ID MORC1_MOUSE Reviewed; 950 AA.
AC Q9WVL5;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 24-JAN-2024, entry version 141.
DE RecName: Full=MORC family CW-type zinc finger protein 1;
DE AltName: Full=Protein microrchidia;
GN Name=Morc1 {ECO:0000312|MGI:MGI:1316740};
GN Synonyms=Morc {ECO:0000312|EMBL:AAD43003.1};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1] {ECO:0000312|EMBL:AAD43003.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=10369865; DOI=10.1093/hmg/8.7.1201;
RA Inoue N., Hess K.D., Moreadith R.W., Richardson L.L., Handel M.A.,
RA Watson M.L., Zinn A.R.;
RT "New gene family defined by MORC, a nuclear protein required for mouse
RT spermatogenesis.";
RL Hum. Mol. Genet. 8:1201-1207(1999).
RN [2] {ECO:0000312|EMBL:AAH30893.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N {ECO:0000312|EMBL:AAH30893.1};
RC TISSUE=Mammary gland {ECO:0000312|EMBL:AAH30893.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP DISRUPTION PHENOTYPE.
RX PubMed=26275923; DOI=10.1016/j.bbr.2015.08.005;
RA Schmidt M., Brandwein C., Luoni A., Sandrini P., Calzoni T., Deuschle M.,
RA Cirulli F., Riva M.A., Gass P.;
RT "Morc1 knockout evokes a depression-like phenotype in mice.";
RL Behav. Brain Res. 296:7-14(2016).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=25503965; DOI=10.1038/ncomms6795;
RA Pastor W.A., Stroud H., Nee K., Liu W., Pezic D., Manakov S., Lee S.A.,
RA Moissiard G., Zamudio N., Bourc'his D., Aravin A.A., Clark A.T.,
RA Jacobsen S.E.;
RT "MORC1 represses transposable elements in the mouse male germline.";
RL Nat. Commun. 5:5795-5795(2014).
CC -!- FUNCTION: Required for spermatogenesis (PubMed:10369865,
CC PubMed:25503965). Essential for de novo DNA methylation and silencing
CC of transposable elements in the male embryonic germ cells
CC (PubMed:25503965). Not required for piRNA biosynthesis
CC (PubMed:25503965). {ECO:0000269|PubMed:10369865,
CC ECO:0000269|PubMed:25503965}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10369865,
CC ECO:0000269|PubMed:25503965}.
CC -!- TISSUE SPECIFICITY: Expressed at very low level in male germ cells.
CC {ECO:0000269|PubMed:10369865}.
CC -!- DEVELOPMENTAL STAGE: Detected in embryonic testis at embryonic days
CC 14.5 dpc and peaks at 16.5 dpc. {ECO:0000269|PubMed:25503965}.
CC -!- DISRUPTION PHENOTYPE: Mice show progressive loss of spermatogonia and
CC male infertility (PubMed:10369865, PubMed:25503965). Spermatogenesis is
CC blocked early in meiosis I (PubMed:10369865, PubMed:25503965). Male
CC germ cells show derepression of transposable elements (TEs) and DNA
CC hypomethylation of TEs (PubMed:25503965). Mice display increased
CC depressive-like behavior whereas no behavioral abnormalities regarding
CC locomotor activity or anxiety-like behavior are detectable and BDNF
CC levels in the hippocampus are up-regulated (PubMed:26275923).
CC {ECO:0000269|PubMed:10369865, ECO:0000269|PubMed:25503965,
CC ECO:0000269|PubMed:26275923}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF084945; AAD43003.1; -; mRNA.
DR EMBL; BC030893; AAH30893.1; -; mRNA.
DR CCDS; CCDS37351.1; -.
DR RefSeq; NP_034946.1; NM_010816.1.
DR AlphaFoldDB; Q9WVL5; -.
DR SMR; Q9WVL5; -.
DR STRING; 10090.ENSMUSP00000023330; -.
DR iPTMnet; Q9WVL5; -.
DR PhosphoSitePlus; Q9WVL5; -.
DR PaxDb; 10090-ENSMUSP00000023330; -.
DR PeptideAtlas; Q9WVL5; -.
DR ProteomicsDB; 252592; -.
DR Antibodypedia; 32406; 131 antibodies from 19 providers.
DR DNASU; 17450; -.
DR Ensembl; ENSMUST00000023330.8; ENSMUSP00000023330.7; ENSMUSG00000022652.8.
DR GeneID; 17450; -.
DR KEGG; mmu:17450; -.
DR UCSC; uc007zjp.1; mouse.
DR AGR; MGI:1316740; -.
DR CTD; 27136; -.
DR MGI; MGI:1316740; Morc1.
DR VEuPathDB; HostDB:ENSMUSG00000022652; -.
DR eggNOG; KOG1845; Eukaryota.
DR GeneTree; ENSGT00940000153998; -.
DR HOGENOM; CLU_011516_0_1_1; -.
DR InParanoid; Q9WVL5; -.
DR OMA; LDIWICA; -.
DR OrthoDB; 933627at2759; -.
DR PhylomeDB; Q9WVL5; -.
DR TreeFam; TF329118; -.
