ID NAT1_ARATH Reviewed; 520 AA.
AC Q9SHZ3;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 27-MAR-2024, entry version 124.
DE RecName: Full=Nucleobase-ascorbate transporter 1;
DE Short=AtNAT1;
GN Name=NAT1; OrderedLocusNames=At2g05760; ORFNames=T25M19.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY, AND TISSUE SPECIFICITY.
RX PubMed=16982705; DOI=10.1093/pcp/pcl011;
RA Maurino V.G., Grube E., Zielinski J., Schild A., Fischer K., Flugge U.-I.;
RT "Identification and expression analysis of twelve members of the
RT nucleobase-ascorbate transporter (NAT) gene family in Arabidopsis
RT thaliana.";
RL Plant Cell Physiol. 47:1381-1393(2006).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in cotyledons 4 days after imbibition
CC (DAI). Expressed in the minor and major veins of cotyledons and leaves,
CC in the shoot apex and pedicels. Expressed in the root meristems, root
CC tips and lateral root primordia. {ECO:0000269|PubMed:16982705}.
CC -!- SIMILARITY: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC
CC 2.A.40) family. {ECO:0000305}.
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DR EMBL; AC007233; AAD26910.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC05969.1; -; Genomic_DNA.
DR EMBL; BT023436; AAY56427.1; -; mRNA.
DR PIR; D84471; D84471.
DR RefSeq; NP_178636.1; NM_126592.3.
DR AlphaFoldDB; Q9SHZ3; -.
DR SMR; Q9SHZ3; -.
DR STRING; 3702.Q9SHZ3; -.
DR PaxDb; 3702-AT2G05760-1; -.
DR ProteomicsDB; 251249; -.
DR EnsemblPlants; AT2G05760.1; AT2G05760.1; AT2G05760.
DR GeneID; 815127; -.
DR Gramene; AT2G05760.1; AT2G05760.1; AT2G05760.
DR KEGG; ath:AT2G05760; -.
DR Araport; AT2G05760; -.
DR TAIR; AT2G05760; NAT1.
DR eggNOG; KOG1292; Eukaryota.
DR HOGENOM; CLU_017959_5_3_1; -.
DR InParanoid; Q9SHZ3; -.
DR OMA; LPFFGCQ; -.
DR OrthoDB; 345287at2759; -.
DR PhylomeDB; Q9SHZ3; -.
DR PRO; PR:Q9SHZ3; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SHZ3; baseline and differential.
DR Genevisible; Q9SHZ3; AT.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0071702; P:organic substance transport; IEA:UniProt.
DR InterPro; IPR006043; NCS2.
DR NCBIfam; NF037981; NCS2_1; 1.
DR PANTHER; PTHR11119:SF3; LD30822P; 1.
DR PANTHER; PTHR11119; XANTHINE-URACIL / VITAMIN C PERMEASE FAMILY MEMBER; 1.
DR Pfam; PF00860; Xan_ur_permease; 1.
PE 2: Evidence at transcript level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..520
FT /note="Nucleobase-ascorbate transporter 1"
FT /id="PRO_0000270158"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 64..84
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 86..106
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 129..149
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 150..170
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 174..194
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 213..233
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 279..299
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 362..382
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 384..404
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 415..435
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 453..473
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 520 AA; 57131 MW; 20CF2827A8FE8C97 CRC64;
MAEISHPPME QLQDLEYCID SNPPWPETVL LAFQNYILML GTSAFIPALL VPAMGGSDGD
RARVIQTLLF VAGIKTLLQA LFGTRLPAVV GGSLAYVVPI AYIINDSSLQ KISNDHERFI
HTMRAIQGAL IVASSIQIIL GYSQVWGLFS RFFSPLGMAP VVGLVGLGMF QRGFPQLGNC
IEIGLPMLLL VIGLTQYLKH VRPFKDVPIF ERFPILICVT IVWIYAVILT ASGAYRGKPS
LTQHSCRTDK ANLISTAPWF KFPYPLQWGP PTFSVGHSFA MMSAVLVSMV ESTGAYIAAS
RLAIATPPPA YVLSRGIGWQ GIGVLLDGLF GTGTGSTVLV ENVGLLGLTR VGSRRVVQVS
AGFMIVFSTL GKFGAVFASI PVPIYAALHC ILFGLVAAVG LSFLQFTNMN SMRNLMITGL
SLFLGISIPQ FFAQYWDARH YGLVHTNAGW FNAFLNTLFM SPATVGLIIA VFMDNTMEVE
RSKKDRGMPW WVKFRTFRGD NRNEEFYTLP FNLNRFFPPT
//
