ID LSM3_MOUSE Reviewed; 102 AA.
AC P62311; Q9Y4Z1;
DT 05-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 24-JAN-2024, entry version 136.
DE RecName: Full=U6 snRNA-associated Sm-like protein LSm3;
GN Name=Lsm3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Heart, and Pancreas;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and FVB/N; TISSUE=Brain, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Heart, and Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Plays a role in pre-mRNA splicing as component of the U4/U6-
CC U5 tri-snRNP complex that is involved in spliceosome assembly, and as
CC component of the precatalytic spliceosome (spliceosome B complex). The
CC heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract
CC of U6 snRNA. {ECO:0000250|UniProtKB:P62310}.
CC -!- SUBUNIT: Component of the precatalytic spliceosome (spliceosome B
CC complex). Component of the U4/U6-U5 tri-snRNP complex, a building block
CC of the precatalytic spliceosome (spliceosome B complex). The U4/U6-U5
CC tri-snRNP complex is composed of the U4, U6 and U5 snRNAs and at least
CC PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB,
CC SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH,
CC SNU13, EFTUD2, SART1 and USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7
CC and LSM8. LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8 form a
CC heptameric, ring-shaped subcomplex (the LSM2-8 complex) that is part of
CC the U4/U6-U5 tri-snRNP complex and the precatalytic spliceosome.
CC {ECO:0000250|UniProtKB:P62310}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P62310}.
CC -!- SIMILARITY: Belongs to the snRNP Sm proteins family. {ECO:0000305}.
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DR EMBL; AK003125; BAB22585.1; -; mRNA.
DR EMBL; AK007849; BAB25303.1; -; mRNA.
DR EMBL; AK021251; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BC054368; AAH54368.1; -; mRNA.
DR EMBL; BC062875; AAH62875.1; -; mRNA.
DR CCDS; CCDS20369.1; -.
DR RefSeq; NP_080585.1; NM_026309.2.
DR AlphaFoldDB; P62311; -.
DR SMR; P62311; -.
DR BioGRID; 212360; 46.
DR IntAct; P62311; 1.
DR MINT; P62311; -.
DR STRING; 10090.ENSMUSP00000044178; -.
DR PhosphoSitePlus; P62311; -.
DR REPRODUCTION-2DPAGE; IPI00230388; -.
DR REPRODUCTION-2DPAGE; P62311; -.
DR EPD; P62311; -.
DR jPOST; P62311; -.
DR MaxQB; P62311; -.
DR PaxDb; 10090-ENSMUSP00000044178; -.
DR PeptideAtlas; P62311; -.
DR ProteomicsDB; 293405; -.
DR Pumba; P62311; -.
DR Antibodypedia; 26522; 125 antibodies from 21 providers.
DR DNASU; 67678; -.
DR Ensembl; ENSMUST00000040607.6; ENSMUSP00000044178.5; ENSMUSG00000034192.6.
DR Ensembl; ENSMUST00000206947.2; ENSMUSP00000145568.2; ENSMUSG00000034192.6.
DR GeneID; 67678; -.
DR KEGG; mmu:67678; -.
DR UCSC; uc009cye.1; mouse.
DR AGR; MGI:1914928; -.
DR CTD; 27258; -.
DR MGI; MGI:1914928; Lsm3.
DR VEuPathDB; HostDB:ENSMUSG00000034192; -.
DR eggNOG; KOG3460; Eukaryota.
DR GeneTree; ENSGT00390000013951; -.
DR HOGENOM; CLU_076902_5_1_1; -.
DR InParanoid; P62311; -.
DR OMA; QAFDSHC; -.
DR OrthoDB; 5485754at2759; -.
DR PhylomeDB; P62311; -.
DR TreeFam; TF312907; -.
DR Reactome; R-MMU-430039; mRNA decay by 5' to 3' exoribonuclease.
DR Reactome; R-MMU-72163; mRNA Splicing - Major Pathway.
DR BioGRID-ORCS; 67678; 29 hits in 82 CRISPR screens.
DR ChiTaRS; Lsm3; mouse.
DR PRO; PR:P62311; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; P62311; Protein.
DR Bgee; ENSMUSG00000034192; Expressed in epiblast (generic) and 74 other cell types or tissues.
DR Genevisible; P62311; MM.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; ISO:MGI.
DR GO; GO:1990726; C:Lsm1-7-Pat1 complex; IBA:GO_Central.
DR GO; GO:0120115; C:Lsm2-8 complex; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0000932; C:P-body; IBA:GO_Central.
DR GO; GO:0071011; C:precatalytic spliceosome; IBA:GO_Central.
DR GO; GO:0071005; C:U2-type precatalytic spliceosome; ISS:UniProtKB.
DR GO; GO:0046540; C:U4/U6 x U5 tri-snRNP complex; ISS:UniProtKB.
DR GO; GO:0005688; C:U6 snRNP; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0030629; F:U6 snRNA 3'-end binding; ISO:MGI.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:0033962; P:P-body assembly; IBA:GO_Central.
DR CDD; cd01730; LSm3; 1.
DR Gene3D; 2.30.30.100; -; 1.
DR InterPro; IPR034105; Lsm3.
DR InterPro; IPR010920; LSM_dom_sf.
DR InterPro; IPR047575; Sm.
DR InterPro; IPR040002; Sm-like_LSM3.
DR InterPro; IPR001163; Sm_dom_euk/arc.
DR PANTHER; PTHR13110; U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM3; 1.
DR PANTHER; PTHR13110:SF15; U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM3; 1.
DR Pfam; PF01423; LSM; 1.
DR SMART; SM00651; Sm; 1.
DR SUPFAM; SSF50182; Sm-like ribonucleoproteins; 1.
DR PROSITE; PS52002; SM; 1.
PE 1: Evidence at protein level;
KW Acetylation; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW Ribonucleoprotein; RNA-binding; Spliceosome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:P62310"
FT CHAIN 2..102
FT /note="U6 snRNA-associated Sm-like protein LSm3"
FT /id="PRO_0000125561"
FT DOMAIN 16..101
FT /note="Sm"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01346"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:P62310"
SQ SEQUENCE 102 AA; 11845 MW; 206B0DF0D95CD4D2 CRC64;
MADDVDQQQT TNTVEEPLDL IRLSLDERIY VKMRNDRELR GRLHAYDQHL NMILGDVEET
VTTIEIDEET YEEIYKSTKR NIPMLFVRGD GVVLVAPPLR VG
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