ID M0N3D3_9EURY Unreviewed; 915 AA.
AC M0N3D3;
DT 03-APR-2013, integrated into UniProtKB/TrEMBL.
DT 03-APR-2013, sequence version 1.
DT 27-MAR-2024, entry version 45.
DE RecName: Full=DNA helicase {ECO:0000256|ARBA:ARBA00012551};
DE EC=3.6.4.12 {ECO:0000256|ARBA:ARBA00012551};
GN ORFNames=C450_10133 {ECO:0000313|EMBL:EMA52452.1};
OS Halococcus salifodinae DSM 8989.
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC Halococcaceae; Halococcus.
OX NCBI_TaxID=1227456 {ECO:0000313|EMBL:EMA52452.1, ECO:0000313|Proteomes:UP000011625};
RN [1] {ECO:0000313|EMBL:EMA52452.1, ECO:0000313|Proteomes:UP000011625}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 8989 {ECO:0000313|EMBL:EMA52452.1,
RC ECO:0000313|Proteomes:UP000011625};
RX PubMed=25393412; DOI=10.1371/journal.pgen.1004784;
RA Becker E.A., Seitzer P.M., Tritt A., Larsen D., Krusor M., Yao A.I., Wu D.,
RA Madern D., Eisen J.A., Darling A.E., Facciotti M.T.;
RT "Phylogenetically driven sequencing of extremely halophilic archaea reveals
RT strategies for static and dynamic osmo-response.";
RL PLoS Genet. 10:E1004784-E1004784(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|ARBA:ARBA00001665};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the DNA2/NAM7 helicase family.
CC {ECO:0000256|ARBA:ARBA00007913}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EMA52452.1}.
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DR EMBL; AOME01000054; EMA52452.1; -; Genomic_DNA.
DR RefSeq; WP_005043140.1; NZ_AOME01000054.1.
DR AlphaFoldDB; M0N3D3; -.
DR STRING; 1227456.C450_10133; -.
DR PATRIC; fig|1227456.3.peg.2048; -.
DR OrthoDB; 45637at2157; -.
DR Proteomes; UP000011625; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd18808; SF1_C_Upf1; 1.
DR Gene3D; 2.40.30.270; -; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR041679; DNA2/NAM7-like_C.
DR InterPro; IPR041677; DNA2/NAM7_AAA_11.
DR InterPro; IPR014808; DNA_replication_fac_Dna2_N.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR047187; SF1_C_Upf1.
DR PANTHER; PTHR43788; DNA2/NAM7 HELICASE FAMILY MEMBER; 1.
DR PANTHER; PTHR43788:SF8; HELICASE WITH ZINC FINGER 2; 1.
DR Pfam; PF13086; AAA_11; 2.
DR Pfam; PF13087; AAA_12; 1.
DR Pfam; PF08696; Dna2; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022806};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022485};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741}.
FT DOMAIN 40..184
FT /note="DNA replication factor Dna2 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF08696"
FT DOMAIN 511..602
FT /note="DNA2/NAM7 helicase helicase"
FT /evidence="ECO:0000259|Pfam:PF13086"
FT DOMAIN 611..676
FT /note="DNA2/NAM7 helicase helicase"
FT /evidence="ECO:0000259|Pfam:PF13086"
FT DOMAIN 713..898
FT /note="DNA2/NAM7 helicase-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF13087"
SQ SEQUENCE 915 AA; 99862 MW; 29C201B0205A9C80 CRC64;
MAELRGPIVD VGETRSVSTQ YGERDLAELT LRPERGQAEP VTLTLWGKWS EIAEYAQTGM
EVLALGVEHE EFRGEIQYAT AGDSRVVIEP DYLVNVTDVR SWVQCPRMHY LNTLSGVPLN
YPVVKGTIVH EVFSDLLRGG EVEAAIDDRV AEVGLDLGLL DRDADEVRED VSDHARAIDG
WLSQGTLDGG DEWRSEQTLI SERYGLKGRA DAVRRGAPVE LKTGKNTNSE PRFQDKVQVA
CYALLLDERG EPPDTGTLLY TKNAALDRNE ESGDLSPTKD FSLNDGLLDY VLRERNHLAA
MAVAGEIPTG YEANATCNYC FEQDTCMVIS GRLDQESKAG QIGEAIPEGE RAYFDRFYRA
IEAERGAVHN EYAKLWTQTA TERADADRAL IDLEPAGEIE VDGRWELRAR CDDPVSKIRE
GDVVLASDGH PVRGNAEMAR VVSMGEEIVV TADEPVELRR LDVYPSEIGA DRMLTALHDA
LLAGDESQKD VLFDRRDPAF QDIEETFIDN NPAQDRAVRR AVGAEDCALI HGPPGTGKTY
TIARTVRALV ERGERVLLSA FTNRAVDNAL DALRDQGFEE FVRVGTEHGV REDIQEHRLE
SRGDPDERAA ELRNAPVVAA TTASCGSRIM REGSFDVAVI DEASQLTEPA TLAAVTLADR
FVLVGDHQQL PPVVQSADAT GTVETTTATD GSGATAAKTM DGAGGATSGI ADLSRSLFER
LIETHPEAAV LLDTQYRMAQ RIQAFSSDEF YDGRLRPASP AVAGQHVSDL PGVTSDALPD
HLQERVTFVD PDGTVRGNTN PAEVEAVAEI VEAYASAGVA RTDIGVIAPF RAQAAAIRRE
VPDVTVDTVD RFQGSAKEII IVSFVATGDL DSPIFEDFRR VNVALTRAKK GLVLVGDRAA
LATDERYARM VEWAE
//
