ID N1NY63_YEASC Unreviewed; 4910 AA.
AC N1NY63;
DT 26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2013, sequence version 1.
DT 27-MAR-2024, entry version 40.
DE RecName: Full=Midasin {ECO:0000256|ARBA:ARBA00017143, ECO:0000256|PIRNR:PIRNR010340};
GN ORFNames=CENPK1137D_494 {ECO:0000313|EMBL:EIW08724.1};
OS Saccharomyces cerevisiae (strain CEN.PK113-7D) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=889517 {ECO:0000313|EMBL:EIW08724.1};
RN [1] {ECO:0000313|EMBL:EIW08724.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CEN.PK113-7D {ECO:0000313|EMBL:EIW08724.1};
RA Nijkamp J.F., van den Broek M.A., Datema E., de Kok S., Bosman L.,
RA Luttink M.A., Daran-Lapujade P., Vongsangnak W., Nielsen J., Heijne W.H.M.,
RA Klaassen P., Platt D., Paddon C.J., Koetter P., van Ham R.C.,
RA Reinders M.J.T., Pronk J.T., de Ridder D., Daran J.-M.;
RT "De novo sequencing, assembly and analysis of the genome of the laboratory
RT strain Saccharomyces cerevisiae CEN.PK113-7D, a model for modern industrial
RT biotechnology.";
RL Submitted (MAR-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Nuclear chaperone required for maturation and nuclear export
CC of pre-60S ribosome subunits. {ECO:0000256|PIRNR:PIRNR010340}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000256|ARBA:ARBA00004604}. Nucleus, nucleoplasm
CC {ECO:0000256|ARBA:ARBA00004642}.
CC -!- SIMILARITY: Belongs to the midasin family.
CC {ECO:0000256|ARBA:ARBA00007188, ECO:0000256|PIRNR:PIRNR010340}.
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DR EMBL; CM001533; EIW08724.1; -; Genomic_DNA.
DR HOGENOM; CLU_000050_0_2_1; -.
DR Proteomes; UP000013192; Chromosome XII.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR CDD; cd00009; AAA; 2.
DR CDD; cd01460; vWA_midasin; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 6.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR040848; AAA_lid_7.
DR InterPro; IPR011704; ATPase_dyneun-rel_AAA.
DR InterPro; IPR048617; MDN1_AAA_lid_4.
DR InterPro; IPR012099; Midasin.
DR InterPro; IPR041190; Midasin_AAA_lid_5.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR InterPro; IPR002035; VWF_A.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR48103:SF2; MIDASIN; 1.
DR PANTHER; PTHR48103; MIDASIN-RELATED; 1.
DR Pfam; PF07728; AAA_5; 8.
DR Pfam; PF17865; AAA_lid_5; 1.
DR Pfam; PF17867; AAA_lid_7; 3.
DR Pfam; PF21108; MDN1_4th; 1.
DR PIRSF; PIRSF010340; Midasin; 1.
DR SMART; SM00382; AAA; 6.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 6.
DR SUPFAM; SSF53300; vWA-like; 1.
DR PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR PROSITE; PS50234; VWFA; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PIRNR:PIRNR010340};
KW Chaperone {ECO:0000256|ARBA:ARBA00023186, ECO:0000256|PIRNR:PIRNR010340};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|PIRNR:PIRNR010340};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|PIRNR:PIRNR010340}.
