ID L5K6Z3_PTEAL Unreviewed; 751 AA.
AC L5K6Z3;
DT 06-MAR-2013, integrated into UniProtKB/TrEMBL.
DT 06-MAR-2013, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=tRNA (uracil-O(2)-)-methyltransferase {ECO:0000256|RuleBase:RU368004};
DE EC=2.1.1.211 {ECO:0000256|RuleBase:RU368004};
GN ORFNames=PAL_GLEAN10022849 {ECO:0000313|EMBL:ELK06546.1};
OS Pteropus alecto (Black flying fox).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Chiroptera; Megachiroptera; Pteropodidae;
OC Pteropodinae; Pteropus.
OX NCBI_TaxID=9402 {ECO:0000313|EMBL:ELK06546.1, ECO:0000313|Proteomes:UP000010552};
RN [1] {ECO:0000313|Proteomes:UP000010552}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=23258410; DOI=10.1126/science.1230835;
RA Zhang G., Cowled C., Shi Z., Huang Z., Bishop-Lilly K.A., Fang X.,
RA Wynne J.W., Xiong Z., Baker M.L., Zhao W., Tachedjian M., Zhu Y., Zhou P.,
RA Jiang X., Ng J., Yang L., Wu L., Xiao J., Feng Y., Chen Y., Sun X.,
RA Zhang Y., Marsh G.A., Crameri G., Broder C.C., Frey K.G., Wang L.F.,
RA Wang J.;
RT "Comparative analysis of bat genomes provides insight into the evolution of
RT flight and immunity.";
RL Science 339:456-460(2013).
CC -!- FUNCTION: Adenosyl-L-methionine (AdoMet)-dependent tRNA (uracil-O(2)-)-
CC methyltransferase. {ECO:0000256|RuleBase:RU368004}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-adenosyl-L-methionine + uridine(44) in tRNA(Ser) = 2'-O-
CC methyluridine(44) in tRNA(Ser) + H(+) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:43100, Rhea:RHEA-COMP:10339, Rhea:RHEA-COMP:10340,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:65315, ChEBI:CHEBI:74478; EC=2.1.1.211;
CC Evidence={ECO:0000256|ARBA:ARBA00000660,
CC ECO:0000256|RuleBase:RU368004};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|RuleBase:RU368004}.
CC -!- SIMILARITY: Belongs to the TRM44 family.
CC {ECO:0000256|ARBA:ARBA00009056, ECO:0000256|RuleBase:RU368004}.
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DR EMBL; KB031030; ELK06546.1; -; Genomic_DNA.
DR RefSeq; XP_006919079.1; XM_006919017.2.
DR AlphaFoldDB; L5K6Z3; -.
DR STRING; 9402.L5K6Z3; -.
DR GeneID; 102892619; -.
DR KEGG; pale:102892619; -.
DR CTD; 152992; -.
DR eggNOG; KOG3790; Eukaryota.
DR InParanoid; L5K6Z3; -.
DR OrthoDB; 1353816at2759; -.
DR Proteomes; UP000010552; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0141101; F:tRNA(Ser) (uridine(44)-2'-O-)-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0030488; P:tRNA methylation; IEA:UniProtKB-UniRule.
DR InterPro; IPR011671; tRNA_uracil_MeTrfase.
DR PANTHER; PTHR21210:SF0; TRNA (URACIL-O(2)-)-METHYLTRANSFERASE-RELATED; 1.
DR PANTHER; PTHR21210; UNCHARACTERIZED; 1.
DR Pfam; PF07757; AdoMet_MTase; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|RuleBase:RU368004};
KW Methyltransferase {ECO:0000256|RuleBase:RU368004,
KW ECO:0000313|EMBL:ELK06546.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000010552};
KW S-adenosyl-L-methionine {ECO:0000256|RuleBase:RU368004};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU368004};
KW tRNA processing {ECO:0000256|RuleBase:RU368004}.
FT REGION 45..137
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 526..592
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 62..82
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 101..116
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 751 AA; 83327 MW; 67DC0A685698417E CRC64;
MAEAGRVAVR DPGALLPDGF WAAVAVWLER PQVANKRLCG ARLEARRSAA LPRTGARGPR
QSTGPEREER ATEGHGPEEG RLLGQRPPEA GPGPRSSAGL EESQGRRHKG EDEREAAAAP
LLAGAPGRPG EAGVGEGDLP AWDLDSLWDD FSRSLGGGSQ DMLAFLTGSG AGSQSEAQHQ
LDVVLRTVIP KRNPLSALRR EIVVQDVHNG TVTFLPLEED DKGNVSVQLS NVYQIQLRHS
EEQWFISVLI FCPERWHSDG IVYPKLTWLG EELLAKLARW AVESRKSEFK RTLSLVSIVK
YSQVYQELKD KYKDMVKVWP EVTDPEKFVY EDVAIASYLL VLWEEERAER GVTAKQSFVD
LGCGNGLLVH ILSSEGHPGR GIDVRRRKIW DMYGPQTRLE EVAITPNDRT LFPDVDWLIG
NHSDELTPWI PVIAARSSYS CRFFVLPCCF FDFVGKHCRR QSGKTQYRGY LDFISEVGRA
CGFRVEEDCL RIPSTRRVCL LGKSRTYPPA GEASVDAQRT RYINSRQGRA ASRPSCPDEG
PAPPPRGSTD QPGSHPGGCP DSRPDSQGAP DAGADGRWLS GFRPREKAER GRNCAALPRE
FVDGVVVQVA QRLLGGKRLD TGGSRSGTVG TWNRGESLSL AEVAGELGGE TLRRLKRECG
GLQTLLRNNR QVFEVLNGRV RIRDWRQEKP LQEKQLEAKR RASSEAFKTR LCWFFVHHPD
GCVLPAHCCP FAHGPGELRP PPPTRKKQQH L
//
