ID LACG_STAAT Reviewed; 470 AA.
AC A8YYF6;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 1.
DT 27-MAR-2024, entry version 84.
DE RecName: Full=6-phospho-beta-galactosidase {ECO:0000255|HAMAP-Rule:MF_01574};
DE EC=3.2.1.85 {ECO:0000255|HAMAP-Rule:MF_01574};
DE AltName: Full=Beta-D-phosphogalactoside galactohydrolase {ECO:0000255|HAMAP-Rule:MF_01574};
DE Short=PGALase {ECO:0000255|HAMAP-Rule:MF_01574};
DE AltName: Full=P-beta-Gal {ECO:0000255|HAMAP-Rule:MF_01574};
DE Short=PBG {ECO:0000255|HAMAP-Rule:MF_01574};
GN Name=lacG {ECO:0000255|HAMAP-Rule:MF_01574};
GN OrderedLocusNames=USA300HOU_2182;
OS Staphylococcus aureus (strain USA300 / TCH1516).
OC Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=451516;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=USA300 / TCH1516;
RX PubMed=17986343; DOI=10.1186/1471-2180-7-99;
RA Highlander S.K., Hulten K.G., Qin X., Jiang H., Yerrapragada S.,
RA Mason E.O. Jr., Shang Y., Williams T.M., Fortunov R.M., Liu Y., Igboeli O.,
RA Petrosino J., Tirumalai M., Uzman A., Fox G.E., Cardenas A.M., Muzny D.M.,
RA Hemphill L., Ding Y., Dugan S., Blyth P.R., Buhay C.J., Dinh H.H.,
RA Hawes A.C., Holder M., Kovar C.L., Lee S.L., Liu W., Nazareth L.V.,
RA Wang Q., Zhou J., Kaplan S.L., Weinstock G.M.;
RT "Subtle genetic changes enhance virulence of methicillin resistant and
RT sensitive Staphylococcus aureus.";
RL BMC Microbiol. 7:99-99(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 6-phospho-beta-D-galactoside + H2O = an alcohol + D-
CC galactose 6-phosphate; Xref=Rhea:RHEA:24568, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:30879, ChEBI:CHEBI:58534, ChEBI:CHEBI:91004; EC=3.2.1.85;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01574};
CC -!- PATHWAY: Carbohydrate metabolism; lactose degradation; D-galactose 6-
CC phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1.
CC {ECO:0000255|HAMAP-Rule:MF_01574}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family.
CC {ECO:0000255|HAMAP-Rule:MF_01574}.
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DR EMBL; CP000730; ABX30176.1; -; Genomic_DNA.
DR RefSeq; WP_000169220.1; NC_010079.1.
DR AlphaFoldDB; A8YYF6; -.
DR SMR; A8YYF6; -.
DR CAZy; GH1; Glycoside Hydrolase Family 1.
DR KEGG; sax:USA300HOU_2182; -.
DR HOGENOM; CLU_001859_1_3_9; -.
DR UniPathway; UPA00542; UER00605.
DR GO; GO:0033920; F:6-phospho-beta-galactosidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019512; P:lactose catabolic process via tagatose-6-phosphate; IEA:InterPro.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR HAMAP; MF_01574; LacG; 1.
DR InterPro; IPR005928; 6P-beta-galactosidase.
DR InterPro; IPR001360; Glyco_hydro_1.
DR InterPro; IPR018120; Glyco_hydro_1_AS.
DR InterPro; IPR033132; Glyco_hydro_1_N_CS.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR NCBIfam; TIGR01233; lacG; 1.
DR PANTHER; PTHR10353:SF340; 6-PHOSPHO-BETA-GALACTOSIDASE 1; 1.
DR PANTHER; PTHR10353; GLYCOSYL HYDROLASE; 1.
DR Pfam; PF00232; Glyco_hydro_1; 1.
DR PRINTS; PR00131; GLHYDRLASE1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS00572; GLYCOSYL_HYDROL_F1_1; 1.
DR PROSITE; PS00653; GLYCOSYL_HYDROL_F1_2; 1.
PE 3: Inferred from homology;
KW Glycosidase; Hydrolase.
FT CHAIN 1..470
FT /note="6-phospho-beta-galactosidase"
FT /id="PRO_1000087881"
FT ACT_SITE 160
FT /note="Proton donor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01574"
FT ACT_SITE 375
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01574"
FT BINDING 19
FT /ligand="D-galactose 6-phosphate"
FT /ligand_id="ChEBI:CHEBI:91004"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01574"
FT BINDING 116
FT /ligand="D-galactose 6-phosphate"
FT /ligand_id="ChEBI:CHEBI:91004"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01574"
FT BINDING 159
FT /ligand="D-galactose 6-phosphate"
FT /ligand_id="ChEBI:CHEBI:91004"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01574"
FT BINDING 160
FT /ligand="D-galactose 6-phosphate"
FT /ligand_id="ChEBI:CHEBI:91004"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01574"
FT BINDING 297
FT /ligand="D-galactose 6-phosphate"
FT /ligand_id="ChEBI:CHEBI:91004"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01574"
FT BINDING 430
FT /ligand="D-galactose 6-phosphate"
FT /ligand_id="ChEBI:CHEBI:91004"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01574"
FT BINDING 431
FT /ligand="D-galactose 6-phosphate"
FT /ligand_id="ChEBI:CHEBI:91004"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01574"
FT BINDING 437
FT /ligand="D-galactose 6-phosphate"
FT /ligand_id="ChEBI:CHEBI:91004"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01574"
FT BINDING 439
FT /ligand="D-galactose 6-phosphate"
FT /ligand_id="ChEBI:CHEBI:91004"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01574"
SQ SEQUENCE 470 AA; 54550 MW; 9A50A44B5ABFE74F CRC64;
MTKTLPEDFI FGGATAAYQA EGATNTDGKG RVAWDTYLEE NYWYTAEPAS DFYNRYPVDL
ELSEKFGVNG IRISIAWSRI FPNGYGEVNP KGVEYYHKLF AECHKRHVEP FVTLHHFDTP
EVLHKDGDFL NRKTIDYFVD YAEYCFKEFP EVKYWTTFNE IGPIGDGQYL VGKFPPGIKY
DFEKVFQSHH NMMVAHARAV KLFKDGGYKG EIGVVHALPT KYPFDPSNPE DVRAAELEDI
IHNKFILDAT YLGKYSRETM EGVQHILSVN GGKLNITDED YAILDAAKDL NDFLGINYYM
SDWMRGYDGE SEITHNATGD KGGSKYQLKG VGQREFDVDV PRTDWDWMIY PQGLYDQIMR
VVKDYPNYHK IYITENGLGY KDEFIESEKT VHDDARIDYV RQHLNVIADA IIDGANVKGY
FIWSLMDVFS WSNGYEKRYG LFYVDFETQE RYPKKSAYWY KELAETKEIK
//
