ID M7ZSS4_TRIUA Unreviewed; 1066 AA.
AC M7ZSS4;
DT 29-MAY-2013, integrated into UniProtKB/TrEMBL.
DT 29-MAY-2013, sequence version 1.
DT 27-MAR-2024, entry version 57.
DE RecName: Full=isoleucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013165};
DE EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
GN ORFNames=TRIUR3_08914 {ECO:0000313|EMBL:EMS62671.1};
OS Triticum urartu (Red wild einkorn) (Crithodium urartu).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4572 {ECO:0000313|EMBL:EMS62671.1};
RN [1] {ECO:0000313|EMBL:EMS62671.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=23535596; DOI=10.1038/nature11997;
RA Ling H.Q., Zhao S., Liu D., Wang J., Sun H., Zhang C., Fan H., Li D.,
RA Dong L., Tao Y., Gao C., Wu H., Li Y., Cui Y., Guo X., Zheng S., Wang B.,
RA Yu K., Liang Q., Yang W., Lou X., Chen J., Feng M., Jian J., Zhang X.,
RA Luo G., Jiang Y., Liu J., Wang Z., Sha Y., Zhang B., Wu H., Tang D.,
RA Shen Q., Xue P., Zou S., Wang X., Liu X., Wang F., Yang Y., An X., Dong Z.,
RA Zhang K., Zhang X., Luo M.C., Dvorak J., Tong Y., Wang J., Yang H., Li Z.,
RA Wang D., Zhang A., Wang J.;
RT "Draft genome of the wheat A-genome progenitor Triticum urartu.";
RL Nature 496:87-90(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-
CC isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666,
CC Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528,
CC ChEBI:CHEBI:456215; EC=6.1.1.5;
CC Evidence={ECO:0000256|ARBA:ARBA00000114};
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DR EMBL; KD079244; EMS62671.1; -; Genomic_DNA.
DR AlphaFoldDB; M7ZSS4; -.
DR STRING; 4572.M7ZSS4; -.
DR eggNOG; KOG0434; Eukaryota.
DR OMA; LLTYWNT; -.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07961; Anticodon_Ia_Ile_ABEc; 1.
DR CDD; cd00818; IleRS_core; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033709; Anticodon_Ile_ABEc.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR023586; Ile-tRNA-ligase_type2.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00392; ileS; 1.
DR PANTHER; PTHR42780:SF1; ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR42780; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF19302; DUF5915; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
PE 4: Predicted;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000313|EMBL:EMS62671.1}; ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917}.
FT DOMAIN 2..584
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 640..712
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
SQ SEQUENCE 1066 AA; 120621 MW; 790CF076FA599A33 CRC64;
MRRFGWDCHG LPVEFEIDKL LGITNRQQVL DLGIGKYNET CRGIVTKYVA EWEAVVTRTG
RWIDFKADYK TMDINFMESV WWVFGQLWDK DLVYKGFKVM PYSTGCKTAL SNFEAGLDYR
TVPDPAVMVS FPVVGDADNA ALVAWTTTPW TLPSNLALCV NANLVYTKVK DKSNGTVYIV
AESRLGQLPV KAKASGKKQA PSKGSNAEAV QGGLDTESYE LLAKIPGSSL VGLKYTPLFD
FFLEFQDTAF RVIADNYVTD DSGTGVVHCA PAFGEDDHRV CLSAGIIEAS GLVVAVDDDG
HFIEKISQFK GRHVKEADKD IINAVKDKGR LVSKGSIEHS YPYCWRSGTP LIYRAVPSWF
IKVEKIRDQL LECNKETYWV PDYVKEKRFH NWLEGARDWA VSRSRFWGTP LPVWISQDGE
EIVVMDSIEK LERLSGVKVN DLHRHHVDDI TIPSSRGPEF GVLKRVEDVF DCWFESGSMP
YAYIHYPFEN RELFEKNFPG NFVAEGLDQT RGWFYTLMVL STALFGKPAF KNLICNGLVL
AEDGKKMSKS KQNYPSPMEV IDEYGADALR LYLVNSPVVR AESLRFKRIG VFGVVKDVFL
PWYNAYRFLV QNAKRLEVEG LTAFSPIDQA SLRKSSNVLD HWIHSATESL VSFVHQEMDA
YRLYTVVPYL VKYIDNLTNI YVRFNRKRLK GRTGEEDCKI SLSTLYHFFT KICGKRQVNQ
SRAFIFAVSL PQLGRDIQVR LLALYYAICT FLQIAAMEMV VVHPDSEFLE DITGKLKEYV
MEEMNVKTVT PCNDPMKYAS LRAEPNFRTF CFLSVLGKRL GKDMGKVSNE VKKMTQEQIL
AFEQSGEISF LGHCLTLDDI KVVRQFKRPV DVSEKEIDAA GDGDVLVILD LRADQSLIEA
GVAREVVNRI QKLRKTAQLE PTDLIDVYYE SVDNSNTLEE ILQSQDQYIR DVLGNSLVPK
AAATSDMVVI CEESHTVHDM SFVIYIARCM PVLAADLLSY ASGNSDHVEA LRVYLLSRSI
SRLKNEFQTG NGKITVKCIE GYPPIDLQLG KHIFLSAGDF YQANRS
//
