ID M0EA94_9EURY Unreviewed; 269 AA.
AC M0EA94;
DT 03-APR-2013, integrated into UniProtKB/TrEMBL.
DT 03-APR-2013, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=Diadenylate cyclase {ECO:0000256|HAMAP-Rule:MF_00840};
DE Short=DAC {ECO:0000256|HAMAP-Rule:MF_00840};
DE EC=2.7.7.85 {ECO:0000256|HAMAP-Rule:MF_00840};
DE AltName: Full=Cyclic-di-AMP synthase {ECO:0000256|HAMAP-Rule:MF_00840};
DE Short=c-di-AMP synthase {ECO:0000256|HAMAP-Rule:MF_00840};
GN Name=dacZ {ECO:0000256|HAMAP-Rule:MF_00840};
GN ORFNames=C463_06942 {ECO:0000313|EMBL:ELZ44675.1};
OS Halorubrum californiense DSM 19288.
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC Halorubraceae; Halorubrum.
OX NCBI_TaxID=1227465 {ECO:0000313|EMBL:ELZ44675.1, ECO:0000313|Proteomes:UP000011586};
RN [1] {ECO:0000313|EMBL:ELZ44675.1, ECO:0000313|Proteomes:UP000011586}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 19288 {ECO:0000313|EMBL:ELZ44675.1,
RC ECO:0000313|Proteomes:UP000011586};
RX PubMed=25393412; DOI=10.1371/journal.pgen.1004784;
RA Becker E.A., Seitzer P.M., Tritt A., Larsen D., Krusor M., Yao A.I., Wu D.,
RA Madern D., Eisen J.A., Darling A.E., Facciotti M.T.;
RT "Phylogenetically driven sequencing of extremely halophilic archaea reveals
RT strategies for static and dynamic osmo-response.";
RL PLoS Genet. 10:E1004784-E1004784(2014).
CC -!- FUNCTION: Diadenylate cyclase that catalyzes the condensation of 2 ATP
CC molecules into cyclic di-AMP (c-di-AMP). c-di-AMP is a second messenger
CC for intracellular signal transduction involved in the control of
CC important regulatory processes such as osmoregulation.
CC {ECO:0000256|HAMAP-Rule:MF_00840}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 ATP = 3',3'-c-di-AMP + 2 diphosphate; Xref=Rhea:RHEA:35655,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:71500; EC=2.7.7.85;
CC Evidence={ECO:0000256|ARBA:ARBA00000877, ECO:0000256|HAMAP-
CC Rule:MF_00840};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_00840};
CC -!- SIMILARITY: Belongs to the adenylate cyclase family. DacZ subfamily.
CC {ECO:0000256|HAMAP-Rule:MF_00840}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ELZ44675.1}.
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DR EMBL; AOJK01000036; ELZ44675.1; -; Genomic_DNA.
DR RefSeq; WP_008442302.1; NZ_AOJK01000036.1.
DR AlphaFoldDB; M0EA94; -.
DR STRING; 1227465.C463_06942; -.
DR PATRIC; fig|1227465.4.peg.1357; -.
DR OrthoDB; 85944at2157; -.
DR Proteomes; UP000011586; Unassembled WGS sequence.
DR GO; GO:0004016; F:adenylate cyclase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0106408; F:diadenylate cyclase activity; IEA:UniProtKB-EC.
DR GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0019932; P:second-messenger-mediated signaling; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.1700.10; DNA integrity scanning protein, DisA, N-terminal domain; 1.
DR HAMAP; MF_00840; DacZ; 1.
DR InterPro; IPR014499; DAC_DacZ.
DR InterPro; IPR045586; DacZ_N.
DR InterPro; IPR036888; DNA_integrity_DisA_N_sf.
DR InterPro; IPR003390; DNA_integrity_scan_DisA_N.
DR NCBIfam; NF041371; Diadnylcyc_Halo; 1.
DR PANTHER; PTHR34185; DIADENYLATE CYCLASE; 1.
DR PANTHER; PTHR34185:SF1; DIADENYLATE CYCLASE; 1.
DR Pfam; PF02457; DAC; 1.
DR Pfam; PF19294; DACZ_N; 1.
DR PIRSF; PIRSF019073; UCP019073; 1.
DR SUPFAM; SSF143597; YojJ-like; 1.
DR PROSITE; PS51794; DAC; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00840}; Manganese {ECO:0000256|HAMAP-Rule:MF_00840};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00840};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695, ECO:0000256|HAMAP-
KW Rule:MF_00840};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_00840}.
FT DOMAIN 109..266
FT /note="DAC"
FT /evidence="ECO:0000259|PROSITE:PS51794"
SQ SEQUENCE 269 AA; 28698 MW; AA1AE9E986605BD5 CRC64;
MASLTDHLAD LVAEVDAALL FSPTSSFYER FADDGSEVVV VAPDNDVDAE TFVDLPLPFD
NVKDRIRFGI EGAMDADLVS EGDEVACVAS VFDGGSDAVI RATVDQGVHT GIYDLFANSR
AEPSVIRDVF EVAIELGQKG QKGKPVGALF VVGDAGKVMN KSRPLSYNPF EKSHVHVGDP
IVNVMLKEFS RLDGAFVVSD SGKIVSAYRY LEPAAEGVDI PKGLGARHMS GAAITRDTNS
TAIVLSESDG LVRAFKAGEL VLEIDPEEY
//