DR BioGRID-ORCS; 17450; 2 hits in 75 CRISPR screens.
DR ChiTaRS; Morc1; mouse.
DR PRO; PR:Q9WVL5; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q9WVL5; Protein.
DR Bgee; ENSMUSG00000022652; Expressed in otic capsule and 35 other cell types or tissues.
DR Genevisible; Q9WVL5; MM.
DR GO; GO:0001673; C:male germ cell nucleus; IDA:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0001662; P:behavioral fear response; IMP:MGI.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0044727; P:epigenetic programing of male pronucleus; IMP:MGI.
DR GO; GO:0040029; P:epigenetic regulation of gene expression; IMP:MGI.
DR GO; GO:2000143; P:negative regulation of DNA-templated transcription initiation; IMP:MGI.
DR GO; GO:0010629; P:negative regulation of gene expression; IMP:MGI.
DR GO; GO:0031047; P:regulatory ncRNA-mediated gene silencing; IEA:UniProtKB-KW.
DR GO; GO:0032197; P:retrotransposition; IMP:MGI.
DR GO; GO:0141005; P:retrotransposon silencing by heterochromatin formation; IMP:MGI.
DR GO; GO:0007283; P:spermatogenesis; IMP:MGI.
DR CDD; cd16931; HATPase_MORC-like; 1.
DR Gene3D; 3.30.40.100; -; 1.
DR Gene3D; 3.30.565.10; Histidine kinase-like ATPase, C-terminal domain; 1.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR041006; Morc_S5.
DR InterPro; IPR011124; Znf_CW.
DR PANTHER; PTHR23337:SF6; MORC FAMILY CW-TYPE ZINC FINGER PROTEIN 1; 1.
DR PANTHER; PTHR23337; ZINC FINGER CW-TYPE COILED-COIL DOMAIN PROTEIN 1; 1.
DR Pfam; PF13589; HATPase_c_3; 1.
DR Pfam; PF17942; Morc6_S5; 1.
DR Pfam; PF07496; zf-CW; 1.
DR SUPFAM; SSF55874; ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase; 1.
DR PROSITE; PS51050; ZF_CW; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Developmental protein; Differentiation; Metal-binding;
KW Nucleus; Reference proteome; RNA-mediated gene silencing; Spermatogenesis;
KW Zinc; Zinc-finger.
FT CHAIN 1..950
FT /note="MORC family CW-type zinc finger protein 1"
FT /id="PRO_0000248242"
FT ZN_FING 465..530
FT /note="CW-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00454"
FT REGION 532..552
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 679..700
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 281..342
FT /evidence="ECO:0000255"
FT COILED 885..916
FT /evidence="ECO:0000255"
FT BINDING 485
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00454"
FT BINDING 488
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00454"
FT BINDING 511
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00454"
FT BINDING 522
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00454"
SQ SEQUENCE 950 AA; 108310 MW; 0DE2D3D30DB18F43 CRC64;
MDKYALLQRA KLHLDFIHAN STTHSFLFGA LAELLDNARD AGAVRLDVFS VDNETLQGGF
MLCFLDDGCG MSPDEASDVI YFGTSKKRLS TLKFIGQYGN GLKSGSMRIG KDCILFTKKE
ETMTCLFFSQ TFCEKEGLTE VVVPIPSWLT RTRESITDDP QKFFTELSII FKYSPFKTEA
ELMQQFDMIY GRCGTLLIIY NLKLLLSGEP ELDVTTDKED ILMAEAPEEI PERRSFRAYT
AVLYFEPRMK IFIQAKRVQT KHLCYSLYKP RKYQYTTSSF KGKFKTEVQK AEEAVKRAEL
LFKEVQAKVN QPDRIALSST QDALQKALQD VDTKHKSLRQ KQRALRKART LSLFFGVNTE
DQHQAGMFIY SNNRLIKMYE KVGPQLKMKS LLGAGIIGIV NIPLETMEPS HNKQEFLNVQ
EYNHLLKVMG QYLIQYCKDI GISNRNLTLF WDEFKYQHSK DTDSSLESLQ WRRRQAMGIP
FILQCDLCLK WRVLPSSSNY QEKGLPDLWI CASNPNNLEN SCNQIERLPS IPLGTVNRRP
PSKDERERQL QESVQRYQDK LVEAQPQKSQ LIVTSKIPEF KSSCLSSALK EKSKLGRIQP
SGADLTQGSP SSVKLSFMQR SQKRSTEDTH SDVEFICMTK IPKKSVKKTV KYLQPGHAPA
LLENLKLEDT AQVSSREIKK QQSESLVQAG KASTDVASSR DPTVTMVWDQ SSTKVSLKQE
EEEEVPLIKP DKQELCDDTP VVKGNSSALH WKSLPGVQME DLSPRSGHKI NSVSGDCQLP
ASPMPSQSMS VEETARKLLS NLREILLYFV PEFQLSSEFE CTSVEELITN PELERCPENI
NEKLKTCFNQ IQNIYMAQYE KRLKRKMQSI VYEANRRGLL NQVFLGQCEL KRKRTEEKLS
DLRAKLALLL QKLQLGGPAG DPQQIDAYLE DLLKEDRLPT ALHEKSPESA
//
