ID MTRB_METTH Reviewed; 100 AA.
AC O27228;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 27-MAR-2024, entry version 124.
DE RecName: Full=Tetrahydromethanopterin S-methyltransferase subunit B;
DE EC=2.1.1.86;
DE AltName: Full=N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit B;
GN Name=mtrB; OrderedLocusNames=MTH_1160;
OS Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX NCBI_TaxID=187420;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA Reeve J.N.;
RT "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT functional analysis and comparative genomics.";
RL J. Bacteriol. 179:7135-7155(1997).
CC -!- FUNCTION: Part of a complex that catalyzes the formation of methyl-
CC coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-
CC tetrahydromethanopterin. This is an energy-conserving, sodium-ion
CC translocating step. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2
CC Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2
CC Na(+)(out); Xref=Rhea:RHEA:53492, ChEBI:CHEBI:29101,
CC ChEBI:CHEBI:58103, ChEBI:CHEBI:58116, ChEBI:CHEBI:58286,
CC ChEBI:CHEBI:58319; EC=2.1.1.86;
CC -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); methyl-
CC coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step
CC 2/2.
CC -!- SUBUNIT: The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD,
CC MtrE, MtrF, MtrG and MtrH. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the MtrB family. {ECO:0000305}.
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DR EMBL; AE000666; AAB85649.1; -; Genomic_DNA.
DR PIR; F69021; F69021.
DR RefSeq; WP_010876784.1; NC_000916.1.
DR AlphaFoldDB; O27228; -.
DR STRING; 187420.MTH_1160; -.
DR PaxDb; 187420-MTH_1160; -.
DR EnsemblBacteria; AAB85649; AAB85649; MTH_1160.
DR GeneID; 77401689; -.
DR KEGG; mth:MTH_1160; -.
DR PATRIC; fig|187420.15.peg.1137; -.
DR HOGENOM; CLU_171544_0_0_2; -.
DR InParanoid; O27228; -.
DR UniPathway; UPA00640; UER00698.
DR Proteomes; UP000005223; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030269; F:tetrahydromethanopterin S-methyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01094; MtrB; 1.
DR InterPro; IPR008690; MtrB_MeTrfase.
DR NCBIfam; TIGR04166; methano_MtrB; 1.
DR Pfam; PF05440; MtrB; 1.
DR PIRSF; PIRSF005518; MtrB; 1.
PE 3: Inferred from homology;
KW Cell membrane; Membrane; Methanogenesis; Methyltransferase;
KW One-carbon metabolism; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..100
FT /note="Tetrahydromethanopterin S-methyltransferase subunit
FT B"
FT /id="PRO_0000147518"
FT TRANSMEM 80..100
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 100 AA; 10828 MW; 141FBC3813FFDC3E CRC64;
MEMLPLVKIA PEYNLTLDPS TGMIGAALGR EVIILSMDEI NEQIAELEAT ADDLINSLDP
TTTPLDSYPG REGVYLTAGK LTNMVYGFIL GLIILFALLL
//
