ID M7T4E7_EUTLA Unreviewed; 1584 AA.
AC M7T4E7;
DT 29-MAY-2013, integrated into UniProtKB/TrEMBL.
DT 29-MAY-2013, sequence version 1.
DT 27-MAR-2024, entry version 55.
DE RecName: Full=DNA-directed RNA polymerase subunit {ECO:0000256|RuleBase:RU004279};
DE EC=2.7.7.6 {ECO:0000256|RuleBase:RU004279};
GN ORFNames=UCREL1_1205 {ECO:0000313|EMBL:EMR71753.1};
OS Eutypa lata (strain UCR-EL1) (Grapevine dieback disease fungus) (Eutypa
OS armeniacae).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Xylariomycetidae; Xylariales; Diatrypaceae; Eutypa.
OX NCBI_TaxID=1287681 {ECO:0000313|EMBL:EMR71753.1, ECO:0000313|Proteomes:UP000012174};
RN [1] {ECO:0000313|Proteomes:UP000012174}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UCR-EL1 {ECO:0000313|Proteomes:UP000012174};
RX PubMed=23723393; DOI=10.1128/genomeA.00228-13;
RA Blanco-Ulate B., Rolshausen P.E., Cantu D.;
RT "Draft genome sequence of the grapevine dieback fungus Eutypa lata UCR-
RT EL1.";
RL Genome Announc. 1:E0022813-E0022813(2013).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550,
CC ECO:0000256|RuleBase:RU004279};
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000256|ARBA:ARBA00006460, ECO:0000256|RuleBase:RU004279}.
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DR EMBL; KB705590; EMR71753.1; -; Genomic_DNA.
DR RefSeq; XP_007789160.1; XM_007790969.1.
DR STRING; 1287681.M7T4E7; -.
DR KEGG; ela:UCREL1_1205; -.
DR eggNOG; KOG0260; Eukaryota.
DR HOGENOM; CLU_000487_3_0_1; -.
DR OMA; MVQYDRT; -.
DR OrthoDB; 169836at2759; -.
DR Proteomes; UP000012174; Unassembled WGS sequence.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0043229; C:intracellular organelle; IEA:UniProt.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR CDD; cd02584; RNAP_II_Rpb1_C; 1.
DR CDD; cd02733; RNAP_II_RPB1_N; 1.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.150.390; -; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.30.1360.140; -; 1.
DR Gene3D; 6.10.250.2940; -; 1.
DR Gene3D; 6.20.50.80; -; 1.
DR Gene3D; 3.30.1490.180; RNA polymerase ii; 1.
DR Gene3D; 4.10.860.120; RNA polymerase II, clamp domain; 1.
DR Gene3D; 1.10.274.100; RNA polymerase Rpb1, domain 3; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR007075; RNA_pol_Rpb1_6.
DR InterPro; IPR007073; RNA_pol_Rpb1_7.
DR InterPro; IPR038593; RNA_pol_Rpb1_7_sf.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF37; DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR Pfam; PF04992; RNA_pol_Rpb1_6; 1.
DR Pfam; PF04990; RNA_pol_Rpb1_7; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|RuleBase:RU004279}; Membrane {ECO:0000256|SAM:Phobius};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004279};
KW Reference proteome {ECO:0000313|Proteomes:UP000012174};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU004279};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004279};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 1532..1551
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 227..533
FT /note="RNA polymerase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00663"
FT REGION 138..166
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 138..155
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1584 AA; 176537 MW; 0791B0D6221D11AA CRC64;
MIIKEIQFGL LSPEEIKNMS VCRIKYPETM DETRTKPRED GLNDPRLGSV DRQFKCATCG
ESMSECPGHF GHIELAKPVY HPGFIKKIKK VLEVVCHNCS KVLDDRSSPE FAAAVATRDP
KARFQRVWNC CKTKTSCSNE VPKDEDQEYE PGSKPKQKGH GGCGNAQPRV RQTALQLWAT
QSLVSEDNQK TTDKRLITPE MALNILKNIS DEDLADMGLN KDYARPEWMI VTVLPVPPPP
VRPSISMDGT GQGLRNEDDL TYKLGDIIRA NGNVQQSITQ GSPSHICNEY ESLLQYHVAT
LMDNDIAGQP QALQKSGRPV KSIRSRLKGK EGRLRGNLMG KRVDFSARTV ITGDANISLD
EVGVPRSIAR TLTYPETVTP YNISRLHKLV QNGPNEHPGA KYVIRSDGTR IDLRHHKRAG
AISLEYGWKV ERHLQDGDYI IFNRQPSLHK ESMMGHRVKV MPYSTFRLNL SVTSPYNADF
DGDEMNLHVP QTEETRAEIK ELCMVPLNIV SPQRNGPLMG IVQDTLAGVY KMCRRDVFMT
KEAVMNIMLW VPNWDGVIPQ PAILKPRPRW TGKQIVSMII PREISLYTKA KSHPWNPPDD
EGLLVQSGEL IFGLLTKKNV GAAGGGIVHL CYNELGPDGA MAFLNGCQRV INYWLLHNGF
SIGIGDTIPD KSTIEKVQGN INEQKQIVTE LTVEATANTL ECLPGMNIRE SFESKVSKAL
NDARERAGNT AEGSLKDLNN AVVMARSGSK GSAINISQMS ALVGQQVVEG KRIPFGFKYR
TLPHFTKDDY SPEARGFVEN SYLRGLTPTE FYFHAMAGRE GLIDTAVKTA ETGYIQRRLV
KALEDVSAKY DGTVRNSLGD IIQFLYGEDG LDGQIIEKQV MDQWTIRDAD FEGRYRLDVM
AEIPPAALNA LEYAQDISGD IEAQKLLDME FEAIAKDRDF LREFNRGVEK LEDQFQLPFN
IGRLIESSKK LLKLDDAKRS NLTPQDVIPA VKDLLDRMVV VRGPEAVSIE ANESSTLLFK
ALLRSRLSFK RLAVEQRLDK LVFRHVLGEL ESKWQRSFVS PGEMVGVLAA QSIGEPATQM
TLNTFHFTGI AAKNVTLGVP RLKEILNVAS NIKTPSMIVY YDGATATKDH AKKLRSVVEH
TNLRSVTTVA EVYYDPDIQA TTIPEDADMV ESYFLIPDEE HDSIDRQSRW LLRFTLDRQK
LLDKRMTVED VAKRLKEDYA NDLAVIYSDN NAENQVIRVR PMVDDKDGEE KVVEDDVMLK
RLADHLMENL TLRGIKGIEK AFLREGSKIA QDKSGALIQS MGHELCEEWV LDTQGTALRE
VLAVPGVDAT RTVTNDLWQI VDAFGIEAAR GALLNELRRV LAFDGSYVNE RHLALLVDVM
TYRGSIAAVT RHGINRADTG ALMRCSFEET VEILLEAAAT GELDDCMGIS ENVMLGQLAP
MGTGHFEVFL DPKMLDTVIS DNSRMGLMPG MTIKGNQTEG AATPYDTGSP MADSGYGMSL
ASPSMGMGNF SPIVGTGSDT PAGDFPYITL RWLWWRLLAF VSGHWLLAYL ASHRWTLRKF
SSVQPVVALI LSKFSPVPGK PDKS
//
