ID MT1_HOMAM Reviewed; 58 AA.
AC P29499;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 14-DEC-2022, entry version 82.
DE RecName: Full=Metallothionein-1;
DE AltName: Full=CuMT-1;
OS Homarus americanus (American lobster).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Multicrustacea;
OC Malacostraca; Eumalacostraca; Eucarida; Decapoda; Pleocyemata; Astacidea;
OC Nephropoidea; Nephropidae; Homarus.
OX NCBI_TaxID=6706;
RN [1]
RP PROTEIN SEQUENCE.
RC TISSUE=Hepatopancreas;
RX PubMed=2709004; DOI=10.1016/0162-0134(89)84018-8;
RA Brouwer M., Winge D.R., Gray W.R.;
RT "Structural and functional diversity of copper-metallothioneins from the
RT American lobster Homarus americanus.";
RL J. Inorg. Biochem. 35:289-303(1989).
RN [2]
RP STRUCTURE BY NMR.
RX PubMed=8043573; DOI=10.1021/bi00196a002;
RA Zhu Z., Derose E.F., Mullen G.P., Petering D.H., Shaw C.F. III;
RT "Sequential proton resonance assignments and metal cluster topology of
RT lobster metallothionein-1.";
RL Biochemistry 33:8858-8865(1994).
RN [3] {ECO:0007744|PDB:1J5L, ECO:0007744|PDB:1J5M}
RP STRUCTURE BY NMR IN COMPLEX WITH CADMIUM IONS.
RX PubMed=12203008; DOI=10.1007/s00775-002-0345-3;
RA Munoz A., Foersterling F.H., Shaw C.F. III, Petering D.H.;
RT "Structure of the (113)Cd(3)beta domains from Homarus americanus
RT metallothionein-1: hydrogen bonding and solvent accessibility of sulfur
RT atoms.";
RL J. Biol. Inorg. Chem. 7:713-724(2002).
CC -!- FUNCTION: Metallothioneins have a high content of cysteine residues
CC that bind various heavy metals. The different forms of lobster
CC metallothioneins may have different biological functions. Class I MTS
CC in marine crustacea are involved in the sequestration of elevated
CC levels of heavy-metal ions. Binds 6 metal ions. Known to bind cadmium.
CC -!- SIMILARITY: Belongs to the metallothionein superfamily. Type 3 family.
CC {ECO:0000305}.
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DR PIR; A37039; A37039.
DR PDB; 1J5L; NMR; -; A=29-56.
DR PDB; 1J5M; NMR; -; A=1-28.
DR PDBsum; 1J5L; -.
DR PDBsum; 1J5M; -.
DR BMRB; P29499; -.
DR SMR; P29499; -.
DR EvolutionaryTrace; P29499; -.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR InterPro; IPR002045; Metalthion_crustacean.
DR InterPro; IPR017854; Metalthion_dom_sf.
DR PRINTS; PR00858; MTCRUSTACEAN.
DR SUPFAM; SSF57868; Metallothionein; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Cadmium; Copper; Direct protein sequencing; Metal-binding;
KW Metal-thiolate cluster.
FT CHAIN 1..58
FT /note="Metallothionein-1"
FT /id="PRO_0000197342"
FT REGION 1..28
FT /note="Beta"
FT REGION 29..58
FT /note="Alpha"
FT BINDING 4
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:12203008,
FT ECO:0007744|PDB:1J5M"
FT BINDING 5
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:12203008,
FT ECO:0007744|PDB:1J5M"
FT BINDING 5
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:12203008,
FT ECO:0007744|PDB:1J5M"
FT BINDING 9
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:12203008,
FT ECO:0007744|PDB:1J5M"
FT BINDING 11
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="3"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:12203008,
FT ECO:0007744|PDB:1J5M"
FT BINDING 16
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:12203008,
FT ECO:0007744|PDB:1J5M"
FT BINDING 16
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="3"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:12203008,
FT ECO:0007744|PDB:1J5M"
FT BINDING 20
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:12203008,
FT ECO:0007744|PDB:1J5M"
FT BINDING 22
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:12203008,
FT ECO:0007744|PDB:1J5M"
FT BINDING 25
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:12203008,
FT ECO:0007744|PDB:1J5M"
FT BINDING 25
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="3"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:12203008,
FT ECO:0007744|PDB:1J5M"
FT BINDING 27
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="3"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:12203008,
FT ECO:0007744|PDB:1J5M"
FT BINDING 30
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="4"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:12203008,
FT ECO:0007744|PDB:1J5L"
FT BINDING 33
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="4"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:12203008,
FT ECO:0007744|PDB:1J5L"
FT BINDING 33
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="5"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:12203008,
FT ECO:0007744|PDB:1J5L"
FT BINDING 37
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="5"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:12203008,
FT ECO:0007744|PDB:1J5L"
FT BINDING 39
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="6"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:12203008,
FT ECO:0007744|PDB:1J5L"
FT BINDING 45
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="6"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:12203008,
FT ECO:0007744|PDB:1J5L"
FT BINDING 49
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="4"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:12203008,
FT ECO:0007744|PDB:1J5L"
FT BINDING 49
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="6"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:12203008,
FT ECO:0007744|PDB:1J5L"
FT BINDING 53
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="4"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:12203008,
FT ECO:0007744|PDB:1J5L"
FT BINDING 55
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="5"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:12203008,
FT ECO:0007744|PDB:1J5L"
FT BINDING 56
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="5"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:12203008,
FT ECO:0007744|PDB:1J5L"
FT BINDING 56
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="6"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:12203008,
FT ECO:0007744|PDB:1J5L"
FT STRAND 6..8
FT /evidence="ECO:0007829|PDB:1J5M"
FT STRAND 11..14
FT /evidence="ECO:0007829|PDB:1J5M"
FT HELIX 31..33
FT /evidence="ECO:0007829|PDB:1J5L"
FT HELIX 45..48
FT /evidence="ECO:0007829|PDB:1J5L"
SQ SEQUENCE 58 AA; 5977 MW; 176ABAAF60A32F96 CRC64;
PGPCCKDKCE CAEGGCKTGC KCTSCRCAPC EKCTSGCKCP SKDECAKTCS KPCSCCXX
//
