ID N1ZMU7_9LACO Unreviewed; 1164 AA.
AC N1ZMU7;
DT 26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2013, sequence version 1.
DT 24-JAN-2024, entry version 48.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=C821_01484 {ECO:0000313|EMBL:EMZ18322.1};
OS Lactobacillus sp. ASF360.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lactobacillus.
OX NCBI_TaxID=97137 {ECO:0000313|EMBL:EMZ18322.1, ECO:0000313|Proteomes:UP000012594};
RN [1] {ECO:0000313|EMBL:EMZ18322.1, ECO:0000313|Proteomes:UP000012594}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ASF360 {ECO:0000313|EMBL:EMZ18322.1,
RC ECO:0000313|Proteomes:UP000012594};
RX PubMed=24723722;
RA Wannemuehler M.J., Overstreet A.M., Ward D.V., Phillips G.J.;
RT "Draft genome sequences of the altered schaedler flora, a defined bacterial
RT community from gnotobiotic mice.";
RL Genome Announc. 2:e00287-e00214(2014).
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EMZ18322.1}.
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DR EMBL; AQFR01000131; EMZ18322.1; -; Genomic_DNA.
DR AlphaFoldDB; N1ZMU7; -.
DR STRING; 97137.C821_01484; -.
DR PATRIC; fig|97137.3.peg.1337; -.
DR eggNOG; COG1197; Bacteria.
DR HOGENOM; CLU_005122_1_3_9; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000012594; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}.
FT DOMAIN 629..790
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 807..965
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1164 AA; 133241 MW; 78436ADD8759C506 CRC64;
MRLTNYLEKD KDLNNFIDKT KDVKNSMITG ANAGAFSLLL KQIVNERKAP LVLIEENEHK
AQDLYGKLSA ILPEGQVQIF PVDDMIATQT ATTSPDELSS RIQTLNFLLS NKPGIVVTTP
AGLQYKLSKP EEYLAFKKTF DLDSEYDLKE LNDWLVRAGY HKESLVGKSG EFAIRGDILD
IYPLDRENPV RIEFFGDEID SIKEFDLSTQ RSQKELDKLT IFAAHDRVFS RDNLKKAAEK
IRKAMANAPA PEKAVKDHFT QSLDELDAGG LPNNYAFLVD YLISRPSSLL EYLSKDGLVF
LDDWPLINQS VKNVDSENAA FIDDELKTGA MLPGQELRLN FKRVWSKDSH HRIYFSLFQR
SLGRIKLDQM LDWQTREPEQ FFSQMPLIKT EIESYQKNAQ TVILQADNEK RAKQMSQNFA
DFGLDIPIVN KDDLIEKRTQ IVVGEFSEGF NLPNINLIYL TERELFNKRV HHKKRIKTLE
NAQRLRNYTE LKPGDYVVHV NHGIGRFEGI KTLENNGVKR DYITITYQRG DQLFVPADQL
KLVQKYVGSE GKRPHLNKLG GSEWAKTKRK VQSKVEDIAD DLIELYAKRE SEKGFAFSPD
DDLQRQFESA FPYVETADQL RSTKEIKEDM ESSKPMDRLV VGDVGFGKTE VALRAAFKAV
QDNKQVAFLV PTTILAQQHY ETILDRFKDF PVNVAMLSRF QTPAETKEII EKLKKGEIDI
VVGTHRILSK DVEFKNLGLL IIDEEQRFGV KHKERLKQLK ANIDVLTLTA TPIPRTLHMS
MVGVRDLSVM ETPPQNRYPI QTYVMEELPS VVKNACQREM QRGGQVFYLH NRIGDIDEVV
NKLEKLMPNA RIASIHGRMS QNQIEDILYR FLNREFDILV TTTIIETGID MPNVNTMIIE
DADKYGLSQL YQLRGRIGRS ARLAYAYFLY QPNKVLTEVG EKRLDAIRDF TELGSGFKIA
MRDLSIRGAG NMLGSQQHGF IDSVGYDLYS QMLSDAIKAR KNKKVIKKSN SEIDLGFESY
IPDAYIPDQE EKIEFYKKIK AVTSEEELDQ IEDELIDRFG DYPTSVENLL ASSKLKLNAD
NAQALNVVKK DDKIKVEFDI KASKELEGPN IFKALEHVSF KARISMTKDK KMVVLLLLPD
KLNNRMLFNE LTTFLEDASE IVQK
//
