ID M5WZQ0_PRUPE Unreviewed; 1159 AA.
AC M5WZQ0;
DT 29-MAY-2013, integrated into UniProtKB/TrEMBL.
DT 29-MAY-2013, sequence version 1.
DT 27-MAR-2024, entry version 56.
DE RecName: Full=Pre-mRNA-processing ATP-dependent RNA helicase PRP5 {ECO:0000256|ARBA:ARBA00041162};
DE EC=3.6.4.13 {ECO:0000256|ARBA:ARBA00012552};
DE AltName: Full=Pre-mRNA-processing ATP-dependent RNA helicase prp5 {ECO:0000256|ARBA:ARBA00040918};
GN ORFNames=PRUPE_5G003900 {ECO:0000313|EMBL:ONI05364.1};
OS Prunus persica (Peach) (Amygdalus persica).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Amygdaleae; Prunus.
OX NCBI_TaxID=3760 {ECO:0000313|EMBL:ONI05364.1, ECO:0000313|Proteomes:UP000006882};
RN [1] {ECO:0000313|EMBL:ONI05364.1, ECO:0000313|Proteomes:UP000006882}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nemared {ECO:0000313|Proteomes:UP000006882};
RX PubMed=23525075; DOI=10.1038/ng.2586;
RA Verde I., Abbott A.G., Scalabrin S., Jung S., Shu S., Marroni F.,
RA Zhebentyayeva T., Dettori M.T., Grimwood J., Cattonaro F., Zuccolo A.,
RA Rossini L., Jenkins J., Vendramin E., Meisel L.A., Decroocq V.,
RA Sosinski B., Prochnik S., Mitros T., Policriti A., Cipriani G., Dondini L.,
RA Ficklin S., Goodstein D.M., Xuan P., Del Fabbro C., Aramini V., Copetti D.,
RA Gonzalez S., Horner D.S., Falchi R., Lucas S., Mica E., Maldonado J.,
RA Lazzari B., Bielenberg D., Pirona R., Miculan M., Barakat A., Testolin R.,
RA Stella A., Tartarini S., Tonutti P., Arus P., Orellana A., Wells C.,
RA Main D., Vizzotto G., Silva H., Salamini F., Schmutz J., Morgante M.,
RA Rokhsar D.S.;
RT "The high-quality draft genome of peach (Prunus persica) identifies unique
RT patterns of genetic diversity, domestication and genome evolution.";
RL Nat. Genet. 45:487-494(2013).
RN [2] {ECO:0000313|EMBL:ONI05364.1}
RP NUCLEOTIDE SEQUENCE.
RA Verde I., Jenkins J., Dondini L., Micali S., Pagliarani G., Vendramin E.,
RA Paris R., Aramini V., Gazza L., Rossini L., Bassi D., Troggio M., Shu S.,
RA Grimwood J.H., Tartarini S., Dettori M.T., Schmutz J.;
RT "WGS assembly of Prunus persica.";
RL Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: ATP-dependent RNA helicase involved spliceosome assembly and
CC in nuclear splicing. Catalyzes an ATP-dependent conformational change
CC of U2 snRNP. Bridges U1 and U2 snRNPs and enables stable U2 snRNP
CC association with intron RNA. {ECO:0000256|ARBA:ARBA00037330}.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX46/PRP5
CC subfamily. {ECO:0000256|ARBA:ARBA00038511}.
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DR EMBL; CM007655; ONI05359.1; -; Genomic_DNA.
DR EMBL; CM007655; ONI05360.1; -; Genomic_DNA.
DR EMBL; CM007655; ONI05361.1; -; Genomic_DNA.
DR EMBL; CM007655; ONI05362.1; -; Genomic_DNA.
DR EMBL; CM007655; ONI05363.1; -; Genomic_DNA.
DR EMBL; CM007655; ONI05364.1; -; Genomic_DNA.
DR EMBL; CM007655; ONI05365.1; -; Genomic_DNA.
DR EMBL; CM007655; ONI05366.1; -; Genomic_DNA.
DR RefSeq; XP_007211096.1; XM_007211034.1.
DR AlphaFoldDB; M5WZQ0; -.
DR SMR; M5WZQ0; -.
DR STRING; 3760.M5WZQ0; -.
DR EnsemblPlants; ONI05359; ONI05359; PRUPE_5G003900.
DR EnsemblPlants; ONI05360; ONI05360; PRUPE_5G003900.
DR EnsemblPlants; ONI05361; ONI05361; PRUPE_5G003900.
DR EnsemblPlants; ONI05362; ONI05362; PRUPE_5G003900.
DR EnsemblPlants; ONI05363; ONI05363; PRUPE_5G003900.
DR EnsemblPlants; ONI05364; ONI05364; PRUPE_5G003900.
