ID MAN8_ORYSJ Reviewed; 372 AA.
AC Q0IQJ7; C7J9Y7; Q2QYI9;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 2.
DT 27-MAR-2024, entry version 93.
DE RecName: Full=Mannan endo-1,4-beta-mannosidase 8;
DE EC=3.2.1.78;
DE AltName: Full=Beta-mannanase 8;
DE AltName: Full=Endo-beta-1,4-mannanase 8;
DE AltName: Full=OsMAN8;
GN Name=MAN8; OrderedLocusNames=Os12g0117300, LOC_Os12g02520;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16188032; DOI=10.1186/1741-7007-3-20;
RG The rice chromosomes 11 and 12 sequencing consortia;
RT "The sequence of rice chromosomes 11 and 12, rich in disease resistance
RT genes and recent gene duplications.";
RL BMC Biol. 3:20-20(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP GENE FAMILY, AND TISSUE SPECIFICITY.
RX PubMed=16897088; DOI=10.1007/s10142-006-0034-3;
RA Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F.;
RT "The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar.";
RL Funct. Integr. Genomics 7:1-16(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in
CC mannans, galactomannans and glucomannans.; EC=3.2.1.78;
CC -!- TISSUE SPECIFICITY: Expressed in stems and leaves and seeds.
CC {ECO:0000269|PubMed:16897088}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAH95489.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; DP000011; ABA95648.1; -; Genomic_DNA.
DR EMBL; AP008218; BAH95489.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014968; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; Q0IQJ7; -.
DR SMR; Q0IQJ7; -.
DR STRING; 39947.Q0IQJ7; -.
DR CAZy; GH5; Glycoside Hydrolase Family 5.
DR PaxDb; 39947-Q0IQJ7; -.
DR eggNOG; ENOG502RDYC; Eukaryota.
DR InParanoid; Q0IQJ7; -.
DR Proteomes; UP000000763; Chromosome 12.
DR Proteomes; UP000059680; Chromosome 12.
DR Genevisible; Q0IQJ7; OS.
DR GO; GO:0016985; F:mannan endo-1,4-beta-mannosidase activity; IBA:GO_Central.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR001547; Glyco_hydro_5.
DR InterPro; IPR018087; Glyco_hydro_5_CS.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR045053; MAN-like.
DR PANTHER; PTHR31451; MANNAN ENDO-1,4-BETA-MANNOSIDASE 7; 1.
DR PANTHER; PTHR31451:SF46; MANNAN ENDO-1,4-BETA-MANNOSIDASE 8; 1.
DR Pfam; PF00150; Cellulase; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS00659; GLYCOSYL_HYDROL_F5; 1.
PE 2: Evidence at transcript level;
KW Glycosidase; Hydrolase; Reference proteome.
FT CHAIN 1..372
FT /note="Mannan endo-1,4-beta-mannosidase 8"
FT /id="PRO_0000277489"
FT ACT_SITE 173
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:Q99036"
FT ACT_SITE 293
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:Q99036"
FT BINDING 57
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT BINDING 172
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT BINDING 253
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
FT BINDING 335
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B4XC07"
SQ SEQUENCE 372 AA; 41839 MW; 4FB08926901C47EE CRC64;
MVECSGTQLW ASGRPFIIHG FNTYWLMSFA ADQATRPRVT AAIAEAAEAG LNVCRTWAFS
DGGYRALQTV PFHYDEDVFQ ALDFVVSEAK RHNMRLILSL CNNWEDYGGK AQYVRWGKEA
GLDLTSEDDF FSDPTIKSYY KAFVEAVVTR INTVTNETYK DDPTILAWEL INEPRCPSDP
SGDTLQAWIE EMASYVKSID PVHLLEIGIE GFYGLSTPEL LPVNPDEYSG HAGTDFIRNH
QAPGIDLASI HVYSDTWLPH SIKENHLQFV DKWMQQHIHD AANLLGMPIV VGEFGVSVKD
GKFGNEFRED FMKTVYRIFL SSWKEGVIGG GCLLWQLFPE GAEHMDDGYA VIFAKSPSTL
SLLANHLRCL EC
//
