GenomeNet

Database: UniProt
Entry: NIFH_METAR
LinkDB: NIFH_METAR
Original site: NIFH_METAR 
ID   NIFH_METAR              Reviewed;         274 AA.
AC   Q0W443;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   28-FEB-2018, entry version 75.
DE   RecName: Full=Nitrogenase iron protein {ECO:0000255|HAMAP-Rule:MF_00533};
DE            EC=1.18.6.1 {ECO:0000255|HAMAP-Rule:MF_00533};
DE   AltName: Full=Nitrogenase Fe protein {ECO:0000255|HAMAP-Rule:MF_00533};
DE   AltName: Full=Nitrogenase component II {ECO:0000255|HAMAP-Rule:MF_00533};
DE   AltName: Full=Nitrogenase reductase {ECO:0000255|HAMAP-Rule:MF_00533};
GN   Name=nifH {ECO:0000255|HAMAP-Rule:MF_00533};
GN   OrderedLocusNames=UNCMA_13850; ORFNames=RCIX1606;
OS   Methanocella arvoryzae (strain DSM 22066 / NBRC 105507 / MRE50).
OC   Archaea; Euryarchaeota; Methanomicrobia; Methanocellales;
OC   Methanocellaceae; Methanocella.
OX   NCBI_TaxID=351160;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 22066 / NBRC 105507 / MRE50;
RX   PubMed=16857943; DOI=10.1126/science.1127062;
RA   Erkel C., Kube M., Reinhardt R., Liesack W.;
RT   "Genome of rice cluster I archaea -- the key methane producers in the
RT   rice rhizosphere.";
RL   Science 313:370-372(2006).
CC   -!- FUNCTION: The key enzymatic reactions in nitrogen fixation are
CC       catalyzed by the nitrogenase complex, which has 2 components: the
CC       iron protein and the molybdenum-iron protein. {ECO:0000255|HAMAP-
CC       Rule:MF_00533}.
CC   -!- CATALYTIC ACTIVITY: 8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP
CC       + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16
CC       phosphate. {ECO:0000255|HAMAP-Rule:MF_00533}.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00533};
CC       Note=Binds 1 [4Fe-4S] cluster per dimer. {ECO:0000255|HAMAP-
CC       Rule:MF_00533};
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00533}.
CC   -!- PTM: The reversible ADP-ribosylation of Arg-97 inactivates the
CC       nitrogenase reductase and regulates nitrogenase activity.
CC       {ECO:0000255|HAMAP-Rule:MF_00533}.
CC   -!- SIMILARITY: Belongs to the NifH/BchL/ChlL family.
CC       {ECO:0000255|HAMAP-Rule:MF_00533}.
DR   EMBL; AM114193; CAJ36850.1; -; Genomic_DNA.
DR   RefSeq; WP_012035713.1; NC_009464.1.
DR   ProteinModelPortal; Q0W443; -.
DR   SMR; Q0W443; -.
DR   STRING; 351160.RCIX1606; -.
DR   EnsemblBacteria; CAJ36850; CAJ36850; RCIX1606.
DR   GeneID; 5144036; -.
DR   KEGG; rci:RCIX1606; -.
DR   PATRIC; fig|351160.9.peg.1430; -.
DR   eggNOG; arCOG00590; Archaea.
DR   eggNOG; COG1348; LUCA.
DR   HOGENOM; HOG000228826; -.
DR   KO; K02588; -.
DR   OMA; CQKVIVV; -.
DR   OrthoDB; POG093Z00RZ; -.
DR   BioCyc; MARV351160:G1GJJ-1422-MONOMER; -.
DR   Proteomes; UP000000663; Chromosome.
DR   GO; GO:0016612; C:molybdenum-iron nitrogenase complex; IEA:InterPro.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0018697; F:carbonyl sulfide nitrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016163; F:nitrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   CDD; cd02040; NifH; 1.
DR   HAMAP; MF_00533; NifH; 1.
DR   InterPro; IPR030655; NifH/chlL_CS.
DR   InterPro; IPR000392; NifH/frxC.
DR   InterPro; IPR005977; Nitrogenase_Fe_NifH.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR42864; PTHR42864; 1.
DR   Pfam; PF00142; Fer4_NifH; 1.
DR   PIRSF; PIRSF000363; Nitrogenase_iron; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01287; nifH; 1.
DR   PROSITE; PS00746; NIFH_FRXC_1; 1.
DR   PROSITE; PS00692; NIFH_FRXC_2; 1.
DR   PROSITE; PS51026; NIFH_FRXC_3; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; ADP-ribosylation; ATP-binding; Complete proteome; Iron;
KW   Iron-sulfur; Metal-binding; Nitrogen fixation; Nucleotide-binding;
KW   Oxidoreductase; Reference proteome.
FT   CHAIN         1    274       Nitrogenase iron protein.
FT                                /FTId=PRO_1000211894.
FT   NP_BIND       8     15       ATP. {ECO:0000255|HAMAP-Rule:MF_00533}.
FT   METAL        94     94       Iron-sulfur (4Fe-4S); shared with dimeric
FT                                partner. {ECO:0000255|HAMAP-
FT                                Rule:MF_00533}.
FT   METAL       129    129       Iron-sulfur (4Fe-4S); shared with dimeric
FT                                partner. {ECO:0000255|HAMAP-
FT                                Rule:MF_00533}.
FT   MOD_RES      97     97       ADP-ribosylarginine; by dinitrogenase
FT                                reductase ADP-ribosyltransferase.
FT                                {ECO:0000255|HAMAP-Rule:MF_00533}.
SQ   SEQUENCE   274 AA;  29714 MW;  AFF8E737F3A2B0F9 CRC64;
     MRQVAIYGKG GIGKSTTTQN TVAALAESGK KVMVVGCDPK ADSTRLLLHG LNQKTVLDTL
     RDEGDDIELE SILKTGFGET RCVESGGPEP GVGCAGRGII TSINMLEQLG AYTDDLDYVF
     YDVLGDVVCG GFAMPIREGK AREIYIVASG ELMALYAANN IAKGIKKYAE TGGVRLGGII
     CNSRKADNEY ALVKAVAEEI GTQMIHFVPR DNIVQRAEIN KMTVIDFDPA ANQANEYRKL
     AKAIDENQMF VVPKPMTQDR LEELMMKHGF LDAV
//
DBGET integrated database retrieval system