ID N8VKW7_9GAMM Unreviewed; 321 AA.
AC N8VKW7; A0A7T7WJM2;
DT 26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2013, sequence version 1.
DT 24-JAN-2024, entry version 35.
DE RecName: Full=3-carboxyethylcatechol 2,3-dioxygenase {ECO:0000256|ARBA:ARBA00013140};
DE EC=1.13.11.16 {ECO:0000256|ARBA:ARBA00013140};
GN ORFNames=F969_01088 {ECO:0000313|EMBL:ENV00176.1}, IAQ69_01650
GN {ECO:0000313|EMBL:QQN88418.1};
OS Acinetobacter variabilis.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales; Moraxellaceae;
OC Acinetobacter.
OX NCBI_TaxID=70346 {ECO:0000313|EMBL:ENV00176.1, ECO:0000313|Proteomes:UP000013070};
RN [1] {ECO:0000313|EMBL:ENV00176.1, ECO:0000313|Proteomes:UP000013070}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NIPH 899 {ECO:0000313|EMBL:ENV00176.1,
RC ECO:0000313|Proteomes:UP000013070};
RG The Broad Institute Genome Sequencing Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Cerqueira G., Feldgarden M., Courvalin P., Perichon B.,
RA Grillot-Courvalin C., Clermont D., Rocha E., Yoon E.-J., Nemec A.,
RA Walker B., Young S.K., Zeng Q., Gargeya S., Fitzgerald M., Haas B.,
RA Abouelleil A., Alvarado L., Arachchi H.M., Berlin A.M., Chapman S.B.,
RA Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M., Howarth C.,
RA Imamovic A., Larimer J., McCowan C., Murphy C., Neiman D., Pearson M.,
RA Priest M., Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J.,
RA Nusbaum C., Birren B.;
RT "The Genome Sequence of Acinetobacter sp. NIPH 899.";
RL Submitted (FEB-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:QQN88418.1, ECO:0000313|Proteomes:UP000596079}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=XM9F202-2 {ECO:0000313|EMBL:QQN88418.1,
RC ECO:0000313|Proteomes:UP000596079};
RA Peng K., Li R.;
RT "Emergence of ISAba1-mediated novel tet(X) in Acinetobacter variabilis from
RT a chicken farm.";
RL Submitted (AUG-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + O2 = (2Z,4E,7E)-2-
CC hydroxy-6-oxonona-2,4,7-trienedioate + H(+); Xref=Rhea:RHEA:25054,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:58642,
CC ChEBI:CHEBI:66888; EC=1.13.11.16;
CC Evidence={ECO:0000256|ARBA:ARBA00001748};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-(2,3-dihydroxyphenyl)propanoate + O2 = (2Z,4E)-2-hydroxy-6-
CC oxonona-2,4-dienedioate + H(+); Xref=Rhea:RHEA:23840,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:46951,
CC ChEBI:CHEBI:66887; EC=1.13.11.16;
CC Evidence={ECO:0000256|ARBA:ARBA00001843};
CC -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropanoate degradation.
CC {ECO:0000256|ARBA:ARBA00005207}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|ARBA:ARBA00011881}.
CC -!- SIMILARITY: Belongs to the LigB/MhpB extradiol dioxygenase family.
CC {ECO:0000256|ARBA:ARBA00007030}.
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DR EMBL; APPE01000041; ENV00176.1; -; Genomic_DNA.
DR EMBL; CP060811; QQN88418.1; -; Genomic_DNA.
DR RefSeq; WP_004781819.1; NZ_KB849402.1.
DR AlphaFoldDB; N8VKW7; -.
DR PATRIC; fig|1217710.3.peg.1030; -.
DR eggNOG; COG3384; Bacteria.
DR HOGENOM; CLU_078149_0_0_6; -.
DR UniPathway; UPA00714; -.
DR Proteomes; UP000013070; Unassembled WGS sequence.
DR Proteomes; UP000596079; Chromosome.
DR GO; GO:0047070; F:3-carboxyethylcatechol 2,3-dioxygenase activity; IEA:UniProtKB-EC.
DR GO; GO:0008198; F:ferrous iron binding; IEA:InterPro.
DR GO; GO:0019380; P:3-phenylpropionate catabolic process; IEA:UniProtKB-UniPathway.
DR CDD; cd07365; MhpB_like; 1.
DR Gene3D; 3.40.830.10; LigB-like; 1.
DR InterPro; IPR023789; DHPP/DHXA_dioxygenase.
DR InterPro; IPR004183; Xdiol_dOase_suB.
DR Pfam; PF02900; LigB; 1.
DR SUPFAM; SSF53213; LigB-like; 1.
PE 3: Inferred from homology;
KW Aromatic hydrocarbons catabolism {ECO:0000256|ARBA:ARBA00022797};
KW Dioxygenase {ECO:0000256|ARBA:ARBA00022964, ECO:0000313|EMBL:QQN88418.1};
KW Oxidoreductase {ECO:0000313|EMBL:QQN88418.1}.
FT DOMAIN 5..313
FT /note="Extradiol ring-cleavage dioxygenase class III enzyme
FT subunit B"
FT /evidence="ECO:0000259|Pfam:PF02900"
SQ SEQUENCE 321 AA; 35105 MW; A62B53C086608781 CRC64;
MAVKLICASH SPLMEFASPQ EKRKEQAVRE AFEKLSAEVK AYDPTLIITF GPDHFNGFFY
DLMPSFCVGI RATAAGDWDY GKDNAHIDVP EDKALGLVRR VLDEGVDVAY SYRMQADHGV
TQPLHFLCDG KLDRYPTIPI FINGAAAPMP TTKRTIALGR AVGQFIKSLN LENERVLVLG
TGGLSHDPPT PQMGSVPPEV EEFLIAGRNP TPEARNARQS KIIAVGQKLA AGDTSVAVPL
NAEWDIALLE KFKNADFAAL EAMTEAEIRR DGGRGGQEVR SWMAAFAALS EMGEYEMTTH
CYEEISEWIA GFGIVSAELK G
//
