ID   L0M3Z6_ENTBF            Unreviewed;       521 AA.
AC   L0M3Z6;
DT   06-MAR-2013, integrated into UniProtKB/TrEMBL.
DT   06-MAR-2013, sequence version 1.
DT   27-MAR-2024, entry version 43.
DE   RecName: Full=cyclic-guanylate-specific phosphodiesterase {ECO:0000256|ARBA:ARBA00012282};
DE            EC=3.1.4.52 {ECO:0000256|ARBA:ARBA00012282};
GN   OrderedLocusNames=D782_1449 {ECO:0000313|EMBL:AGB77460.1};
OS   Enterobacteriaceae bacterium (strain FGI 57).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae.
OX   NCBI_TaxID=693444 {ECO:0000313|EMBL:AGB77460.1, ECO:0000313|Proteomes:UP000011002};
RN   [1] {ECO:0000313|EMBL:AGB77460.1, ECO:0000313|Proteomes:UP000011002}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=FGI 57 {ECO:0000313|EMBL:AGB77460.1,
RC   ECO:0000313|Proteomes:UP000011002};
RX   PubMed=23469353; DOI=10.1128/genomeA.00238-12;
RA   Aylward F.O., Tremmel D.M., Bruce D.C., Chain P., Chen A.,
RA   Walston Davenport K., Detter C., Han C.S., Han J., Huntemann M.,
RA   Ivanova N.N., Kyrpides N.C., Markowitz V., Mavrommatis K., Nolan M.,
RA   Pagani I., Pati A., Pitluck S., Deshpande S., Goodwin L., Woyke T.,
RA   Currie C.R.;
RT   "Complete genome of Enterobacteriaceae bacterium strain FGI 57, a strain
RT   associated with leaf-cutter ant fungus gardens.";
RL   Genome Announc. 1:E00238-12(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3',3'-c-di-GMP + H2O = 5'-phosphoguanylyl(3'->5')guanosine +
CC         H(+); Xref=Rhea:RHEA:24902, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:58754, ChEBI:CHEBI:58805; EC=3.1.4.52;
CC         Evidence={ECO:0000256|ARBA:ARBA00034290};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC       pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
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DR   EMBL; CP003938; AGB77460.1; -; Genomic_DNA.
DR   AlphaFoldDB; L0M3Z6; -.
DR   STRING; 693444.D782_1449; -.
DR   KEGG; ebf:D782_1449; -.
DR   PATRIC; fig|693444.3.peg.1391; -.
DR   eggNOG; COG2200; Bacteria.
DR   HOGENOM; CLU_000445_131_2_6; -.
DR   OrthoDB; 675397at2; -.
DR   Proteomes; UP000011002; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR   GO; GO:0071111; F:cyclic-guanylate-specific phosphodiesterase activity; IEA:UniProtKB-EC.
DR   CDD; cd01948; EAL; 1.
DR   Gene3D; 3.20.20.450; EAL domain; 1.
DR   InterPro; IPR024744; CSS-motif_dom.
DR   InterPro; IPR001633; EAL_dom.
DR   InterPro; IPR035919; EAL_sf.
DR   PANTHER; PTHR33121; CYCLIC DI-GMP PHOSPHODIESTERASE PDEF; 1.
DR   PANTHER; PTHR33121:SF78; CYCLIC DI-GMP PHOSPHODIESTERASE PDEI-RELATED; 1.
DR   Pfam; PF12792; CSS-motif; 1.
DR   Pfam; PF00563; EAL; 1.
DR   SMART; SM00052; EAL; 1.
DR   SUPFAM; SSF141868; EAL domain-like; 1.
DR   PROSITE; PS50883; EAL; 1.
PE   4: Predicted;
KW   c-di-GMP {ECO:0000256|ARBA:ARBA00022636};
KW   Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW   Reference proteome {ECO:0000313|Proteomes:UP000011002};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        12..34
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        237..259
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          262..515
FT                   /note="EAL"
FT                   /evidence="ECO:0000259|PROSITE:PS50883"
SQ   SEQUENCE   521 AA;  57859 MW;  CBFBF6613DFFF5D7 CRC64;
     MLSRKTLLSR RIAITSLLVG VFFALLAGGT QFLVSYHKRT EQFDNTITEL KTYLNSYFGE
     VLNTIDTLQP LTMSSCDNVS SELTSRAAFS LNIRAFLLVK EGHAFCSSAT GPLDIAIKDL
     VPSLDLSQNQ AMAILPGTPM MPHRPAFILW DRSPLLADRG VFTSINANLM PWILNSSKQS
     QYSGIALVIN GDTAISTFNH QPVALASIEN TPIRRAKING VPLEIYLYGN EVATDNMLYS
     LLLGAMCGLL SGILCAYVMT IRMRPGREIL SAITRNQFYV VYQPVVHASD LHIGGVEVLM
     RWTHPVAGNI PPDAFISFAE GQNLIVPLTR HLFDLIAKDA PALQKVLPPG AKLGINISPS
     HLHAESFKQD IRHLLASLPE NYFQVVLELT ERDMLNEKQA VKLFEWLHSE GIEIAIDDFG
     TGHSALIYLE RFTLDYLKID RGFVNAIGME TVTSPVLDTV LTLTHRLNLV TVAEGVETQE
     QAAWLRDHGV DFLQGYWISR PLTLTQLVEA HNEPAKYFTS R
//