ID N9VGL9_9GAMM Unreviewed; 1147 AA.
AC N9VGL9;
DT 26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2013, sequence version 1.
DT 24-JAN-2024, entry version 59.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=G114_16665 {ECO:0000313|EMBL:ENY70753.1};
OS Aeromonas diversa CDC 2478-85.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
OC Aeromonadaceae; Aeromonas.
OX NCBI_TaxID=1268237 {ECO:0000313|EMBL:ENY70753.1, ECO:0000313|Proteomes:UP000023775};
RN [1] {ECO:0000313|EMBL:ENY70753.1, ECO:0000313|Proteomes:UP000023775}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=2478-85 {ECO:0000313|EMBL:ENY70753.1,
RC ECO:0000313|Proteomes:UP000023775};
RX PubMed=23792745; DOI=10.1128/genomeA.00330-13;
RA Farfan M., Spataro N., Sanglas A., Albarral V., Loren J.G., Bosch E.,
RA Fuste M.C.;
RT "Draft Genome Sequence of the Aeromonas diversa Type Strain.";
RL Genome Announc. 1:S69-S82(2013).
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ENY70753.1}.
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DR EMBL; APVG01000056; ENY70753.1; -; Genomic_DNA.
DR RefSeq; WP_005359181.1; NZ_CDCE01000037.1.
DR AlphaFoldDB; N9VGL9; -.
DR PATRIC; fig|1268237.3.peg.3270; -.
DR eggNOG; COG1197; Bacteria.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000023775; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR CDD; cd18810; SF2_C_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11140; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR048635; MFD_D3.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF21132; MFD_D3; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}.
FT DOMAIN 614..775
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 797..950
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1147 AA; 128217 MW; 0FB1BBA8D17A675E CRC64;
MTRLSLPALP RKAGLKLTLG NLTGCALALT VSELARQHDH PILLVTADTP SALRLEQELA
FLLEGSDCPV LLFPDWETLP YDTFSPHQDI LSQRLETLYR LPTMAQGIII VPVTTLMLRC
PPRSYLDRFS LQLKQGQRLS LQGLRSRLAE AGYVAVEQVL EHGEFAARGS LLDLFPMGSQ
TPYRIDFFDD EVDSIRPFDP ETQRSRDPVA RVNLLPAREF PTDEAAIERF RGHYREHFEI
SRADGAIYQE VSKGRWPAGI EYYLPLFFDE TARLFDYLPA QSLLVTVGDT HGAAQRFWGD
IQQRYEDRRW DLSRPLLPPM ALYLPVEQLF ASLGDYAWVT LQEGAGTPHD LALTRLPELT
LDHSQEQPLA ALQHFLQGFA GRVLFTVPSE GRREALKDLL VRIDLRPGTV PTLEHFLAGD
DALAILVCPL EGGVILPDGK VAIITESELL GGRVRSQRKR ERAKQLSSDA VIRNLGELTQ
GQSVVHLDHG IGRYLGLETL SAGGLPTEFL TLEYAGGDKL FVPVTSLHLI SRYTGSDNPP
CHKLGGEAWT RAKRKAAEKV RDVAAELLDI YAHRAARSGF AFRHEREAYR QFCAGFPFEE
TEDQLNAINA VLGDMCQAKS MDRLVCGDVG FGKTEVAMRA AFVAVHGGKQ VAVLVPTTLL
AQQHYDNFRD RFANWPVRVE VLSRFRSAKE QSSVMKELAD GKVDIIIGTH KLLGSDLTFK
DLGLLIVDEE HRFGVRQKEK IKALRADVDI LTLTATPIPR TLNMAMSGMR DLSIIATPPA
KRLAIKTFVR QHDPAVVREA VLRELKRGGQ VYYLHNDVES IDKCASDLAE LLPEARISVA
HGQMRERELE RIMSDFYHQR FNLLVCTTII ETGIDVPSAN TIIMDRADHL GLAQLHQLRG
RVGRSHHQAY AYLLTPHPKL MTKDAAKRLE AIASLEDLGA GFALATHDLE IRGAGELLGD
DQSGQIESVG FTLYMEMLEQ AVEALKSGKE PALDTLLSDH TEVELRLPAL LPDDYLPDVN
MRLSMYKRIA SCASQEELDE LKVELIDRFG LLPEATKTLL EMAALRLGAT HLGIKRLEMS
ERGGYLDFSE KTSVDPAFLV RLLTEQPRIY KMEGPSRLRF NVVTGDRESR LRVVGDLLAE
LSRHKMA
//
