ID O05212_AZOSP Unreviewed; 511 AA.
AC O05212;
DT 01-JUL-1997, integrated into UniProtKB/TrEMBL.
DT 01-JUL-1997, sequence version 1.
DT 24-JAN-2024, entry version 76.
DE RecName: Full=Nitrogenase molybdenum-iron protein beta chain {ECO:0000256|ARBA:ARBA00014775, ECO:0000256|RuleBase:RU364127};
DE EC=1.18.6.1 {ECO:0000256|ARBA:ARBA00012773, ECO:0000256|RuleBase:RU364127};
DE AltName: Full=Dinitrogenase {ECO:0000256|RuleBase:RU364127};
DE Flags: Fragment;
GN Name=nifK {ECO:0000313|EMBL:CAA60580.1};
OS Azoarcus sp.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales; Zoogloeaceae;
OC Azoarcus.
OX NCBI_TaxID=29544 {ECO:0000313|EMBL:CAA60580.1};
RN [1] {ECO:0000313|EMBL:CAA60580.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=BH72 {ECO:0000313|EMBL:CAA60580.1};
RX PubMed=8709842; DOI=10.1111/j.1365-2958.1995.mmi_18020225.x;
RA Hurek T., Van Montagu M., Kellenberger E., Reinhold-Hurek B.;
RT "Induction of complex intracytoplasmic membranes related to nitrogen
RT fixation in Azoarcus sp. BH72.";
RL Mol. Microbiol. 18:225-236(1995).
RN [2] {ECO:0000313|EMBL:CAA60580.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=BH72 {ECO:0000313|EMBL:CAA60580.1};
RA Reinhold-Hurek B.;
RL Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: This molybdenum-iron protein is part of the nitrogenase
CC complex that catalyzes the key enzymatic reactions in nitrogen
CC fixation. {ECO:0000256|ARBA:ARBA00002621,
CC ECO:0000256|RuleBase:RU364127}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16
CC ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16
CC phosphate; Xref=Rhea:RHEA:21448, Rhea:RHEA-COMP:10000, Rhea:RHEA-
CC COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17997,
CC ChEBI:CHEBI:18276, ChEBI:CHEBI:28938, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:456216; EC=1.18.6.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000805,
CC ECO:0000256|RuleBase:RU364127};
CC -!- COFACTOR:
CC Name=[8Fe-7S] cluster; Xref=ChEBI:CHEBI:21143;
CC Evidence={ECO:0000256|RuleBase:RU364127};
CC Note=Binds 1 [8Fe-7S] cluster per heterodimer.
CC {ECO:0000256|RuleBase:RU364127};
CC -!- SUBUNIT: Tetramer of two alpha and two beta chains. Forms complex with
CC the iron protein (nitrogenase component 2).
CC {ECO:0000256|ARBA:ARBA00011462, ECO:0000256|RuleBase:RU364127}.
CC -!- SIMILARITY: Belongs to the NifD/NifK/NifE/NifN family.
CC {ECO:0000256|ARBA:ARBA00011002, ECO:0000256|RuleBase:RU004021}.
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DR EMBL; X87103; CAA60580.1; -; Genomic_DNA.
DR AlphaFoldDB; O05212; -.
DR GO; GO:0016612; C:molybdenum-iron nitrogenase complex; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0018697; F:carbonyl sulfide nitrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016163; F:nitrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR CDD; cd01974; Nitrogenase_MoFe_beta; 1.
DR Gene3D; 3.40.50.1980; Nitrogenase molybdenum iron protein domain; 3.
DR Gene3D; 1.20.89.10; Nitrogenase Molybdenum-iron Protein, subunit B, domain 4; 1.
DR InterPro; IPR000510; Nase/OxRdtase_comp1.
DR InterPro; IPR000318; Nase_comp1_CS.
DR InterPro; IPR005976; Nase_Mo-Fe_CF_bsu.
DR InterPro; IPR024564; Nase_Mo-Fe_CF_bsu_N.
DR NCBIfam; TIGR01286; nifK; 1.
DR PANTHER; PTHR33712; LIGHT-INDEPENDENT PROTOCHLOROPHYLLIDE REDUCTASE SUBUNIT B; 1.
DR PANTHER; PTHR33712:SF7; LIGHT-INDEPENDENT PROTOCHLOROPHYLLIDE REDUCTASE SUBUNIT B; 1.
DR Pfam; PF11844; DUF3364; 1.
DR Pfam; PF00148; Oxidored_nitro; 1.
DR SUPFAM; SSF53807; Helical backbone' metal receptor; 1.
DR PROSITE; PS00699; NITROGENASE_1_1; 1.
DR PROSITE; PS00090; NITROGENASE_1_2; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|RuleBase:RU364127};
KW Iron {ECO:0000256|RuleBase:RU364127};
KW Iron-sulfur {ECO:0000256|RuleBase:RU364127};
KW Metal-binding {ECO:0000256|RuleBase:RU364127};
KW Nitrogen fixation {ECO:0000256|ARBA:ARBA00023231,
KW ECO:0000256|RuleBase:RU004021};
KW Nucleotide-binding {ECO:0000256|RuleBase:RU364127};
KW Oxidoreductase {ECO:0000256|RuleBase:RU364127}.
FT DOMAIN 2..55
FT /note="Nitrogenase molybdenum-iron protein beta chain N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF11844"
FT DOMAIN 69..500
FT /note="Nitrogenase/oxidoreductase component 1"
FT /evidence="ECO:0000259|Pfam:PF00148"
FT NON_TER 511
FT /evidence="ECO:0000313|EMBL:CAA60580.1"
SQ SEQUENCE 511 AA; 57888 MW; 2641F8811671D58F CRC64;
MQAVDDIKPC YPLFRDDDYK ENLKRKQDLY EERVPADKIA ETFEWTTSKE YQELNFKREA
LTINPAKACQ PLGAVLCSLG FKNTMPYVHG SQGCVAYFRT YFNRHFKEPV SCVSDSMTED
AAVFGGQKNM FDGLENAKAI YKPDMIAVST TCMAEVIGDD LNAFINNAKK AGHVPQEYPV
PFAHTPSFVG SHTTGWDNMW EGIARFFTLN KMEGKVVGSN GKLNFVPGFE TYLGNYRVIK
RMMGQMGVDA TVLSDPSEVL DTPSDGEFRM YSGGTTMDEM SDAPNALNTI VLQPWQLEKS
KKFVETVWNH DVPKLNIPMG LDWTDEFLMK VSEITGKAIP EELALERGRL VDMMQDSHTW
LHGKKFALYG DPDFVLGLTK ILLELGAEPI HILSNNANKR WGKAVQKVLD GYKHGANCKV
YVGNDLWHMR SLCFTEKPDF LIGNSYGKYI QRDTVFKGKE FEVPLIRLGF PIFDRHHLHR
MTTLGYEGAM YVLTTLVNAV LERLDDETRG M
//
