ID MINE_ECOLI Reviewed; 88 AA.
AC P0A734; P18198;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2005, sequence version 1.
DT 24-JAN-2024, entry version 126.
DE RecName: Full=Cell division topological specificity factor;
GN Name=minE; OrderedLocusNames=b1174, JW1163;
OS Escherichia coli (strain K12).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2645057; DOI=10.1016/0092-8674(89)90586-2;
RA de Boer P.A.J., Crossley R.E., Rothfield L.I.;
RT "A division inhibitor and a topological specificity factor coded for by the
RT minicell locus determine proper placement of the division septum in E.
RT coli.";
RL Cell 56:641-649(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP CLEAVAGE OF INITIATOR METHIONINE.
RC STRAIN=K12 / CAG12184, and K12 / PAL421Tr;
RX PubMed=26017780; DOI=10.1002/pmic.201500027;
RA Bienvenut W.V., Giglione C., Meinnel T.;
RT "Proteome-wide analysis of the amino terminal status of Escherichia coli
RT proteins at the steady-state and upon deformylation inhibition.";
RL Proteomics 15:2503-2518(2015).
RN [6]
RP FUNCTION IN SUBCELLULAR LOCATION AT CELL POLES, AND DISRUPTION PHENOTYPE.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=22380631; DOI=10.1111/j.1365-2958.2012.08021.x;
RA Li G., Young K.D.;
RT "Isolation and identification of new inner membrane-associated proteins
RT that localize to cell poles in Escherichia coli.";
RL Mol. Microbiol. 84:276-295(2012).
CC -!- FUNCTION: Prevents the cell division inhibition by proteins MinC and
CC MinD at internal division sites while permitting inhibition at polar
CC sites. This ensures cell division at the proper site by restricting the
CC formation of a division septum at the midpoint of the long axis of the
CC cell. {ECO:0000269|PubMed:22380631}.
CC -!- INTERACTION:
CC P0A734; P0AEZ3: minD; NbExp=4; IntAct=EBI-1118020, EBI-554545;
CC P0A734; P0A734: minE; NbExp=4; IntAct=EBI-1118020, EBI-1118020;
CC -!- DISRUPTION PHENOTYPE: In a minCDE operon disruption (minC-minD-minE),
CC cells divide not only at midpoint but also at their poles, yielding
CC small minicells and long rods. Loss of polar localization of several
CC polar-localized proteins including GroEL-GroES, TnaA and YqjD.
CC {ECO:0000269|PubMed:22380631}.
CC -!- SIMILARITY: Belongs to the MinE family. {ECO:0000305}.
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DR EMBL; J03153; AAB59063.1; -; Genomic_DNA.
DR EMBL; U00096; AAC74258.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA36008.1; -; Genomic_DNA.
DR PIR; C31877; CEECTF.
DR RefSeq; NP_415692.1; NC_000913.3.
DR RefSeq; WP_001185665.1; NZ_STEB01000023.1.
DR PDB; 1EV0; NMR; -; A/B=31-88.
DR PDB; 3R9I; X-ray; 2.60 A; E/F/G/H=12-31.
DR PDB; 3R9J; X-ray; 4.30 A; C/D=12-88.
DR PDBsum; 1EV0; -.
DR PDBsum; 3R9I; -.
DR PDBsum; 3R9J; -.
DR AlphaFoldDB; P0A734; -.
DR SMR; P0A734; -.
DR BioGRID; 4262867; 340.
DR BioGRID; 850107; 4.
DR DIP; DIP-35912N; -.
DR IntAct; P0A734; 19.
DR STRING; 511145.b1174; -.
DR jPOST; P0A734; -.
DR PaxDb; 511145-b1174; -.
DR EnsemblBacteria; AAC74258; AAC74258; b1174.
DR GeneID; 83576939; -.
DR GeneID; 945740; -.
DR KEGG; ecj:JW1163; -.
DR KEGG; eco:b1174; -.
DR PATRIC; fig|1411691.4.peg.1114; -.
DR EchoBASE; EB0593; -.
DR eggNOG; COG0851; Bacteria.
DR HOGENOM; CLU_137929_2_2_6; -.
DR InParanoid; P0A734; -.
DR OMA; QIIVARE; -.
DR OrthoDB; 9802655at2; -.
DR PhylomeDB; P0A734; -.
DR BioCyc; EcoCyc:EG10598-MONOMER; -.
DR EvolutionaryTrace; P0A734; -.
DR PRO; PR:P0A734; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0042802; F:identical protein binding; IDA:EcoCyc.
DR GO; GO:0000918; P:division septum site selection; IDA:EcoCyc.
DR GO; GO:0051302; P:regulation of cell division; IDA:EcoCyc.
DR GO; GO:0032955; P:regulation of division septum assembly; IDA:EcoCyc.
DR Gene3D; 3.30.1070.10; Cell division topological specificity factor MinE; 1.
DR HAMAP; MF_00262; MinE; 1.
DR InterPro; IPR005527; MinE.
DR InterPro; IPR036707; MinE_sf.
DR NCBIfam; TIGR01215; minE; 1.
DR Pfam; PF03776; MinE; 1.
DR SUPFAM; SSF55229; Cell division protein MinE topological specificity domain; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell cycle; Cell division; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:26017780"
FT CHAIN 2..88
FT /note="Cell division topological specificity factor"
FT /id="PRO_0000205874"
FT HELIX 14..25
FT /evidence="ECO:0007829|PDB:3R9I"
FT HELIX 32..34
FT /evidence="ECO:0007829|PDB:1EV0"
FT HELIX 39..53
FT /evidence="ECO:0007829|PDB:1EV0"
FT HELIX 58..60
FT /evidence="ECO:0007829|PDB:1EV0"
FT STRAND 61..68
FT /evidence="ECO:0007829|PDB:1EV0"
FT TURN 69..71
FT /evidence="ECO:0007829|PDB:1EV0"
FT STRAND 72..81
FT /evidence="ECO:0007829|PDB:1EV0"
SQ SEQUENCE 88 AA; 10235 MW; 04DDFB4EA24F96C1 CRC64;
MALLDFFLSR KKNTANIAKE RLQIIVAERR RSDAEPHYLP QLRKDILEVI CKYVQIDPEM
VTVQLEQKDG DISILELNVT LPEAEELK
//