FT DOMAIN 4704..4899
FT /note="VWFA"
FT /evidence="ECO:0000259|PROSITE:PS50234"
FT REGION 4045..4600
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 750..780
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 4099..4152
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4153..4205
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4206..4248
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4255..4276
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4277..4302
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4303..4351
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4352..4382
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4395..4430
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4431..4449
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4456..4497
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4498..4517
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4518..4556
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4557..4598
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 4910 AA; 559309 MW; E35EAF13BF6EC5ED CRC64;
MSQDRILLDL DVVNQRLILF NSAFPSDAIE APFHFSNKES TSENLDNLAG TILHSRSITG
HVFLYKHIFL EIVARWIKDS KKKDYVLVIE KLASIITIFP VAMPLIEDYL DKENDHFITI
LQNPSTQKDS DMFKILLAYY RLLYHNKEVF ARFIQPDILY QLVDLLTKEQ ENQVVIFLAL
KVLSLYLDMG EKTLNDMLDT YIKSRDSLLG HFEGDSGIDY SFLELNEAKR CANFSKLPSV
PECFTIEKKS SYFIIEPQDL STKVASICGV IVPKVHTIHD KVFYPLTFVP THKTVSSLRQ
LGRKIQNSTP IMLIGKAGSG KTFLINELSK YMGCHDSIVK IHLGEQTDAK LLIGTYTSGD
KPGTFEWRAG VLATAVKEGR WVLIEDIDKA PTDVLSILLS LLEKRELTIP SRGETVKAAN
GFQLISTVRI NEDHQKDSSN KIYNLNMIGM RIWNVIELEE PSEEDLTHIL AQKFPILTNL
IPKLIDSYKN VKSIYMNTKF ISLNKGAHTR VVSVRDLIKL CERLDILFKN NGINKPDQLI
QSSVYDSIFS EAADCFAGAI GEFKALEPII QAIGESLDIA SSRISLFLTQ HVPTLENLDD
SIKIGRAVLL KEKLNIQKKS MNSTLFAFTN HSLRLMEQIS VCIQMTEPVL LVGETGTGKT
TVVQQLAKML AKKLTVINVS QQTETGDLLG GYKPVNSKTV AVPIQENFET LFNATFSLKK
NEKFHKMLHR CFNKNQWKNV VKLWNEAYKM AQSILKITNT ENENENAKKK KRRLNTHEKK
LLLDKWADFN DSVKKFEAQS SSIENSFVFN FVEGSLVKTI RAGEWLLLDE VNLATADTLE
SISDLLTEPD SRSILLSEKG DAEPIKAHPD FRIFACMNPA TDVGKRDLPM GIRSRFTEIY
VHSPERDITD LLSIIDKYIG KYSVSDEWVG NDIAELYLEA KKLSDNNTIV DGSNQKPHFS
