ID M0CF30_9EURY Unreviewed; 302 AA.
AC M0CF30;
DT 03-APR-2013, integrated into UniProtKB/TrEMBL.
DT 03-APR-2013, sequence version 1.
DT 27-MAR-2024, entry version 44.
DE RecName: Full=Probable tRNA pseudouridine synthase B {ECO:0000256|HAMAP-Rule:MF_01081};
DE EC=5.4.99.25 {ECO:0000256|HAMAP-Rule:MF_01081};
DE AltName: Full=tRNA pseudouridine(55) synthase {ECO:0000256|HAMAP-Rule:MF_01081};
DE Short=Psi55 synthase {ECO:0000256|HAMAP-Rule:MF_01081};
DE AltName: Full=tRNA pseudouridylate synthase {ECO:0000256|HAMAP-Rule:MF_01081};
DE AltName: Full=tRNA-uridine isomerase {ECO:0000256|HAMAP-Rule:MF_01081};
GN Name=truB {ECO:0000256|HAMAP-Rule:MF_01081};
GN ORFNames=C476_07658 {ECO:0000313|EMBL:ELZ21865.1};
OS Natrinema limicola JCM 13563.
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Natrialbales;
OC Natrialbaceae; Natrinema.
OX NCBI_TaxID=1230457 {ECO:0000313|EMBL:ELZ21865.1, ECO:0000313|Proteomes:UP000011615};
RN [1] {ECO:0000313|EMBL:ELZ21865.1, ECO:0000313|Proteomes:UP000011615}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 13563 {ECO:0000313|EMBL:ELZ21865.1,
RC ECO:0000313|Proteomes:UP000011615};
RX PubMed=25393412; DOI=10.1371/journal.pgen.1004784;
RA Becker E.A., Seitzer P.M., Tritt A., Larsen D., Krusor M., Yao A.I., Wu D.,
RA Madern D., Eisen J.A., Darling A.E., Facciotti M.T.;
RT "Phylogenetically driven sequencing of extremely halophilic archaea reveals
RT strategies for static and dynamic osmo-response.";
RL PLoS Genet. 10:E1004784-E1004784(2014).
CC -!- FUNCTION: Could be responsible for synthesis of pseudouridine from
CC uracil-55 in the psi GC loop of transfer RNAs. {ECO:0000256|HAMAP-
CC Rule:MF_01081}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01081};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family. Type 2
CC subfamily. {ECO:0000256|HAMAP-Rule:MF_01081}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ELZ21865.1}.
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DR EMBL; AOIT01000030; ELZ21865.1; -; Genomic_DNA.
DR RefSeq; WP_008011554.1; NZ_AOIT01000030.1.
DR AlphaFoldDB; M0CF30; -.
DR STRING; 1230457.C476_07658; -.
DR PATRIC; fig|1230457.4.peg.1538; -.
DR eggNOG; arCOG00987; Archaea.
DR OrthoDB; 35866at2157; -.
DR Proteomes; UP000011615; Unassembled WGS sequence.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR CDD; cd21148; PUA_Cbf5; 1.
DR Gene3D; 3.30.2350.10; Pseudouridine synthase; 1.
DR Gene3D; 2.30.130.10; PUA domain; 1.
DR HAMAP; MF_01081; TruB_arch; 1.
DR InterPro; IPR012960; Dyskerin-like.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR002501; PsdUridine_synth_N.
DR InterPro; IPR002478; PUA.
DR InterPro; IPR015947; PUA-like_sf.
DR InterPro; IPR036974; PUA_sf.
DR InterPro; IPR004802; tRNA_PsdUridine_synth_B_fam.
DR InterPro; IPR026326; TruB_arch.
DR InterPro; IPR032819; TruB_C.
DR NCBIfam; TIGR00425; CBF5; 1.
DR PANTHER; PTHR23127; CENTROMERE/MICROTUBULE BINDING PROTEIN CBF5; 1.
DR PANTHER; PTHR23127:SF0; H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT DKC1; 1.
DR Pfam; PF08068; DKCLD; 1.
DR Pfam; PF01472; PUA; 1.
DR Pfam; PF16198; TruB_C_2; 1.
DR Pfam; PF01509; TruB_N; 2.
DR SMART; SM01136; DKCLD; 1.
DR SMART; SM00359; PUA; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR SUPFAM; SSF88697; PUA domain-like; 1.
DR PROSITE; PS50890; PUA; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|HAMAP-Rule:MF_01081};
KW Reference proteome {ECO:0000313|Proteomes:UP000011615};
KW tRNA processing {ECO:0000256|HAMAP-Rule:MF_01081}.
FT DOMAIN 1..35
FT /note="Dyskerin-like"
FT /evidence="ECO:0000259|SMART:SM01136"
FT DOMAIN 232..302
FT /note="PUA"
FT /evidence="ECO:0000259|SMART:SM00359"
FT ACT_SITE 65
FT /note="Nucleophile"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01081"
SQ SEQUENCE 302 AA; 32711 MW; 7F931EC2516C93F7 CRC64;
MPLRGPPEER SPTELLTFGV VNLDKPPGPS SHQVSGWLRD AVDETLADCG ADVTIDRAAH
AGTLDPKVTG CLPIMLGDAT RLAPVFLEGA KEYVAVLECH APVPADAESV VAEFEGPIYQ
KPPRKSAVSR RLRVREIYDL EVLEAEERRL LVRIRCESGT YVRKLCHDLG LALGTGAHMG
HLRRTMTEPF DDRTLHSAHD FLDALAFWLE DDDPDPLYEV VDPAERILEG IPSVVIAESA
AREVAEGAPV YAPGVLDADD DIERDSLVAC YTPNGAAVCL GTFVGDLEAD SGLAVDLERV
LV
//
