ID NIFA_HERSE Reviewed; 542 AA.
AC P27713;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1992, sequence version 1.
DT 27-MAR-2024, entry version 120.
DE RecName: Full=Nif-specific regulatory protein;
GN Name=nifA;
OS Herbaspirillum seropedicae.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Oxalobacteraceae; Herbaspirillum.
OX NCBI_TaxID=964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 35893 / DSM 6446 / LMG 6514 / Z78;
RX PubMed=1840608; DOI=10.1099/00221287-137-7-1511;
RA Souza E.M., Funayama S., Rigo L.U., Yates M.G., Pedrosa F.O.;
RT "Sequence and structural organization of a nif A-like gene and part of a
RT nifB-like gene of Herbaspirillum seropedicae strain Z78.";
RL J. Gen. Microbiol. 137:1511-1522(1991).
CC -!- FUNCTION: Required for activation of most nif operons, which are
CC directly involved in nitrogen fixation.
CC -!- SUBUNIT: Interacts with sigma-54.
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DR EMBL; M60319; AAA25027.1; -; Genomic_DNA.
DR PIR; A44813; A44813.
DR AlphaFoldDB; P27713; -.
DR SMR; P27713; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR GO; GO:0045893; P:positive regulation of DNA-templated transcription; IMP:CACAO.
DR CDD; cd00009; AAA; 1.
DR Gene3D; 1.10.8.60; -; 1.
DR Gene3D; 3.30.450.40; -; 1.
DR Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003018; GAF.
DR InterPro; IPR029016; GAF-like_dom_sf.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR002197; HTH_Fis.
DR InterPro; IPR010113; Nif-specific_regulatory_prot.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR002078; Sigma_54_int.
DR InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR InterPro; IPR025943; Sigma_54_int_dom_ATP-bd_2.
DR InterPro; IPR025944; Sigma_54_int_dom_CS.
DR NCBIfam; TIGR01817; nifA; 1.
DR PANTHER; PTHR32071; TRANSCRIPTIONAL REGULATORY PROTEIN; 1.
DR PANTHER; PTHR32071:SF95; TRANSCRIPTIONAL REGULATORY PROTEIN ZRAR; 1.
DR Pfam; PF01590; GAF; 1.
DR Pfam; PF02954; HTH_8; 1.
DR Pfam; PF00158; Sigma54_activat; 1.
DR PRINTS; PR01590; HTHFIS.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00065; GAF; 1.
DR SUPFAM; SSF55781; GAF domain-like; 1.
DR SUPFAM; SSF46689; Homeodomain-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE 4: Predicted;
KW Activator; ATP-binding; DNA-binding; Metal-binding; Nitrogen fixation;
KW Nucleotide-binding; Transcription; Transcription regulation;
KW Two-component regulatory system.
FT CHAIN 1..542
FT /note="Nif-specific regulatory protein"
FT /id="PRO_0000081307"
FT DOMAIN 29..170
FT /note="GAF"
FT DOMAIN 203..432
FT /note="Sigma-54 factor interaction"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT DNA_BIND 514..533
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT REGION 433..499
FT /note="Inter-domain linker"
FT REGION 500..542
FT /note="C-terminal DNA-binding domain"
FT BINDING 231..238
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT BINDING 294..303
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT BINDING 446
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000250|UniProtKB:P05407"
FT BINDING 451
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000250|UniProtKB:P05407"
SQ SEQUENCE 542 AA; 60880 MW; 3A0BD14B14390AA5 CRC64;
MATILDDRSV NLELVTIYEI SKILGSSLDL SKTLREVLNV LSAHLETKRV LLSLMQDSGE
LQLVSAIGLS YEEFQSGRYR VGEGITGKIF QTETPIVVRD LAQEPLFLAR TSPRQSQDGE
VISFVGVPIK AAREMLGVLC VFRDGQSPSR SVDHEVRLLT MVANLIGQTV RLYRSVAAER
QQLQEEKRQL SRQLQGKYKL DNVIGISKAM QEVFAQVHQS APSRSTMLLR GESGTGKEVI
ARAIHYLSPR KDGPFIKVNC AALSETLLES ELFGHEKGAF TGAQGERKGR FELAHGGTLF
LDEIGEISPA FQAKLLRVLQ EREFERVGGS RSIKVDVRLV TATNRDLEKA VAKGEFRADL
YYRINVVSIF IPPLRERRED IPYLVEHFLE KFRVENQRAM VAMSPQAMKV MMNCYWPGNV
RELENCVERT ATMMRGDLIT EVHFSCQQNK CLTKVLHEPG QQQPVVVVPL ERISAPYGAI
FAEWDGQGQA TGAAPPTSER ERLIWAMEQC GWVQAKAARA LNISPRQMGY ALQKFNIEVK
KF
//
