:SF1; GLUTATHIONE-REGULATED POTASSIUM-EFFLUX SYSTEM ANCILLARY PROTEIN KEFG; 1.
DR Pfam; PF02525; Flavodoxin_2; 1.
DR SUPFAM; SSF52218; Flavoproteins; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; NAD; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..184
FT /note="Glutathione-regulated potassium-efflux system
FT ancillary protein KefG"
FT /id="PRO_0000071642"
SQ SEQUENCE 184 AA; 20958 MW; F3B1123F7378EAB7 CRC64;
MMSQPAKVLL LYAHPESQDS VANRVLLKPA TQLSNVTVHD LYAHYPDFFI DIPREQALLR
EHEVIVFQHP LYTYSCPALL KEWLDRVLSR GFASGPGGNQ LAGKYWRSVI TTGEPESAYR
YDALNRYPMS DVLRPFELAA GMCRMHWLSP IIIYWARRQS AQELASHARA YGDWLANPLS
PGGR
//
