ID M1V553_CYAM1 Unreviewed; 1775 AA.
AC M1V553;
DT 01-MAY-2013, integrated into UniProtKB/TrEMBL.
DT 01-MAY-2013, sequence version 1.
DT 27-MAR-2024, entry version 54.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN ORFNames=CYME_CMI198C {ECO:0000313|EMBL:BAM80070.1};
OS Cyanidioschyzon merolae (strain NIES-3377 / 10D) (Unicellular red alga).
OC Eukaryota; Rhodophyta; Bangiophyceae; Cyanidiales; Cyanidiaceae;
OC Cyanidioschyzon.
OX NCBI_TaxID=280699 {ECO:0000313|EMBL:BAM80070.1, ECO:0000313|Proteomes:UP000007014};
RN [1] {ECO:0000313|EMBL:BAM80070.1, ECO:0000313|Proteomes:UP000007014}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=10D {ECO:0000313|EMBL:BAM80070.1,
RC ECO:0000313|Proteomes:UP000007014};
RX PubMed=15071595; DOI=10.1038/nature02398;
RA Matsuzaki M., Misumi O., Shin-i T., Maruyama S., Takahara M.,
RA Miyagishima S., Mori T., Nishida K., Yagisawa F., Nishida K., Yoshida Y.,
RA Nishimura Y., Nakao S., Kobayashi T., Momoyama Y., Higashiyama T.,
RA Minoda A., Sano M., Nomoto H., Oishi K., Hayashi H., Ohta F., Nishizaka S.,
RA Haga S., Miura S., Morishita T., Kabeya Y., Terasawa K., Suzuki Y.,
RA Ishii Y., Asakawa S., Takano H., Ohta N., Kuroiwa H., Tanaka K.,
RA Shimizu N., Sugano S., Sato N., Nozaki H., Ogasawara N., Kohara Y.,
RA Kuroiwa T.;
RT "Genome sequence of the ultrasmall unicellular red alga Cyanidioschyzon
RT merolae 10D.";
RL Nature 428:653-657(2004).
RN [2] {ECO:0000313|EMBL:BAM80070.1, ECO:0000313|Proteomes:UP000007014}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=10D {ECO:0000313|EMBL:BAM80070.1,
RC ECO:0000313|Proteomes:UP000007014};
RX PubMed=17623057; DOI=10.1186/1741-7007-5-28;
RA Nozaki H., Takano H., Misumi O., Terasawa K., Matsuzaki M., Maruyama S.,
RA Nishida K., Yagisawa F., Yoshida Y., Fujiwara T., Takio S., Tamura K.,
RA Chung S.J., Nakamura S., Kuroiwa H., Tanaka K., Sato N., Kuroiwa T.;
RT "A 100%-complete sequence reveals unusually simple genomic features in the
RT hot-spring red alga Cyanidioschyzon merolae.";
RL BMC Biol. 5:28-28(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC -!- SIMILARITY: Belongs to the UPL family. K-HECT subfamily.
CC {ECO:0000256|ARBA:ARBA00006331}.
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DR EMBL; AP006491; BAM80070.1; -; Genomic_DNA.
DR RefSeq; XP_005536356.1; XM_005536299.1.
DR STRING; 280699.M1V553; -.
DR EnsemblPlants; CMI198CT; CMI198CT; CMI198C.
DR GeneID; 16993616; -.
DR Gramene; CMI198CT; CMI198CT; CMI198C.
DR KEGG; cme:CYME_CMI198C; -.
DR eggNOG; KOG0168; Eukaryota.
DR eggNOG; KOG0170; Eukaryota.
DR HOGENOM; CLU_000366_1_1_1; -.
DR OMA; AEPLSQF; -.
DR OrthoDB; 1093891at2759; -.
DR Proteomes; UP000007014; Chromosome 9.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:InterPro.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR045322; HECTD1/TRIP12-like.
DR PANTHER; PTHR45670; E3 UBIQUITIN-PROTEIN LIGASE TRIP12; 1.
DR PANTHER; PTHR45670:SF1; E3 UBIQUITIN-PROTEIN LIGASE TRIP12; 1.
DR Pfam; PF00632; HECT; 1.
