ID M3MTN1_HELPX Unreviewed; 326 AA.
AC M3MTN1;
DT 01-MAY-2013, integrated into UniProtKB/TrEMBL.
DT 01-MAY-2013, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=Nitrogen fixation protein NifU {ECO:0000256|ARBA:ARBA00015278, ECO:0000256|PIRNR:PIRNR000375};
GN ORFNames=HMPREF1402_01072 {ECO:0000313|EMBL:EMG97839.1};
OS Helicobacter pylori GAM121Aii.
OC Bacteria; Campylobacterota; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=1159030 {ECO:0000313|EMBL:EMG97839.1, ECO:0000313|Proteomes:UP000011875};
RN [1] {ECO:0000313|EMBL:EMG97839.1, ECO:0000313|Proteomes:UP000011875}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GAM121Aii {ECO:0000313|EMBL:EMG97839.1,
RC ECO:0000313|Proteomes:UP000011875};
RA Weinstock G., Sodergren E., Lobos E.A., Fulton L., Fulton R., Courtney L.,
RA Fronick C., O'Laughlin M., Godfrey J., Wilson R.M., Miner T., Farmer C.,
RA Delehaunty K., Cordes M., Minx P., Tomlinson C., Chen J., Wollam A.,
RA Pepin K.H., Bhonagiri V., Zhang X., Suruliraj S., Antonio M., Secka O.,
RA Thomas J., Warren W., Mitreva M., Mardis E.R., Wilson R.K.;
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: May be involved in the formation or repair of [Fe-S] clusters
CC present in iron-sulfur proteins. {ECO:0000256|PIRNR:PIRNR000375}.
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000256|PIRSR:PIRSR000375-1};
CC Note=Binds 1 Fe cation per subunit. {ECO:0000256|PIRSR:PIRSR000375-1};
CC -!- COFACTOR:
CC Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC Evidence={ECO:0000256|PIRSR:PIRSR000375-1};
CC Note=Binds 1 [2Fe-2S] cluster per subunit.
CC {ECO:0000256|PIRSR:PIRSR000375-1};
CC -!- SIMILARITY: Belongs to the NifU family. {ECO:0000256|ARBA:ARBA00006420,
CC ECO:0000256|PIRNR:PIRNR000375}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EMG97839.1}.
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DR EMBL; APDG01000055; EMG97839.1; -; Genomic_DNA.
DR RefSeq; WP_001929288.1; NZ_KB636372.1.
DR AlphaFoldDB; M3MTN1; -.
DR PATRIC; fig|1159030.3.peg.999; -.
DR HOGENOM; CLU_079283_0_0_7; -.
DR Proteomes; UP000011875; Unassembled WGS sequence.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0016226; P:iron-sulfur cluster assembly; IEA:InterPro.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR CDD; cd06664; IscU_like; 1.
DR Gene3D; 3.90.1010.10; -; 1.
DR Gene3D; 1.10.10.1100; BFD-like [2Fe-2S]-binding domain; 1.
DR Gene3D; 3.30.300.130; Fe-S cluster assembly (FSCA); 1.
DR InterPro; IPR007419; BFD-like_2Fe2S-bd_dom.
DR InterPro; IPR041854; BFD-like_2Fe2S-bd_dom_sf.
DR InterPro; IPR034904; FSCA_dom_sf.
DR InterPro; IPR016217; N_fixation_NifU.
DR InterPro; IPR001075; NIF_FeS_clus_asmbl_NifU_C.
DR InterPro; IPR002871; NIF_FeS_clus_asmbl_NifU_N.
DR PANTHER; PTHR10093:SF8; IRON-SULFUR CLUSTER ASSEMBLY ENZYME ISCU, MITOCHONDRIAL; 1.
DR PANTHER; PTHR10093; IRON-SULFUR CLUSTER ASSEMBLY ENZYME NIFU HOMOLOG; 1.
DR Pfam; PF04324; Fer2_BFD; 1.
DR Pfam; PF01106; NifU; 1.
DR Pfam; PF01592; NifU_N; 1.
DR PIRSF; PIRSF000375; NifU; 1.
DR SUPFAM; SSF117916; Fe-S cluster assembly (FSCA) domain-like; 1.
DR SUPFAM; SSF82649; SufE/NifU; 1.
PE 3: Inferred from homology;
KW 2Fe-2S {ECO:0000256|PIRSR:PIRSR000375-1};
KW Iron {ECO:0000256|PIRSR:PIRSR000375-1};
KW Iron-sulfur {ECO:0000256|PIRSR:PIRSR000375-1};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR000375-1};
KW Nitrogen fixation {ECO:0000256|ARBA:ARBA00023231,
KW ECO:0000256|PIRNR:PIRNR000375}.
FT DOMAIN 14..151
FT /note="NIF system FeS cluster assembly NifU N-terminal"
FT /evidence="ECO:0000259|Pfam:PF01592"
FT DOMAIN 159..201
FT /note="BFD-like [2Fe-2S]-binding"
FT /evidence="ECO:0000259|Pfam:PF04324"
FT DOMAIN 254..323
FT /note="NIF system FeS cluster assembly NifU C-terminal"
FT /evidence="ECO:0000259|Pfam:PF01106"
FT BINDING 54
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 81
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 131
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 161
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 163
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 196
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 199
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
SQ SEQUENCE 326 AA; 36337 MW; 3417DECFC8C76AFD CRC64;
MAKHDLVGSA LWDAYSKEVQ RRMDNPTHLG VITEEQAKAK NAKLIVADYG AEACGDAVRL
YWLVDENTDT IIDAKFKSFG CGTAIASSDM MVELCLNKRV QDAVKITNLD VERGLRDDPD
TPAVPGQKMH CSVMAYDVIK KAAGMYLGKN AEDFEEEIIV CECARVSLGT IKEVIRLNDL
KSVEEITNYT KAGAFCKSCV RPGGHEKRDY YLVDILKEVR EEMEAEKLKA TANKSQNGEL
AFREMTMVQK IKAVDKVIDE NIRAMLMMDG GDLEILDIKE SDDYIDVYIR YMGACDGCMS
ATTGTLFAIE NALQELLDRS IRVLPI
//
