ID M4RCB4_9BIFI Unreviewed; 443 AA.
AC M4RCB4;
DT 29-MAY-2013, integrated into UniProtKB/TrEMBL.
DT 29-MAY-2013, sequence version 1.
DT 24-JAN-2024, entry version 46.
DE SubName: Full=DNA-directed DNA polymerase {ECO:0000313|EMBL:AGH41131.1};
GN ORFNames=D805_0864 {ECO:0000313|EMBL:AGH41131.1};
OS Bifidobacterium thermophilum RBL67.
OC Bacteria; Actinomycetota; Actinomycetes; Bifidobacteriales;
OC Bifidobacteriaceae; Bifidobacterium.
OX NCBI_TaxID=1254439 {ECO:0000313|EMBL:AGH41131.1, ECO:0000313|Proteomes:UP000011835};
RN [1] {ECO:0000313|EMBL:AGH41131.1, ECO:0000313|Proteomes:UP000011835}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RBL67 {ECO:0000313|EMBL:AGH41131.1,
RC ECO:0000313|Proteomes:UP000011835};
RX PubMed=23640377;
RA Jans C., Lacroix C., Follador R., Stevens M.J.;
RT "Complete Genome Sequence of the Probiotic Bifidobacterium thermophilum
RT Strain RBL67.";
RL Genome Announc. 1:E00191-13(2013).
CC -!- FUNCTION: Poorly processive, error-prone DNA polymerase involved in
CC untargeted mutagenesis. Copies undamaged DNA at stalled replication
CC forks, which arise in vivo from mismatched or misaligned primer ends.
CC These misaligned primers can be extended by PolIV. Exhibits no 3'-5'
CC exonuclease (proofreading) activity. May be involved in translesional
CC synthesis, in conjunction with the beta clamp from PolIII.
CC {ECO:0000256|ARBA:ARBA00025589}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-Y family.
CC {ECO:0000256|ARBA:ARBA00010945}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP004346; AGH41131.1; -; Genomic_DNA.
DR RefSeq; WP_015450390.1; NC_020546.1.
DR AlphaFoldDB; M4RCB4; -.
DR KEGG; btp:D805_0864; -.
DR PATRIC; fig|1254439.12.peg.858; -.
DR HOGENOM; CLU_012348_3_0_11; -.
DR Proteomes; UP000011835; Chromosome.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR GO; GO:0009432; P:SOS response; IEA:UniProtKB-KW.
DR CDD; cd01700; PolY_Pol_V_umuC; 1.
DR Gene3D; 3.30.70.270; -; 1.
DR Gene3D; 3.40.1170.60; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR017961; DNA_pol_Y-fam_little_finger.
DR InterPro; IPR025188; DUF4113.
DR InterPro; IPR024728; PolY_HhH_motif.
DR InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR InterPro; IPR001126; UmuC.
DR PANTHER; PTHR11076; DNA REPAIR POLYMERASE UMUC / TRANSFERASE FAMILY MEMBER; 1.
DR PANTHER; PTHR11076:SF34; PROTEIN UMUC; 1.
DR Pfam; PF13438; DUF4113; 1.
DR Pfam; PF00817; IMS; 1.
DR Pfam; PF11799; IMS_C; 1.
DR Pfam; PF11798; IMS_HHH; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR PROSITE; PS50173; UMUC; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00023199};
KW DNA-directed DNA polymerase {ECO:0000313|EMBL:AGH41131.1};
KW Nucleotidyltransferase {ECO:0000313|EMBL:AGH41131.1};
KW SOS mutagenesis {ECO:0000256|ARBA:ARBA00023199};
KW SOS response {ECO:0000256|ARBA:ARBA00023236};
KW Transferase {ECO:0000313|EMBL:AGH41131.1}.
FT DOMAIN 11..197
FT /note="UmuC"
FT /evidence="ECO:0000259|PROSITE:PS50173"
SQ SEQUENCE 443 AA; 48718 MW; 63CCAFA948CE58BF CRC64;
MPDPRVQEHP IVLADANNFY ASCEAVFHPE VAGKPLVVLS NNDGCVVSRS YEAKALGIAN
GTPWFTIRQE AAYDQVIARS SNYELYGSLS KRMMSIMSGF LPGQEVYSID ECFMHSPWDS
EQTRRIAAVM RRAVLQGVGI PVSIGIAPTK TLAKITNHWA KRHPSSRGVT TWGDLSRDDQ
GTAVLSSIEI DDVWGIGRRL AKRLRGLGIR QAAQLRDQDP VNMRRRFSVQ LERTILELRG
IPCIADDSDA TGDKRDCQIL CSRMFGTPIL GEEGLRQAAS VYAQYACRRL QRQHSLCTRV
SVFCASSPFS KHDQYSSVCG SILLQDPSDD PVIISAAARQ ALHGRITPSV RYVRVGVMLT
GLVDAASYDT LPALGARTDS GLSEVFEQVA RRFGPAHVGI GFAGIRGDGR NNDGTGAAWA
MRREMLSSRC TTRWDEMPVV RAD
//