DR SMART; SM00119; HECTc; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR PROSITE; PS50237; HECT; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000007014};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 1378..1775
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 1..234
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 898..1085
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1321..1351
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 82..117
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 911..927
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 984..999
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1071..1085
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1321..1342
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 1742
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 1775 AA; 193985 MW; 1EE89B1ADB49EFE9 CRC64;
MARGRRRSAL SRAQQHVERN TGAREETVTE GANVAKRAAG LARQEAETTT VESGQHAGAV
ESAALEKTEV ARNTQRAVES QALDGHRYRK RRSACLEKEP VGRVLETRSE SKIAAEEPHA
ATTRQGSKRM RRSRSRQRAL REATAGMLGG SDSTGADNDG LSSINSSESR SRRRYHSRYR
RHTEPNTSAS LPLETNDDIP EASSLGFPLE HQSGRRRGAS PRGPSSLPPE TGGTLQGLLR
HLGAGLSEIL PSFGHGGFGG SSVPARLRRI ASELRSLTTE GNTETDTVAL MALLNELCEV
LSIGVEDVIL NFSFEQLVSP LVWILQNCPE LPELLILAAR ALAYMVEISP SVGASIAQSR
AVPALCGFLL HVEYIDLAEQ CLQALERLSY NFPNHIVRSG GLRAILQHLD FFPTSIQRMA
SSAAANCCAR VNDSALSQVE DALELLSHLL DSDDTRIREH GVTAYARLVA NFSASSNAQE
VLRRIANDGG AMEKLVAVLA AGEASLDKRH RGLVLSTLSS LASADEGLGT RMLTSPAQGG
LGIGAILANL LGVASDAEES TSTGQLLGAA DVHAVPLNRA TQTETLIGSA LMLVDALAPD
RLLVQRLQTV LELNVRAPMH DQTSREGRRR AISAMKKTLF ALESEQEIQS FSMQLVPTLG
YVLYDPIDED ERASVMCMIE HMLERVQDLG PLGELFVKEG VLHVLEQSAV ETDAAQKILD
RFGDTLRSSG RQRIRKLCEL VERIKKGDHE SLIALFPELE HCSVFEVQMC GLAGALDAYL
SVQPGAASEL ATRYSHLLVA AIRAPGALRR LVRSLVAIFT NTERFQVVRN MAANGSVSAG
IRLLSQPFRL QLQRGGGCSG SRLRDFPASL VLVEPLASAQ TIEEFLYERV TGDAAELNEL
SDEPVFETGT DENGSDDSGA FEVATESTAD TSDDEHAIFF DEDALSGSVE SDEPGEHRTR
RRQAGSRPSL AGETALAAAE ETASGSEEDS DEFSDLGETF DELGPEHGLE NPNLSGSLPA
VDIEFSDEVS GTGSPVREQE HSGRDSRRSA SETYAARAGR CAGAPCPSSE IDRGRRSRGI
RLKRDGERRR ARLSLCIRGH TLLPQETILH ALVTTLGGQA LGPRLWSETH ALTYADCQSS
RMGQVSSQPI SNGDGKPGGH QFEGPFAEFE LVQVAELQFQ VPRSFHLNEV TVAQDVSSYL
SLLGKLYWMN NHHAELHAWI QASRTQSGST LDAAWSHNVH WPPADDPLVP EADIFASSSL
ENKLGAQLSD PLAVAAAALP PWCFIVPRVC PGLFSLQTRR LLVQVSSFGI ARALSAVQTR
ADTQRQQHAT ASSSTGRSDL GAHTSGAEQE ARIGRIQRQK VRVHRARLLE SAELIMENFA
DQKALIEVEY FNEAGTGLGP TLEFYSMVSR ELQRVDLKLW WEDSSKAAER ERLLRSGKAL
PPELRERLCY VVSAGAGLYP APIGPRERGA PVQERLQRFQ FIGRFCAKAF LDGRLLDLRF
SPAFWRLVRA LAEARFLVSE GLNGHRAIFR AAKKRLDFSL RRLGEVDAEL ARSLHSIACM
RDADACDIAA LCLDWTLPGQ PHIEMRPRGR SMTVSKDNLA DYVRQVKEHV LFDCAARPAY
AFLEGFQSIC SIWALLGIFN PNEEVNVVLC GPDLHPWTEQ ELLSALRFDH GYTSENVAAR
NFVRALCSLN EDDRRHFLLF ATGSPRLPMG GFHALLPPMT VVKRTPEAGL TPDECLPTVM
TCTNYVKLPE YSSFEILLDR LLYTIREGQN SFDLS
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