ID L7LHM6_9ACTN Unreviewed; 950 AA.
AC L7LHM6;
DT 06-MAR-2013, integrated into UniProtKB/TrEMBL.
DT 06-MAR-2013, sequence version 1.
DT 24-JAN-2024, entry version 46.
DE RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00022419, ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPC {ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_00595};
DE EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305, ECO:0000256|HAMAP-Rule:MF_00595};
GN Name=ppc {ECO:0000256|HAMAP-Rule:MF_00595,
GN ECO:0000313|EMBL:GAC59592.1};
GN ORFNames=GSI01S_03_00510 {ECO:0000313|EMBL:GAC59592.1};
OS Gordonia sihwensis NBRC 108236.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Gordoniaceae;
OC Gordonia.
OX NCBI_TaxID=1223544 {ECO:0000313|EMBL:GAC59592.1, ECO:0000313|Proteomes:UP000035083};
RN [1] {ECO:0000313|EMBL:GAC59592.1, ECO:0000313|Proteomes:UP000035083}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 108236 {ECO:0000313|EMBL:GAC59592.1,
RC ECO:0000313|Proteomes:UP000035083};
RA Yoshida I., Hosoyama A., Tsuchikane K., Ando Y., Baba S., Ohji S.,
RA Hamada M., Tamura T., Yamazoe A., Yamazaki S., Fujita N.;
RT "Whole genome shotgun sequence of Gordonia sihwensis NBRC 108236.";
RL Submitted (DEC-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC for the tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003670,
CC ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC Evidence={ECO:0000256|ARBA:ARBA00001071, ECO:0000256|HAMAP-
CC Rule:MF_00595};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946,
CC ECO:0000256|HAMAP-Rule:MF_00595};
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC {ECO:0000256|ARBA:ARBA00008346, ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAC59592.1}.
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DR EMBL; BANU01000003; GAC59592.1; -; Genomic_DNA.
DR RefSeq; WP_006894842.1; NZ_BANU01000003.1.
DR AlphaFoldDB; L7LHM6; -.
DR eggNOG; COG2352; Bacteria.
DR Proteomes; UP000035083; Unassembled WGS sequence.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR HAMAP; MF_00595; PEPcase_type1; 1.
DR InterPro; IPR021135; PEP_COase.
DR InterPro; IPR022805; PEP_COase_bac/pln-type.
DR InterPro; IPR018129; PEP_COase_Lys_AS.
DR InterPro; IPR033129; PEPCASE_His_AS.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR Pfam; PF00311; PEPcase; 1.
DR PRINTS; PR00150; PEPCARBXLASE.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR PROSITE; PS00781; PEPCASE_1; 1.
DR PROSITE; PS00393; PEPCASE_2; 1.
PE 3: Inferred from homology;
KW Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300, ECO:0000256|HAMAP-
KW Rule:MF_00595};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00595};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00595};
KW Pyruvate {ECO:0000313|EMBL:GAC59592.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000035083}.
FT ACT_SITE 169
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT ECO:0000256|PROSITE-ProRule:PRU10111"
FT ACT_SITE 613
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT ECO:0000256|PROSITE-ProRule:PRU10112"
SQ SEQUENCE 950 AA; 103516 MW; 5DF77904B79470A9 CRC64;
MASHSLTPET LARAIATPMV DRISVDDTGR ALTEPLREDI RFLGGLLGEV IRSHAGEATF
ELVESSRRDA FGIRYAEVTR DQLAGRYDGR AVADLMPVIR AFTNFALLAN LAEDLHRERR
RRIHQRAGDP APPSSLAATF GKLADAGRTD ADVAAALRSA EVVPVITAHP TETRRRSVFD
AQNRITELMR VRARTELTPE EDARLTEDIR RQILVLWQTA LIRLRRLTIS DEITTGLRYY
GASFFDVVPA LNKDVRAALV EAYPDAGLDG LSMISMGSWI GGDRDGNPFV SAEVVDEAAT
SAARTAMRHH LDELAALHQE LSLSARLFSP VSPALSALGS GFAPPDEPGD TDDEPFRSAV
SAVRSRVLAR ALRTLGEDAV DPAELDTARA AAPYGDADEF LADLDVIDRA LRAAHDDSIA
DDRLATLREA VRTFGFHLSG LDMRQNSETH EQVVAELLSW AGVCADYPAL PEADRIGLLT
AELASRRPLT TPDAELSELA MKELGVVHAA AGAIERFGPR AVPNYVISMC SSVSDLLEVL
ILLKEAGIYH AAGPGDAGPS CSVRVVPLFE TIEDLRGGAA TLTAALNLGF YRALVRAQGD
VQEVMLGYSD SNKDGGYLAA NWALYRAELD LVSASRAAEV RLRLFHGRGG TVGRGGGPSY
DAILAQPPGA VTGSLRLTEQ GEIIAAKYAE PVSARRNLES LLAATIESSL LDVEGLDDPE
RAYADFDELA ESARRAYSAL VHETPGFVEY FTASTPLSEI GALNIGSRPT SRKQTTAISD
LRAIPWVLSW TQCRVMLPGW YGVGSAFAQW TGGDDARIAD LRRYYENWPF FRSVMSNMAQ
VLAKSDMGLA SRYAELVPDE ELRRRVFSMI VEEHERTLEM YTAITGTTDL LADNDALKRS
VYNRFPYLEP LNLLQVELLQ RFRSGEDSPL IRRGIQLTMN GLATALRNSG
//
