ID M7MLS8_9MICC Unreviewed; 424 AA.
AC M7MLS8;
DT 29-MAY-2013, integrated into UniProtKB/TrEMBL.
DT 29-MAY-2013, sequence version 1.
DT 24-JAN-2024, entry version 38.
DE SubName: Full=DNA polymerase IV {ECO:0000313|EMBL:EMQ97257.1};
DE EC=2.7.7.7 {ECO:0000313|EMBL:EMQ97257.1};
GN Name=polY2 {ECO:0000313|EMBL:EMQ97257.1};
GN ORFNames=ADIAG_03424 {ECO:0000313|EMBL:EMQ97257.1};
OS Paeniglutamicibacter gangotriensis Lz1y.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Micrococcaceae;
OC Paeniglutamicibacter.
OX NCBI_TaxID=1276920 {ECO:0000313|EMBL:EMQ97257.1, ECO:0000313|Proteomes:UP000012015};
RN [1] {ECO:0000313|EMBL:EMQ97257.1, ECO:0000313|Proteomes:UP000012015}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Lz1y {ECO:0000313|EMBL:EMQ97257.1,
RC ECO:0000313|Proteomes:UP000012015};
RX PubMed=23766407;
RA Shivaji S., Ara S., Bandi S., Singh A., Kumar Pinnaka A.;
RT "Draft Genome Sequence of Arthrobacter gangotriensis Strain Lz1yT, Isolated
RT from a Penguin Rookery Soil Sample Collected in Antarctica, near the Indian
RT Station Dakshin Gangotri.";
RL Genome Announc. 1:e00347-e00313(2013).
CC -!- FUNCTION: Poorly processive, error-prone DNA polymerase involved in
CC untargeted mutagenesis. Copies undamaged DNA at stalled replication
CC forks, which arise in vivo from mismatched or misaligned primer ends.
CC These misaligned primers can be extended by PolIV. Exhibits no 3'-5'
CC exonuclease (proofreading) activity. May be involved in translesional
CC synthesis, in conjunction with the beta clamp from PolIII.
CC {ECO:0000256|ARBA:ARBA00025589}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-Y family.
CC {ECO:0000256|ARBA:ARBA00010945}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EMQ97257.1}.
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DR EMBL; AOCK01000011; EMQ97257.1; -; Genomic_DNA.
DR RefSeq; WP_007272585.1; NZ_AOCK01000011.1.
DR AlphaFoldDB; M7MLS8; -.
DR STRING; 1276920.ADIAG_03424; -.
DR PATRIC; fig|1276920.7.peg.3429; -.
DR eggNOG; COG0389; Bacteria.
DR Proteomes; UP000012015; Unassembled WGS sequence.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR GO; GO:0009432; P:SOS response; IEA:UniProtKB-KW.
DR CDD; cd01700; PolY_Pol_V_umuC; 1.
DR Gene3D; 3.30.70.270; -; 1.
DR Gene3D; 3.40.1170.60; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR017961; DNA_pol_Y-fam_little_finger.
DR InterPro; IPR025188; DUF4113.
DR InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR InterPro; IPR001126; UmuC.
DR PANTHER; PTHR11076; DNA REPAIR POLYMERASE UMUC / TRANSFERASE FAMILY MEMBER; 1.
DR PANTHER; PTHR11076:SF34; PROTEIN UMUC; 1.
DR Pfam; PF13438; DUF4113; 1.
DR Pfam; PF00817; IMS; 1.
DR Pfam; PF11799; IMS_C; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR PROSITE; PS50173; UMUC; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00023199};
KW Nucleotidyltransferase {ECO:0000313|EMBL:EMQ97257.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000012015};
KW SOS mutagenesis {ECO:0000256|ARBA:ARBA00023199};
KW SOS response {ECO:0000256|ARBA:ARBA00023236};
KW Transferase {ECO:0000313|EMBL:EMQ97257.1}.
FT DOMAIN 5..190
FT /note="UmuC"
FT /evidence="ECO:0000259|PROSITE:PS50173"
SQ SEQUENCE 424 AA; 45587 MW; E582C856B6CB64BE CRC64;
MSERIALVDV NNFYVSCERV FDPKLVGRPV VVLSNNDGCV VARSQEAKDL GITTGAPFFK
VKRFVDSHGM IVRSSNYELY GDLSARVMEV LSRFGSWQEV YSIDESFIGI QGSPGQLGEL
ATRIRAAVAR SVGVPVCVGV APTKTLAKFA NHVAKHNAHL EGVCSMDSMD AGLIDHIQSL
VPVTDLWGVG ARTGTRLSGM GISTIADLKS ADVTEIRKKF SVVLQRTVLE LNGTPCIPHN
EERADKQQIM YSRSFSSPVT TIEDMVEVMS IYAQRGASRL MEDGLWATLL TVTAGTSRFA
SGEASFPAVT LKLPAPTQDP ILLTRLAVGA MREIMVQGIS YVRGGVVLSG LQPAPGQETF
GIFAEETTET HVGDVLGAVK AKFGNKSIGL GSGGLAVEPG WSMKREYSSP KFTTDWAQIP
VVKA
//
