ID LSM4_DICDI Reviewed; 177 AA.
AC Q54KX4;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 24-JAN-2024, entry version 101.
DE RecName: Full=Probable U6 snRNA-associated Sm-like protein LSm4;
GN Name=lsm4; ORFNames=DDB_G0287053;
OS Dictyostelium discoideum (Social amoeba).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Plays a role in pre-mRNA splicing as component of the U4/U6-
CC U5 tri-snRNP complex that is involved in spliceosome assembly, and as
CC component of the precatalytic spliceosome (spliceosome B complex). The
CC heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract
CC of U6 snRNA. {ECO:0000250|UniProtKB:Q9Y4Z0}.
CC -!- SUBUNIT: Component of the precatalytic spliceosome (spliceosome B
CC complex). Component of the U4/U6-U5 tri-snRNP complex, a building block
CC of the precatalytic spliceosome (spliceosome B complex). LSM2, LSM3,
CC LSM4, LSM5, LSM6, LSM7 and LSM8 form a heptameric, ring-shaped
CC subcomplex (the LSM2-8 complex) that is part of the U4/U6-U5 tri-snRNP
CC complex and the precatalytic spliceosome.
CC {ECO:0000250|UniProtKB:Q9Y4Z0}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9Y4Z0}.
CC -!- SIMILARITY: Belongs to the snRNP Sm proteins family. {ECO:0000305}.
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DR EMBL; AAFI02000096; EAL63891.1; -; Genomic_DNA.
DR RefSeq; XP_637396.1; XM_632304.1.
DR AlphaFoldDB; Q54KX4; -.
DR SMR; Q54KX4; -.
DR STRING; 44689.Q54KX4; -.
DR PaxDb; 44689-DDB0233378; -.
DR EnsemblProtists; EAL63891; EAL63891; DDB_G0287053.
DR GeneID; 8625927; -.
DR KEGG; ddi:DDB_G0287053; -.
DR dictyBase; DDB_G0287053; lsm4.
DR eggNOG; KOG3293; Eukaryota.
DR HOGENOM; CLU_099537_2_1_1; -.
DR InParanoid; Q54KX4; -.
DR OMA; VACDAWM; -.
DR PhylomeDB; Q54KX4; -.
DR Reactome; R-DDI-430039; mRNA decay by 5' to 3' exoribonuclease.
DR PRO; PR:Q54KX4; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0005730; C:nucleolus; ISS:dictyBase.
DR GO; GO:0000932; C:P-body; IBA:GO_Central.
DR GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
DR GO; GO:0097526; C:spliceosomal tri-snRNP complex; IBA:GO_Central.
DR GO; GO:0046540; C:U4/U6 x U5 tri-snRNP complex; ISS:dictyBase.
DR GO; GO:0005688; C:U6 snRNP; ISS:dictyBase.
DR GO; GO:0003723; F:RNA binding; ISS:dictyBase.
DR GO; GO:0017070; F:U6 snRNA binding; IBA:GO_Central.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:dictyBase.
DR GO; GO:0000956; P:nuclear-transcribed mRNA catabolic process; IEA:InterPro.
DR GO; GO:0033962; P:P-body assembly; IBA:GO_Central.
DR GO; GO:0000387; P:spliceosomal snRNP assembly; IBA:GO_Central.
DR CDD; cd01723; LSm4; 1.
DR Gene3D; 2.30.30.100; -; 1.
DR InterPro; IPR034101; Lsm4.
DR InterPro; IPR027141; LSm4/Sm_D1/D3.
DR InterPro; IPR010920; LSM_dom_sf.
DR InterPro; IPR047575; Sm.
DR InterPro; IPR001163; Sm_dom_euk/arc.
DR PANTHER; PTHR23338; SMALL NUCLEAR RIBONUCLEOPROTEIN SM; 1.
DR PANTHER; PTHR23338:SF16; U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM4; 1.
DR Pfam; PF01423; LSM; 1.
DR SMART; SM00651; Sm; 1.
DR SUPFAM; SSF50182; Sm-like ribonucleoproteins; 1.
DR PROSITE; PS52002; SM; 1.
PE 3: Inferred from homology;
KW mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW Ribonucleoprotein; RNA-binding; Spliceosome.
FT CHAIN 1..177
FT /note="Probable U6 snRNA-associated Sm-like protein LSm4"
FT /id="PRO_0000328007"
FT DOMAIN 2..75
FT /note="Sm"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01346"
FT REGION 87..177
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 177 AA; 19123 MW; 3BBCC40B56B393C8 CRC64;
MLPLSLLRTG QGHQIMVELK NGETYNGLLV NCDNWMNINL KNVIRTSKDS DKFWKIQSCY
IRGNTIKYIS VPDEIIDLVA EEEQTLRTTY QQRNDSNRGR GRGDGFAGRG RGDASGRGRG
DNSYRGGRGG GNNNSGGGDS SPAGRGRGEY RGSSRGGRGD FRGSSRGGRG GNNNSTA
//