DR EnsemblPlants; ONI05365; ONI05365; PRUPE_5G003900.
DR EnsemblPlants; ONI05366; ONI05366; PRUPE_5G003900.
DR Gramene; ONI05359; ONI05359; PRUPE_5G003900.
DR Gramene; ONI05360; ONI05360; PRUPE_5G003900.
DR Gramene; ONI05361; ONI05361; PRUPE_5G003900.
DR Gramene; ONI05362; ONI05362; PRUPE_5G003900.
DR Gramene; ONI05363; ONI05363; PRUPE_5G003900.
DR Gramene; ONI05364; ONI05364; PRUPE_5G003900.
DR Gramene; ONI05365; ONI05365; PRUPE_5G003900.
DR Gramene; ONI05366; ONI05366; PRUPE_5G003900.
DR eggNOG; KOG0334; Eukaryota.
DR eggNOG; KOG2217; Eukaryota.
DR HOGENOM; CLU_003041_0_1_1; -.
DR OMA; HSIRKRF; -.
DR OrthoDB; 5483490at2759; -.
DR Proteomes; UP000006882; Chromosome g5.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003724; F:RNA helicase activity; IEA:InterPro.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR CDD; cd17953; DEADc_DDX46; 1.
DR CDD; cd22475; KH-I_AtRH42_like; 1.
DR CDD; cd18787; SF2_C_DEAD; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR PANTHER; PTHR47958:SF35; ATP-DEPENDENT RNA HELICASE; 1.
DR PANTHER; PTHR47958; ATP-DEPENDENT RNA HELICASE DBP3; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000006882}.
FT DOMAIN 513..541
FT /note="DEAD-box RNA helicase Q"
FT /evidence="ECO:0000259|PROSITE:PS51195"
FT DOMAIN 544..722
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 750..894
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT REGION 1..338
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 382..445
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 929..956
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 513..541
FT /note="Q motif"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00552"
FT COMPBIAS 391..408
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 427..444
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 929..943
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1159 AA; 132387 MW; 7F763209BBFC380D CRC64;
MEEGKHKSRK EGESKRSHRD RQREKQRNGE RHRDKDKDKR DRDSRRSERE KSSDSEDRYD
REKHRDRDTD RTRSRDDERE RTRDRKREKD KEKEKERERA REKEKERERE REREREREKE
RDREKEREKE KEKERERERE RREREREGED REREKRERER EREKDRDRER RIRERERRRD
IDTDDTDDDV RERGRKRHRK DENEYKEKER ERSSSRSNRH RDDGDGSPRK KSDEDDSVKK
EKQPTREEEL EDEQRKLDEE MEKRRRRVQE WQELKRKKEE SEREKRGEGD VDEPKSGKAW
TLEGESDDEE VPSLGKSERD MDVDGEDNLT DREAGGDAMV VDSENETDAL TLQNGADDAV
GDEEVDPLDA FMNSMVLPEV EKLNNAVEPS IVDEKNKDKK DDLSNGEQPR RGSNKSMGRI
IPGEDSDSDY GDLENDDDPL EDEGDDEFIK RVKKTKAEKL SVVDHSKIDY DPFRKNFYIE
VKEISRMTPE QVGAYRKELE LKIHGKDVPK PIKTWHQTGL TSKILETIKK LNYEKPMPIQ
AQAVPVIMSG RDCIGIAKTG SGKTVAFVLP MLRHIKDQPP VVAGDGPIGL IMAPTRELVQ
QIHSDIKKFT KVLGLRCVPV YGGSGVAQQI SELKRGAEIV VCTPGRMIDI LCTSGGRITN
LRRVTYLVVD EADRMFDMGF EPQITRIVQN IRPDRQTVLF SATFPRQVEV LARKVLNKPV
EIQVGGRSVV NKDIAQLVEV RLENERFLRL LELLGEWYEK GKILIFVESQ NKCDALFRDL
LRHGYPCLSL HGGKDQTDRE STITDFKSNV CNLLIATSVA ARGLDVKELE LVINFDSPNH
YEDYVHRVGR TGRAGRKGCA ITFVSEEDAR YAPDLVKALE LSEQVVPDDL KSLADSFTAK
VNQGLEQAHG TGYGGSGFKF NEEEDEVRRA AKKAQAKEYG FEDDKSDSED EDEGIRKAGG
DISQQAALAQ IAAIAAASKG STASIQTPVP AAQLLPNSGL PVSLPGVLGL TLPGTAAAVA
GTGLPVVGND GAARAAAIAA AMNLQHNLAK IQADAMPEHY EAELEINDFP QNARWKVTHK
ETLGPISEWT GAAITTRGQY FPPGKVAGPG DRKLYLFIEG PTEQSVKRAK AELKRVLEDI
SNQALSLPGG AQQGRYQVL
//
