ID N4WV23_9BACI Unreviewed; 386 AA.
AC N4WV23;
DT 26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2013, sequence version 1.
DT 24-JAN-2024, entry version 24.
DE RecName: Full=Bacterial Ig-like domain-containing protein {ECO:0000259|Pfam:PF07532};
GN ORFNames=J416_01884 {ECO:0000313|EMBL:ENH98205.1};
OS Gracilibacillus halophilus YIM-C55.5.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Gracilibacillus.
OX NCBI_TaxID=1308866 {ECO:0000313|EMBL:ENH98205.1, ECO:0000313|Proteomes:UP000012283};
RN [1] {ECO:0000313|EMBL:ENH98205.1, ECO:0000313|Proteomes:UP000012283}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=YIM-C55.5 {ECO:0000313|EMBL:ENH98205.1,
RC ECO:0000313|Proteomes:UP000012283};
RA Sugumar T., Polireddy D.R., Antony A., Madhava Y.R., Sivakumar N.;
RT "Draft genome sequence of Gracibacillus halophilus YIM-C55.5, a moderately
RT halophilic and thermophilic organism from the Xiaochaidamu salt lake.";
RL Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ENH98205.1}.
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DR EMBL; APML01000005; ENH98205.1; -; Genomic_DNA.
DR RefSeq; WP_003463478.1; NZ_APML01000005.1.
DR AlphaFoldDB; N4WV23; -.
DR STRING; 1308866.J416_01884; -.
DR PATRIC; fig|1308866.3.peg.382; -.
DR eggNOG; COG3940; Bacteria.
DR OrthoDB; 177947at2; -.
DR Proteomes; UP000012283; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd18817; GH43f_LbAraf43-like; 1.
DR InterPro; IPR016828; Alpha-L-arabinofuranosidase.
DR InterPro; IPR011081; Big_4.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR43817; GLYCOSYL HYDROLASE; 1.
DR PANTHER; PTHR43817:SF1; HYDROLASE, FAMILY 43, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G01660)-RELATED; 1.
DR Pfam; PF07532; Big_4; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR PIRSF; PIRSF025414; Alpha-L-arabinofuranosidase; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|RuleBase:RU361187};
KW Hydrolase {ECO:0000256|RuleBase:RU361187};
KW Reference proteome {ECO:0000313|Proteomes:UP000012283}.
FT DOMAIN 8..61
FT /note="Bacterial Ig-like"
FT /evidence="ECO:0000259|Pfam:PF07532"
FT ACT_SITE 76
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 250
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 191
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 386 AA; 44464 MW; 9AF4BFB60FFC66FA CRC64;
MNVKRLHEVE VTTNVGEVPE LPTSVTAELE GDVRATFNVS WNAIDPSKLD QPGTFEITGN
VQHKHYPKPF IEQRADPYLY KHSDGYYYFT GSYPLYDRVV LRRAKSLDEL PQAEEKVIWW
KHESGIMSEH IWAPEIHYIN GKWYIHVAAG DKDDIWAIRP YVLECEADNP LEGEWVEKGQ
VNTDFQSFSL DATTFEHQGK RYLVWAQKVD NDTISNLYIG EMENPWTIKN QILLSTPEYD
WERIGFDVNE GPAMIKSDQR IFIAFSASAT DENYAVGLLH ADINSDLLDP NAWTKEKEPV
FVSSDETSEY GPGHNSFSVD EEGNDLLIYH ARPYKGFNTE NPLYDHNRHA RIQRLFWTND
GKPYFGKPGD TIDFSNHPAK AIIRVK
//
