ID L7ZVZ0_9BACI Unreviewed; 515 AA.
AC L7ZVZ0;
DT 03-APR-2013, integrated into UniProtKB/TrEMBL.
DT 03-APR-2013, sequence version 1.
DT 27-MAR-2024, entry version 43.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=GHH_c13080 {ECO:0000313|EMBL:AGE21845.1};
OS Geobacillus sp. GHH01.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Geobacillus.
OX NCBI_TaxID=1233873 {ECO:0000313|EMBL:AGE21845.1, ECO:0000313|Proteomes:UP000011251};
RN [1] {ECO:0000313|EMBL:AGE21845.1, ECO:0000313|Proteomes:UP000011251}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GHH01 {ECO:0000313|EMBL:AGE21845.1};
RX PubMed=23618712;
RA Wiegand S., Rabausch U., Chow J., Daniel R., Streit W.R., Liesegang H.;
RT "Complete Genome Sequence of Geobacillus sp. Strain GHH01, a Thermophilic
RT Lipase-Secreting Bacterium.";
RL Genome Announc. 1:E0009213-E0009213(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR EMBL; CP004008; AGE21845.1; -; Genomic_DNA.
DR RefSeq; WP_015374511.1; NC_020210.1.
DR AlphaFoldDB; L7ZVZ0; -.
DR REBASE; 60338; M.GspGHH01ORF13080P.
DR KEGG; ggh:GHH_c13080; -.
DR PATRIC; fig|1233873.3.peg.1383; -.
DR HOGENOM; CLU_013049_4_1_9; -.
DR Proteomes; UP000011251; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR PANTHER; PTHR42998:SF1; TYPE I RESTRICTION ENZYME HINDI METHYLASE SUBUNIT; 1.
DR PANTHER; PTHR42998; TYPE I RESTRICTION ENZYME HINDVIIP M PROTEIN-RELATED; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000313|EMBL:AGE21845.1};
KW Transferase {ECO:0000313|EMBL:AGE21845.1}.
FT DOMAIN 10..150
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 164..477
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 515 AA; 58684 MW; 61BA735F08B29EE1 CRC64;
MTVKADIDFQ KDLFEAANKM RGSVAPADYK HYVLPLIFLR YLSNKYEQRR KELEQIVKDP
SSDWYTEDDE MRQVIITDPD QYKAENVFVV PEEASWSYIM KNAKQPNIKE ILDNAMKRLE
EENPELEGIL PRIYQGSNLP PENVAGLIEI FSRDVFSANT DDSVDILGRT YEYFISSFAA
SEGNRGGEFF TPSSIVKLLV AMLEPKSGIV FDPACGSGGM FIQSEEYAPN KHALSFYGQE
NVVTTVRLGK MNVLLHGINA EIRLGDSLLN DQFPDLKADY VIANPPFNQK DWGADRLSKN
DPRLIGPVTN SNANYMWMQH FLYHLNDTGT AGFVMANGAM TTNVKEEKEV RQKLVDEGYI
DCIVQLPEKL FFTTGIPCCL FFLSKNRDGK NGYRARKNEI LFIDARKMGT LVSRKQKALS
KEEIDKIAAV YHAYKYEEAE QYEDVAGFCK VATIDEVRAN DYKLTPGIYV GTEVSNEDDV
PFEEKMAELT QRLLEQFEES NRLQEKIKKD LEGLM
//