IRTLTRTLLY VTDIIHIYGL RRSLYDGFCM SFLTLLDQKS EAILKPVIEK FTLGRLKNVK
SIMSQTPPSP GPDYVQFKHY WMKKGPNTIQ EQAHYIITPF VEKNMMNLVR ATSGKRFPVL
IQGPTSSGKT SMIKYLADIT GHKFVRINNH EHTDLQEYLG TYVTDDTGKL SFKEGVLVEA
LRKGYWIVLD ELNLAPTDVL EALNRLLDDN RELFIPETQE VVHPHPDFLL FATQNPPGIY
GGRKILSRAF RNRFLELHFD DIPQDELEII LRERCQIAPS YAKKIVEVYR QLSIERSASR
LFEQKNSFAT LRDLFRWALR DAVGYEQLAA SGYMLLAERC RTPQEKVTVK KTLEKVMKVK
LDMDQYYASL EDKSLEAIGS VTWTKGMRRL SVLVSSCLKN KEPVLLVGET GCGKTTICQL
LAQFMGRELI TLNAHQNTET GDILGAQRPV RNRSEIQYKL IKSLKTALNI ANDQDVDLKE
LLQLYSKSDN KNIAEDVQLE IQKLRDSLNV LFEWSDGPLI QAMRTGNFFL LDEISLADDS
VLERLNSVLE PERSLLLAEQ GSSDSLVTAS ENFQFFATMN PGGDYGKKEL SPALRNRFTE
IWVPSMEDFN DVNMIVSSRL LEDLKDLANP IVKFSEWFGK KLGGGNATSG VISLRDILAW
VEFINKVFPK IQNKSTALIQ GASMVFIDAL GTNNTAYLAE NENDLKSLRT ECIIQLLKLC
GDDLELQQIE TNEIIVTQDE LQVGMFKIPR FPDAQSSSFN LTAPTTASNL VRVVRAMQVH
KPILLEGSPG VGKTSLITAL ANITGNKLTR INLSEQTDLV DLFGADAPGE RSGEFLWHDA
PFLRAMKKGE WVLLDEMNLA SQSVLEGLNA CLDHRGEAYI PELDISFSCH PNFLVFAAQN
PQYQGGGRKG LPKSFVNRFS VVFIDMLTSD DLLLIAKHLY PSIEPDIIAK MIKLMSTLED
QVCKRKLWGN SGSPWEFNLR DTLRWLKLLN QYSICEDVDV FDFVDIIVKQ RFRTISDKNK
AQLLIEDIFG KFSTKENFFK LTEDYVQINN EVALRNPHYR YPITQNLFPL ECNVAVYESV
LKAINNNWPL VLVGPSNSGK TETIRFLASI LGPRVDVFSM NSDIDSMDIL GGYEQVDLTR
QISYITEELT NIVREIISMN MKLSPNATAI MEGLNLLKYL LNNIVTPEKF QDFRNRFNRF
FSHLEGHPLL KTMSMNIEKM TEIITKEASV KFEWFDGMLV KAVEKGHWLI LDNANLCSPS
VLDRLNSLLE IDGSLLINEC SQEDGQPRVL KPHPNFRLFL TMDPKYGELS RAMRNRGVEI
YIDELHSRST AFDRLTLGFE LGENIDFVSI DDGIKKIKLN EPDMSIPLKH YVPSYLSRPC
IFAQVHDILL LSDEEPIEES LAAVIPISHL GEVGKWANNV LNCTEYSEKK IAERLYVFIT
FLTDMGVLEK INNLYKPANL KFQKALGLHD KQLTEETVSL TLNEYVLPTV SKYSDKIKSP
ESLYLLSSLR LLLNSLNALK LINEKSTHGK IDELTYIELS AAAFNGRHLK NIPRIPIFCI
LYNILTVMSE NLKTESLFCG SNQYQYYWDL LVIVIAALET AVTKDEARLR VYKELIDSWI
ASVKSKSDIE ITPFLNINLE FTDVLQLSRG HSITLLWDIF RKNYPTTSNS WLAFEKLINL
SEKFDKVRLL QFSESYNSIK DLMDVFRLLN DDVLNNKLSE FNLLLSKLED GINELELISN
KFLNKRKHYF ADEFDNLIRY TFSVDTAELI KELAPASSLA TQKLTKLITN KYNYPPIFDV
LWTEKNAKLT SFTSTIFSSQ FLEDVVRKSN NLKSFSGNQI KQSISDAELL LSSTIKCSPN
LLESQMEYYK NMLLSWLRKV IDIHVGGDCL KLTLKELCSL IEEKTASETR VTFAEYIFPA
LDLAESSKSL EELGEAWITF GTGLLLLFVP DSPYDPAIHD YVLYDLFLKT KTFSQNLMKS
