ID KHK_PONAB Reviewed; 297 AA.
AC Q5RD71;
DT 24-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 27-MAR-2024, entry version 73.
DE RecName: Full=Ketohexokinase;
DE EC=2.7.1.3;
DE AltName: Full=Hepatic fructokinase;
GN Name=KHK;
OS Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Pongo.
OX NCBI_TaxID=9601;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RG The German cDNA consortium;
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the phosphorylation of the ketose sugar fructose to
CC fructose-1-phosphate. {ECO:0000250|UniProtKB:P50053}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + beta-D-fructose = ADP + beta-D-fructose 1-phosphate +
CC H(+); Xref=Rhea:RHEA:18145, ChEBI:CHEBI:15378, ChEBI:CHEBI:28645,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:138881, ChEBI:CHEBI:456216;
CC EC=2.7.1.3;
CC -!- ACTIVITY REGULATION: Requires potassium. Inhibition by ADP (By
CC similarity). {ECO:0000250}.
CC -!- PATHWAY: Carbohydrate metabolism; fructose metabolism.
CC -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:P50053}.
CC -!- SIMILARITY: Belongs to the carbohydrate kinase PfkB family.
CC {ECO:0000305}.
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DR EMBL; CR858045; CAH90286.1; -; Transcribed_RNA.
DR AlphaFoldDB; Q5RD71; -.
DR SMR; Q5RD71; -.
DR STRING; 9601.ENSPPYP00000014032; -.
DR eggNOG; KOG2947; Eukaryota.
DR InParanoid; Q5RD71; -.
DR UniPathway; UPA00202; -.
DR Proteomes; UP000001595; Unplaced.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004454; F:ketohexokinase activity; IEA:UniProtKB-EC.
DR GO; GO:0006000; P:fructose metabolic process; IEA:UniProtKB-UniPathway.
DR CDD; cd01939; Ketohexokinase; 1.
DR Gene3D; 3.40.1190.20; -; 1.
DR InterPro; IPR034093; KHK.
DR InterPro; IPR011611; PfkB_dom.
DR InterPro; IPR029056; Ribokinase-like.
DR PANTHER; PTHR42774:SF3; KETOHEXOKINASE ISOFORM X1; 1.
DR PANTHER; PTHR42774; PHOSPHOTRANSFERASE SYSTEM TRANSPORT PROTEIN; 1.
DR Pfam; PF00294; PfkB; 1.
DR SUPFAM; SSF53613; Ribokinase-like; 1.
PE 3: Inferred from homology;
KW ATP-binding; Carbohydrate metabolism; Kinase; Nucleotide-binding;
KW Reference proteome; Transferase.
FT CHAIN 1..297
FT /note="Ketohexokinase"
FT /id="PRO_0000080090"
FT BINDING 15
FT /ligand="beta-D-fructose"
FT /ligand_id="ChEBI:CHEBI:28645"
FT /evidence="ECO:0000250|UniProtKB:P50053"
FT BINDING 41
FT /ligand="beta-D-fructose"
FT /ligand_id="ChEBI:CHEBI:28645"
FT /evidence="ECO:0000250|UniProtKB:P50053"
FT BINDING 42
FT /ligand="beta-D-fructose"
FT /ligand_id="ChEBI:CHEBI:28645"
FT /evidence="ECO:0000250|UniProtKB:P50053"
FT BINDING 45
FT /ligand="beta-D-fructose"
FT /ligand_id="ChEBI:CHEBI:28645"
FT /evidence="ECO:0000250|UniProtKB:P50053"
FT BINDING 107
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:P50053"
FT BINDING 225..228
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:P50053"
FT BINDING 254..257
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:P50053"
FT BINDING 257
FT /ligand="beta-D-fructose"
FT /ligand_id="ChEBI:CHEBI:28645"
FT /evidence="ECO:0000250|UniProtKB:P50053"
SQ SEQUENCE 297 AA; 32476 MW; 049C7C1FA74DEF16 CRC64;
MEEKQILCVG LVVLDVISLV DKYPKEDSEI RCLSQRWQRG GNASNSCTVL SLLGAPCAFM
GSMAPGHVAF LVADFRRRGV DVSQVAWQSK GDTPSSCCII NNSNGNRTIV LHDTSLPDVS
ATDFEKVDLT QFKWIHIEGR NASEQVKMLQ RIDTHNTRQP PEQKIRVSVE VEKPQEELFQ
LFGYGDVVFV SKDVAKHLGF QSAEAALRGL YGRVRKGAVL VCAWAEEGAD ALGPDDKLFH
SDAFPPPRVV DTLGAGDTFN ASVIFSLSQG RSMQEALRFG CQVAGKKCGL QGFDGIV
//
