ID N1JNP8_BLUG1 Unreviewed; 1068 AA.
AC N1JNP8;
DT 26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2013, sequence version 1.
DT 24-JAN-2024, entry version 45.
DE RecName: Full=DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00012418};
DE EC=2.7.7.6 {ECO:0000256|ARBA:ARBA00012418};
GN ORFNames=BGHDH14_bgh05376 {ECO:0000313|EMBL:CCU82236.1};
OS Blumeria graminis f. sp. hordei (strain DH14) (Barley powdery mildew)
OS (Oidium monilioides f. sp. hordei).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC Erysiphales; Erysiphaceae; Blumeria; Blumeria hordei.
OX NCBI_TaxID=546991 {ECO:0000313|EMBL:CCU82236.1, ECO:0000313|Proteomes:UP000015441};
RN [1] {ECO:0000313|EMBL:CCU82236.1, ECO:0000313|Proteomes:UP000015441}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DH14 {ECO:0000313|EMBL:CCU82236.1};
RX PubMed=21148392; DOI=10.1126/science.1194573;
RA Spanu P.D., Abbott J.C., Amselem J., Burgis T.A., Soanes D.M., Stueber K.,
RA Ver Loren van Themaat E., Brown J.K.M., Butcher S.A., Gurr S.J.,
RA Lebrun M.-H., Ridout C.J., Schulze-Lefert P., Talbot N.J., Ahmadinejad N.,
RA Ametz C., Barton G.R., Benjdia M., Bidzinski P., Bindschedler L.V.,
RA Both M., Brewer M.T., Cadle-Davidson L., Cadle-Davidson M.M., Collemare J.,
RA Cramer R., Frenkel O., Godfrey D., Harriman J., Hoede C., King B.C.,
RA Klages S., Kleemann J., Knoll D., Koti P.S., Kreplak J., Lopez-Ruiz F.J.,
RA Lu X., Maekawa T., Mahanil S., Micali C., Milgroom M.G., Montana G.,
RA Noir S., O'Connell R.J., Oberhaensli S., Parlange F., Pedersen C.,
RA Quesneville H., Reinhardt R., Rott M., Sacristan S., Schmidt S.M.,
RA Schoen M., Skamnioti P., Sommer H., Stephens A., Takahara H.,
RA Thordal-Christensen H., Vigouroux M., Wessling R., Wicker T., Panstruga R.;
RT "Genome expansion and gene loss in powdery mildew fungi reveal tradeoffs in
RT extreme parasitism.";
RL Science 330:1543-1546(2010).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCU82236.1}.
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DR EMBL; CAUH01006045; CCU82236.1; -; Genomic_DNA.
DR AlphaFoldDB; N1JNP8; -.
DR STRING; 546991.N1JNP8; -.
DR EnsemblFungi; BLGH_06610-mRNA-1; BLGH_06610-mRNA-1; BLGH_06610.
DR eggNOG; KOG0262; Eukaryota.
DR HOGENOM; CLU_000487_2_0_1; -.
DR InParanoid; N1JNP8; -.
DR OrthoDB; 169836at2759; -.
DR Proteomes; UP000015441; Unassembled WGS sequence.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR CDD; cd02735; RNAP_I_Rpa1_C; 1.
DR CDD; cd01435; RNAP_I_RPA1_N; 1.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.150.390; -; 1.
DR Gene3D; 1.10.357.120; -; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.30.70.2850; -; 1.
DR Gene3D; 1.10.274.100; RNA polymerase Rpb1, domain 3; 1.
DR InterPro; IPR047107; DNA-dir_RNA_pol1_lsu_C.
DR InterPro; IPR015699; DNA-dir_RNA_pol1_lsu_N.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF11; DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
PE 4: Predicted;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000313|EMBL:CCU82236.1};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000015441};
KW Transcription {ECO:0000256|ARBA:ARBA00023163};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 1..36
FT /note="RNA polymerase alpha subunit"
FT /evidence="ECO:0000259|Pfam:PF00623"
FT DOMAIN 42..218
FT /note="RNA polymerase Rpb1"
FT /evidence="ECO:0000259|Pfam:PF04983"
FT DOMAIN 301..368
FT /note="RNA polymerase Rpb1"
FT /evidence="ECO:0000259|Pfam:PF05000"
FT DOMAIN 375..1009
FT /note="RNA polymerase Rpb1"
FT /evidence="ECO:0000259|Pfam:PF04998"
FT REGION 717..740
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 754..838
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 767..801
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 809..827
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1068 AA; 117881 MW; BB5FDB83F3123389 CRC64;
MHYANCNTYN ADFDGDEMNM HFPQNEIARA EALQIADTDH QYLVATSGKP LRGLIQDHIS
VSVWMCNRDT FFDKATYQQL IYNCLRPENG HIVGDRIETV PPAFIKPRLL WSGKQVITTI
LKNITPVDCA PLALTSKSQI PSERWGSNSE EGVVHFVGGE FITGILDKSQ IGPSGGGLVH
SIHEVYGPTV AGKLLSILGR LLTKFLHIRG FTCGMDDLRL TPQGEQIRKE KIKEAQNIGL
DVAARYVTMA DQNPSNTDPE LLRRLQDVTH DDSKQAGLDM MMNKRSGELS SSITLACLPS
GLVKQFPRNQ MQNMTVSGAK GSAVNANLIS CNLGQQVLEG RRVPIMVSGK SLPCFKPYET
HVRAGGYIAN RFLTGIRPQE YYFHAMAGRE GLIDTAVKTS RSGYLQRCLI KGMEGLKVEY
DTSVRDSDGS MIQFIYGEDG LDVTKQKHLT DFKFLLQNLV SELSQLNYGE PRYDGIFDKK
ENILKRMKRA IRHAKAGDHN GPDPVLSEFN PGRYAYATSE KFFESLTNYL KLNPDGVVKD
KQHPNGVGIR KALEPVLVAK YLRSVVEPGE AVGIVAGQSV GEPSTQMTLN TFHLAGHSAK
NVTLGIPRLR EIVMTASNKI STPSMTLRLN PELSHSDGEK FAKAISILPL SEVTKEATVK
ERIGKGNTFP QAKIYDIHIQ LFPSKDYMEH HAISIFDVLN TFEMKFTPKL QTLTRKALGK
RKESNKTATA AMPEIGQSIG VIEEGTAAID RESMHQAGEE DDDDEDATNE KQKHNRTEAV
SYAENDEDDD RVQRQAQKEG ETIEEEEEGA GALGTHDSSD GEEDETAPTK ISSASIKEHE
ADVMKRCSDI SRFNFDEENG EWCDMALEYD ADVPKILMLS IVEKALQESL IQQIPGLSAC
NFEPDVKVKD VESGKFIMSS VVHTTGVNLK AMQAFPHIIN PNTISTNDIA AVLAHYGVEA
CRAAIIQELL AVFGGHSISV DPRHLNLIAD FMTRGGGFTP FNRNGMKGNV SPFMKMSFET
TVGFLVEAVC DGDWDDLSNP SSRIVMGKMS KVGTGAFDVF TRVPTSKA
//