WRNVRKVISG DEEIFTEKLI NTISDDDAPQ SPRVYRTGMS IDSLFDEWMA FLSSTMSSRQ
IKELVSSYKC NSDQSDRRLE MLQQNSAHFL NRLESGYSKF ADLNDILAGY IYSINFGFDL
LKLQKSKDRA SFQISPLWSM DPINISCAEN VLSAYHELSR FFKKGDMEDT SIEKVLMYFL
TLFKFHKRDT NLLEIFEAAL YTLYSRWSVR RFRQEQEENE KSNMFKFNDN SDDYEADFRK
LFPDYEDTAL VTNEKDISSP ENLDDIYFKL ADTYISVFDK DHDANFSSEL KSGAIITTIL
SEDLKNTRIE ELKSGSLSAV INTLDAETQS FKNTEVFGNI DFYHDFSIPE FQKAGDIIET
VLKSVLKLLK QWPEHATLKE LYRVSQEFLN YPIKTPLARQ LQKIEQIYTY LAEWEKYASS
EVSLNNTVKL ITDLIVSWRK LELRTWKGLF NSEDAKTRKS IGKWWFYLYE SIVISNFVSE
KKETAPNATL LVSSLNLFFS KSTLGEFNAR LDLVKAFYKH IQLIGLRSSK IAGLLHNTIK
FYYQFKPLID ERITNGKKSL EKEIDDIILL ASWKDVNVDA LKQSSRKSHN NLYKIVRKYR
DLLNGDAKTI IEAGLLYSNE NKLKLPTLKQ HFYEDPNLEA SKNLVKEIST WSMRAAPLRN
IDTVASNMDS YLEKISSQEF PNFADLASDF YAEAERLRKE TPNVYTKENK KRLAYLKTQK
SKLLGDALKE LRRIGLKVNF REDIQKVQSS TTTILANIAP FNNEYLNSSD AFFFKILDLL
PKLRSAASNP SDDIPVAAIE RGMALAQSLM FSLITVRHPL SEFTNDYCKI NGMMLDLEHF
TCLKGDIVHS SLKANVDNVR LFEKWLPSLL DYAAQTLSVI SKYSATSEQQ KILLDAKSTL
SSFFVHFNSS RIFDSSFIES YSRFELFINE LLKKLENAKE TGNAFVFDII IEWIKANKGG
PIKKEQKRGP SVEDVEQAFR RTFTSIILSF QKVIGDGIES ISETDDNWLS ASFKKVMVNV
KLLRSSVVSK NIETALSLLK DFDFTTTESI YVKSVISFTL PVITRYYNAM TVVLERSRIY
YTNTSRGMYI LSTILHSLAK NGFCSPQPPS EEVDDKNLQE GTGLGDGEGA QNNNKDVEQD
EDLTEDAQNE NKEQQDKDER DDENEDDAVE MEGDMAGELE DLSNGEENDD EDTDSEEEEL
DEEIDDLNED DPNAIDDKMW DDKASDNSKE KDTDQNLDGK NQEEDVQAAE NDEQQRDNKE
GGDEDPNAPE DGDEEIENDE NAEEENDVGE QEDEVKDEEG EDLEANVPEI ETLDLPEDMN
LDSEHEESDE DVDMSDGMPD DLNKEEVGNE DEEVKQESGI ESDNENDEPG PEEDAGETET
ALDEEEGAEE DVDMTNDEGK EDEENGPEEQ AMSDEEELKQ DAAMEENKEK GGEQNTEGLD
GVEEKADTED IDQEAAVQQD SGSKGAGADA TDTQEQDDVG GSGTTQNTYE EDQEDVTKNN
EESREEATAA LKQLGDSMKE YHRRRQDIKE AQTNGEEDEN LEKNNERPDE FEHVEGANTE
TDTQALGSAT QDQLQTIDED MAIDDDREEQ EVDQKELVED ADDEKMDIDE EEMLSDIDAH
DANNDVDSKK SGFIGKRKSE EDFENELSNE HFSADQEDDS EIQSLIENIE DNPPDASASL
TPERSLEESR ELWHKSEIST ADLVSRLGEQ LRLILEPTLA TKLKGDYKTG KRLNMKRIIP
YIASQFRKDK IWLRRTKPSK RQYQIMIALD DSKSMSESKC VKLAFDSLCL VSKTLTQLEA
GGLSIVKFGE NIKEVHSFDQ QFSNESGARA FQWFGFQETK TDVKKLVAES TKIFERARAM
VHNDQWQLEI VISDGICEDH ETIQKLVRRA RENKIMLVFV IIDGITSNES ILDMSQVNYI
PDQYGNPQLK ITKYLDTFPF EFYVVVHDIS ELPEMLSLIL RQYFTDLASS
//
